Electrochemical sensor based on rGO/Au nanoparticles for monitoring H2O2 released by human macrophages

Patella, Bernardo; Buscetta, Marco; Vincenzo, Serena Di; Ferraro, Maria; Aiello, Giuseppe; Sunseri, C.; Pace, Elisabetta; Inguanta, Rosalinda; Cipollina, Chiara

doi:10.1016/j.snb.2020.128901

Cited by 95 publications

(45 citation statements)

References 69 publications

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“…According to our results and other studies [51] , [55] , macrophages possess a higher tolerance to ROS than other cells. In this antiviral system, Ag-TiO 2 SAN efficiently eliminated SARS-CoV2 pseudovirus in lysosomes of macrophages.…”

Section: Resultssupporting

confidence: 90%

TiO2 supported single Ag atoms nanozyme for elimination of SARS-CoV2

et al. 2021

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The outbreak of SARS-coronavirus 2 (SARS-CoV2) has become a global health emergency. Although enormous efforts have been made, there is still no effective treatment against the new virus. Herein, a TiO 2 supported single-atom nanozyme containing atomically dispersed Ag atoms (Ag-TiO 2 SAN) is designed to serve as a highly efficient antiviral nanomaterial. Compared with troditional nano-TiO 2 and Ag, Ag-TiO 2 SAN exhibits higher adsorption (99.65%) of SARS-CoV2 pseudovirus. This adsorption ability is due to the interaction between SAN and receptor binding domain (RBD) of spike 1 protein of SARS-CoV2. Theoretical calculation and experimental evidience indicate that the Ag atoms of SAN strongly bind to cysteine and asparagine, which are the most abundant amino acids on the surface of spike 1 RBD. After binding to the virus, the SAN/virus complex is typically phagocytosed by macrophages and colocalized with lysosomes. Interestingly, Ag-TiO 2 SAN possesses high peroxidase-like activity responsible for reactive oxygen species production under acid conditions. The highly acidic microenvironment of lysosomes could favor oxygen reduction reaction process to eliminate the virus. With hACE2 transgenic mice, Ag-TiO 2 SAN showed efficient anti-SARS-CoV2 pseudovirus activity. In conclusion, Ag-TiO 2 SAN is a promising nanomaterial to achieve effective antiviral effects for SARS-CoV2.

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Section: Resultssupporting

confidence: 90%

TiO2 supported single Ag atoms nanozyme for elimination of SARS-CoV2

et al. 2021

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“…Besides, monitoring the critical biomarkers in the human body represented an essential basis for health assessment, pharmaceutical guidance, surgical intervention protocols, and postsurgical monitoring (Khan et al, 2019 ; Li et al, 2020 ; Patella et al, 2021 ). Nowadays, there is a high interest in developing efficient strategies to diagnose cancer and fight against it.…”

Section: Nanopatterned Surface To Detect Cellular State and Chemicals Based On Electrochemical Techniquesmentioning

confidence: 99%

“…Increasing the generation of reactive oxygen species (ROS) leads to most inflammatory diseases' pathogenesis besides its association with aging and chronic inflammation. Hydrogen peroxide (H 2 O 2 ) received considerable attention due to the vital role of H 2 O 2 in directing the leukocytes to the injury site (Patella et al, 2021 ). Thus, the accurate monitoring of H 2 O 2 derived from living cells is of critical significance to control the progression of congenital diseases such as cancer and damage to DNA.…”

Section: Nanopatterned Surface To Detect Cellular State and Chemicals Based On Electrochemical Techniquesmentioning

confidence: 99%

“…Also, pencil graphite electrode (PGE) was modified with a poly(2-aminophenylbenzimidazole)/Au NPs (P2AB/Au NPs) and used as an H 2 O 2 sensor that could detected H 2 O 2 with no interference by ascorbic acid, dopamine, uric acid, or glucose (Teker et al, 2019 ). Additionally, Patella et al ( 2021 ) have used rGO/Au NPs/ITO/PET electrode as an H 2 O 2 sensor for direct detecting of H 2 O 2 that released by cells in culture supernatants (Patella et al, 2021 ).…”

Section: Nanopatterned Surface To Detect Cellular State and Chemicals Based On Electrochemical Techniquesmentioning

confidence: 99%

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Electrochemical Cell Chips Based on Functionalized Nanometals

El‐Said

Yoon

Lee³

2021

Front. Chem.

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The electrochemical technique is one of the most accurate, rapid, and sensitive analytical assays, which becomes promising techniques for biological assays at a single-cell scale. Nanometals have been widely used for modification of the traditional electrodes to develop highly sensitive electrochemical cell chips. The electrochemical cell chips based on the nanostructured surface have been used as label-free, simple, and non-destructive techniques for in vitro monitoring of the effects of different anticancer drugs at the cellular level. Here, we will provide the recent progress in fabrication of nanopatterned surface and cell-based nanoarray, and discuss their applications based on electrochemical techniques such as detection of cellular states and chemicals, and non-destructive monitoring of stem cell differentiation.

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“…Electrochemical methods are able to discern between different species by choice of the appropriate electrode potential. Amperometric sensors are particularly attractive alternative for popular methods of ROS detection based on fluorescent probes, biochemical colorimetric or fluorimetric assays [2–4] . Amperometry is fast, sensitive, and highly cost‐effective method.…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing the Electrocatalytic Properties of Graphene Oxide – Gold Nanoparticles Hybrid System

2021

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Graphene oxide (GO) – gold nanoparticles (AuNPs) nanocomposites are promising materials for H2O2 electrochemical sensors. Surface composition of GO is an important factor influencing the sensitivity. In this study GO has been mildly reduced by ammonia (GO‐NH3) or sodium hydroxide solution (GO‐NaOH). Such mild reduction opens up the epoxide groups on the GO surface but not the other oxygen functionalities. The electrochemical reduction of GO has been carried out too. It is found that the structure of the electrochemically reduced graphene oxide (ERGO) depends on the concentration of GO used to prepare the layer. For concentrated GO (10 mg/ml) the electro‐reduction leaves OH surface groups while for lower concentrations all surface groups can be removed. All GO materials listed above were combined with AuNPs and examined as possible amperometric H2O2 sensors. The two types of layers were examined: AuNPs deposited on the top of the GO material or AuNPs deposited under the GO material layer. Among studied composites ERGO obtained from 5 mg/ml GO on the AuNPs layer showed the highest sensitivity (155.6 μA cm−2 mM−1 at −0.4 V vs Ag/AgCl).

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Electrochemical sensor based on rGO/Au nanoparticles for monitoring H2O2 released by human macrophages

Cited by 95 publications

References 69 publications

TiO2 supported single Ag atoms nanozyme for elimination of SARS-CoV2

TiO2 supported single Ag atoms nanozyme for elimination of SARS-CoV2

Electrochemical Cell Chips Based on Functionalized Nanometals

Factors Influencing the Electrocatalytic Properties of Graphene Oxide – Gold Nanoparticles Hybrid System

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