“…According to the report by Lili Wei , DNA in acetate buffer solution will ganerate signal peaks of guanine and adenine by DPV. Based on this property, we investigated the electrochemical behavior of the interaction of MTX and DNA.…”
Section: Resultsmentioning
confidence: 99%
“…At the same time, the effect of pH value of supporting electrolyte on the voltammetric behavior of DNA/GO/GCE was also investigated. The choice of pH was principally based on the previous literature that oxidation peak current response of guanine was linear in the pH range of 4.0–5.6 . Figure (A) shows that the oxidation peak potential of guanine was shifted negatively with the increase of pH value.…”
Section: Resultsmentioning
confidence: 99%
“…Although the exposed glassy carbon electrode could detect MTX, but its detection limit was poor. Altough DNA Langmuir‐Blodgett films modified GCE and DNA/SWCNT/Nafion/GCE showed a low detection limit for MTX detection, but a Langmuir‐Blodgett instrument was required and CNTs enhanced the background current remarkably . In this paper, we have proposed a novel method for detecting MTX compared with the previous studies.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, carbon materials can also be combined with protein, DNA and other biological materials to construct biosensors . Recently, many GO‐based DNA electrochemical sensors have been developed for detection of the drug, protein, biological small molecules and other substances .…”
Methotrexate (MTX) was used as an anti-cancer drug, but its excessive use can cause serious side effects, it was necessary to monitor MTX in vivo. In this report, DNA was immobilized on a glassy carbon electrode (GCE) modified with graphene oxide (GO) to develop an electrochemical sensor for sensitive determination of MTX for the first time. The adsorptive voltammetric behaviors of MTX on DNA sensor were investigated using differential pulse voltammetry (DPV). The peak current response of guanine in DNA was used as a determination signal of MTX in acetate buffer solution pH 4.6. Voltammetric investigations revealed that the proposed method could determine MTX in the concentration range from 5.5 3 10 À8 to 2.2 3 10 À6 mol L À1 with a lower detection limit of 7.6 3 10 À 9 mol L À1 (S/N = 3). The method was applied to detect MTX in human blood serum and diluted urine samples with excellent recoveries of 97.4-102.5 %. Compared with the previous studies, the DNA/GO/GCE electrode constructed by us based on the change rate of guanine current (R%) in DNA, proportionally reflecting the MTX concentration, is simple and sensitive.
“…According to the report by Lili Wei , DNA in acetate buffer solution will ganerate signal peaks of guanine and adenine by DPV. Based on this property, we investigated the electrochemical behavior of the interaction of MTX and DNA.…”
Section: Resultsmentioning
confidence: 99%
“…At the same time, the effect of pH value of supporting electrolyte on the voltammetric behavior of DNA/GO/GCE was also investigated. The choice of pH was principally based on the previous literature that oxidation peak current response of guanine was linear in the pH range of 4.0–5.6 . Figure (A) shows that the oxidation peak potential of guanine was shifted negatively with the increase of pH value.…”
Section: Resultsmentioning
confidence: 99%
“…Although the exposed glassy carbon electrode could detect MTX, but its detection limit was poor. Altough DNA Langmuir‐Blodgett films modified GCE and DNA/SWCNT/Nafion/GCE showed a low detection limit for MTX detection, but a Langmuir‐Blodgett instrument was required and CNTs enhanced the background current remarkably . In this paper, we have proposed a novel method for detecting MTX compared with the previous studies.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, carbon materials can also be combined with protein, DNA and other biological materials to construct biosensors . Recently, many GO‐based DNA electrochemical sensors have been developed for detection of the drug, protein, biological small molecules and other substances .…”
Methotrexate (MTX) was used as an anti-cancer drug, but its excessive use can cause serious side effects, it was necessary to monitor MTX in vivo. In this report, DNA was immobilized on a glassy carbon electrode (GCE) modified with graphene oxide (GO) to develop an electrochemical sensor for sensitive determination of MTX for the first time. The adsorptive voltammetric behaviors of MTX on DNA sensor were investigated using differential pulse voltammetry (DPV). The peak current response of guanine in DNA was used as a determination signal of MTX in acetate buffer solution pH 4.6. Voltammetric investigations revealed that the proposed method could determine MTX in the concentration range from 5.5 3 10 À8 to 2.2 3 10 À6 mol L À1 with a lower detection limit of 7.6 3 10 À 9 mol L À1 (S/N = 3). The method was applied to detect MTX in human blood serum and diluted urine samples with excellent recoveries of 97.4-102.5 %. Compared with the previous studies, the DNA/GO/GCE electrode constructed by us based on the change rate of guanine current (R%) in DNA, proportionally reflecting the MTX concentration, is simple and sensitive.
“…With this easy concept, it was even possible to detect traceamounts (nM) in real water samples, making this biosensor a real alternative to common laborious methods such as high-performance liquid chromatography mass spectrometry (HPLC-MS) or gas chromatography mass spectrometry (GC-MS). The same concept could also be applied for the detection of phenol 257,258 and catechol 259,260 in the range of µM. Environmental toxins are not always the result of industrial production, but can also be produced by organisms such as pathogenic microbes.…”
Section: Other Biologically Relevant Analytesmentioning
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