Electrochemical Sensing of Biotin Using Nafion-Modified Boron-Doped Diamond Electrode

Buzid, Alyah; McGlacken, Gerard P.; Glennon, Jeremy D.; Luong, John H. T.

doi:10.1021/acsomega.8b01209

Cited by 28 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of studies have been reported on the potential utilization of avidin–biotin interaction in sensing technology. − Using various detection methods such as competitive immunoassay, electrochemical, cyclic voltammetry, and immunoaffinity chromatography, detection limits ranging from 2 pM to 84 nM have been reported (Table ). It seems that the present AuNP-GFET biosensor based on the avidin–biotin technology shows the highest sensitivity and quick detection time.…”

Section: Resultsmentioning

confidence: 99%

Avidin–Biotin Technology in Gold Nanoparticle-Decorated Graphene Field Effect Transistors for Detection of Biotinylated Macromolecules with Ultrahigh Sensitivity and Specificity

et al. 2020

View full text Add to dashboard Cite

The strong and specific noncovalent interaction between avidin and biotin is widely exploited in different types of enzyme-linked immunosorbent assay kits, labeled immunosensors, and polymer-based sensing devices for the detection of different biomarkers specific to different diseases such as cancer and influenza. Here, we employed the avidin–biotin technology in a novel gold nanoparticle-decorated graphene field-effect transistor (AuNP-GFET) and demonstrated the specific detection of the biotinylated macromolecules such as biotinylated proteins and nucleotides in the sub-picomolar (pM) range. The AuNP-GFET was constructed by fabricating six pairs of interdigital electrodes on graphene transferred on a SiO2/Si substrate. The sensing performance of AuNP-GFET was characterized by the real-time two-terminal electrical current measurement upon injection of the analyte solution into a silicone pool preattached onto the electrodes. Avidin, a tetrameric biotin-binding protein with strong affinity and specificity, immobilized on AuNP-decorated single-layer graphene, was used as the sensing platform and transduced the electrical signal upon binding to the analyte macromolecules. The sensing capability of the AuNP-GFET was tested with the biotinylated protein A. Sensitivity of the present biosensor was estimated to be ∼0.4 pM. The specificity and applicability of the biosensor were confirmed using both synthetic and real samples. Because the biotin label can retain its binding capability to avidin with strong affinity and specificity even after conjugating with varieties of proteins and nucleotides, the present AuNP-GFET biosensor is expected to promote the research in developing different biosensors.

show abstract

Section: Resultsmentioning

confidence: 99%

Avidin–Biotin Technology in Gold Nanoparticle-Decorated Graphene Field Effect Transistors for Detection of Biotinylated Macromolecules with Ultrahigh Sensitivity and Specificity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Thus, HPLC equipped with a downstream BDD electrode may be used for the separation and sensitive detection of BAK Full Paper homologs, formulated in pharmaceutical preparations [16]. If necessary, the electrode can be coated with Nafion [28], a negatively charged polymer to enhance the selective adsorption of BAK homologs. In this context, the electrode can be deposited with the negatively charged sulfobutylether-beta-cyclodextrin-doped poly(Nacetyltyramine) [29].…”

Section: Discussionmentioning

confidence: 99%

Electroanalysis of Benzalkonium Chloride in Ophthalmic Formulation by Boron‐doped Diamond Electrode

et al. 2021

Self Cite

View full text Add to dashboard Cite

Benzalkonium chloride (BAK) is a mixture of alkyl benzyl dimethyl ammonium chlorides, which is used primarily as a biocide, surfactant, preservative, and antimicrobial agent in the pharmaceutical industry, in particular in ophthalmologic and nasal solutions. However, BAK may cause harmful consequences on the eye structures of the anterior segment. Control of BAK identity and content is necessary by applying a sensitive detection method. This study unravels the use of a glassy carbon (GC) electrode and a pristine boron‐doped diamond electrode (BDD) for the detection of four BAK homologs in a non‐aqueous medium using square wave voltammetry (SWV). The BDD provided more reproducibility of the oxidation potential than GC with a correlation coefficient of 0.999. The irreversible oxidation peak was very broad and deconvoluted into 3 peaks corresponding to C12, C14, and combined C16–C18 to reflect their concentration ratio in the mixture. The method was then extended to the detection of the C12 homolog in the ophthalmic formulations with a limit of detection (LOD) of 0.4 μg/mL. The estimated BAK levels in three ophthalmic formulations were in agreement with the specified values by the manufacturers. The results were validated by high‐performance liquid chromatography (HPLC) with ultraviolet (UV) detection, confirming the presence of a single homolog (C12) in the eye drops.

show abstract

“…To date, a number of studies have reported on the utilization of avidin-biotin interaction in sensing technology [46][47][48][49][50]. The detection limits ranged from 2 pM to 84 nM have been reported using various detection methods such as competitive immunoassay, electrochemical, cyclic voltammetry, and immunoaffinity chromatography as listed in Table 5.1.…”

Section: Comparative Sensitivity and Practical Applicabilitymentioning

confidence: 99%

Graphene FET Biosensor Based on the Avidin–Biotin Technology

Wang

Hossain

Zhao

et al. 2021

Graphene Field-Effect Transistor Biosensors

View full text Add to dashboard Cite

Avidin-biotin interaction is the strongest known protein-ligand interaction and is widely used in the field of biomedicine, including immunochromatography and various biological detection technologies. Because avidin or streptavidin can specifically bind to four biotinylated molecules efficiently and quickly, and the biotinylated molecules will not change their inherent characteristics. At present, this avidin-biotin interaction has been widely used in the field of biomedical detection to amplify biological signals for specific molecules. Combined with the graphene fieldeffect transistor biosensor, using the avidin-biotin interaction, the concentration of specific molecules in the test solution is expected to be amplified by the biotin-avidin interaction, which helps to increase the limit of detection for the biosensor. Besides, based on the avidin-biotin interaction, the graphene field-effect transistor biosensor is expected to rapidly quantitative detect free biotin and biotinylated molecules. This chapter mainly introduces the graphene field-effect transistor biosensor from the perspectives of device preparation, surface modification, sensitivity, and specificity, and discusses the possible situations that the sensor may encounter in actual detection.Avidin-biotin technology is widely used in different types of ELISA (enzyme-linked immunosorbent assay) kits, polymer-based detection and labeled immunosensor s for the detection of different bio-markers linked to different diseases such as cancer and influenza. In this section, we introduced the employing avidin-biotin technology in the graphene FET biosensor and demonstrated the specific detection of the biotinylated biomolecule in the sub-pico molar (pM) range. The sensing performance of graphene FET biosensor was characterized by the real-time two-terminal electrical current measurement upon injection of analyte solution into a silicone pool preattached onto the graphene channel. Since the Avidin-biotin technology has strong affinity and specificity, any biotinylated biomolecules are hopping for rapid detecting with ultra-low concentration level through this sensing platform. Thus the present graphene FET biosensor is expected to be a breakthrough in biomedical analysis. It can be used as a potential common platform for the rapid and point of care detection of different biomolecules and biomarkers linked to different diseases.

show abstract

Electrochemical Sensing of Biotin Using Nafion-Modified Boron-Doped Diamond Electrode

Cited by 28 publications

References 43 publications

Avidin–Biotin Technology in Gold Nanoparticle-Decorated Graphene Field Effect Transistors for Detection of Biotinylated Macromolecules with Ultrahigh Sensitivity and Specificity

Avidin–Biotin Technology in Gold Nanoparticle-Decorated Graphene Field Effect Transistors for Detection of Biotinylated Macromolecules with Ultrahigh Sensitivity and Specificity

Electroanalysis of Benzalkonium Chloride in Ophthalmic Formulation by Boron‐doped Diamond Electrode

Graphene FET Biosensor Based on the Avidin–Biotin Technology

Contact Info

Product

Resources

About