Electrochemical solid-state nanosensor based on a dual amplification strategy for sensitive detection of (FeIII-dopamine)

Esfahani, Sepehr Lajevardi; Rouhani, Shohre; Ranjbar, Zahra

doi:10.1016/j.electacta.2019.01.065

Cited by 11 publications

(6 citation statements)

References 60 publications

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“…In terms of response, the proposed aptasensor approach clearly outperforms previously reported methods. Indeed, because of their advantages, such as simple instrumentation, rapidness, excellent compatibility with miniaturized technologies, and their remarkable sensitivity to the target, electrochemical aptasensor-based methods have more applications compared to other sensing technologies [ 56 , 57 ].…”

Section: Resultsmentioning

confidence: 99%

“…RIV-specific aptamers were created via the SELEX method to screen a library of more than 10 14 single-stranded DNA. In addition, a label-free electrochemical aptasensor was created as a biosensing platform for detecting RIV using this aptamer, which is capable of sensing RIV at the 1 nM level [ 56 ]. The aptamer was immobilized by a self-assembly procedure on the surface of ITO-PET modified by gold nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

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Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis

et al. 2022

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For the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5′-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT CAC TAG TGA ATT-3′ displayed a high binding affinity to RIV and had an efficient ability to discriminate RIV from similar molecular structures. A novel label-free electrochemical aptasensor was designed and fabricated through the conjugation of a thiolated aptamer with Au nanoparticles (Au-NPs). Then, the aptasensor was successfully applied for the quantitative determination of RIV in human plasma and exhaled breath condensate (EBC) samples with limits of detection (LODs) of 14.08 and 6.03 nM, respectively. These valuable results provide ample evidence of the green electrogeneration of AuNPs on the surface of electrodes and their interaction with loaded aptamers (based on Au-S binding) towards the sensitive and selective monitoring of RIV in human plasma and EBC samples. This bio-assay is an alternative approach for the clinical analysis of RIV and has improved specificity and affinity. As far as we know, this is the first time that an electrochemical aptasensor has been verified for the recognition of RIV and that allows for the easy, fast, and precise screening of RIV in biological samples.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis

et al. 2022

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“…The scheme of generating a signal from the aptasensor is also becoming more and more complicated. In the literature, there are examples of using additional light irradiation [ 28 , 29 , 30 ], signal amplification due to additional components (nanoparticles, graphene, etc.) [ 31 , 32 , 33 ], ratiometric schemes [ 34 ], and so on.…”

Section: Introductionmentioning

confidence: 99%

“…[ 31 , 32 , 33 ], ratiometric schemes [ 34 ], and so on. However, the more complex the system, the less reproducible and stable it is when in use [ 30 ]. Furthermore, an improved understanding of the key factors in the origin and response formation of the biosensor signal is an important step towards developing advanced electrochemical sensing platforms [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood

Shabalina

Sharko

Glazyrin

et al. 2021

Sensors

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We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer’s defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker’s presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics.

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“…During the layer-by-layer (LbL) assembly, the synthesized electroactive naphthalimide dye/LDH nanoplatelets matrix (NALD-n) displayed in successive layers on the GO/ITO/PET surface electrode. This structure design resulted in a significant increase in the fluorescence emission of electroactive dye in the sensor's bed [19].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer Assembly of Electroactive Dye/LDHs Nanoplatelet Matrix Film for Advanced Dual Electro-optical Sensing Applications

2020

Self Cite

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It proved that the most destructive effects of the toxic Al3+ ion on the human nervous system and disease that are involved with this system, such as Alzheimer's. The development of solid-state electrodes is still in its infancy during the sensor-based detection methods for Al3+. Hence, in this study, a novel flexible ITO/PET-based electrochemical solid-state sensor was designed and constructed. Modification of the surface of electrode bedding was done by layer-by-layer (LbL) assembly of Mg–Al LDH. nanoplatelets along with alizarin red S (ARS) in an interconnected matrix film. In the molecular design of sensing base of the electrode, the electroactive organic units (ARS molecules) present in the ITO/PET-layered (ARS/LDHs)n matrix are involved in electrochemical reactions when exposed to the target molecule (Al3+ ion), so the electrochemical changes of the new formed Al-chelated system are detectable. This type of sensor is used for sensitive and selective detection of Al3+. The minimum sheet resistance, morphology and high electrocatalytic activity of the modified matrix film are obtained in the fifth cycle of LbL assembly technique. In this electrochemical sensor, both electrochemical and optical methods were detected with high sensitivity and selectivity of Al3+, so that in a cyclic voltammetry electrochemical method, the lower detection limit of 10.1 nM with a linear range of [0.2–120 μM] was obtained compared to the fluorescence-based optical method.

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Electrochemical solid-state nanosensor based on a dual amplification strategy for sensitive detection of (FeIII-dopamine)

Cited by 11 publications

References 60 publications

Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis

Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis

Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood

Layer-by-Layer Assembly of Electroactive Dye/LDHs Nanoplatelet Matrix Film for Advanced Dual Electro-optical Sensing Applications

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