Electrochemical recognition of taurine biomarker in unprocessed human plasma samples using silver nanoparticlebased nanocomposite: A new platform for early stage diagnosis of neurodegenerative diseases of the nervous system

Abstract

The rapid and accurate determination of the level of taurine biomarker in various tissues and body fluids can be of great interest in the early diagnosis of several important pathologies and diseases. In the present study, an innovative electrochemical interface for quantitation of taurine based on ternary signal amplification strategy was fabricated. In this work, silver nanoparticles were electrodeposited onto green and biocompatible nanocomposite containing α-cyclodextrin as conductive matrix. Therefore, a double layer film based on α-cyclodextrin and silver nanoparticles was exploited to develop a highly sensitive electrochemical sensor for detection of taurine. Fully electrochemical methodology was used to prepare a transducer on a glassy carbon electrode which provided a high surface area towards sensitive detection of taurine biomarker. The surface morphology of electrode surface was characterized by high-resolution field emission scanning electron microscope (FE-SEM). The proposed sensing platform provides a simple tool for taurine detection. The calibration curve for taurine concentration was linear in 0.7 nM to 0.1 mM with low limit of quantification of 0.7 nM. The practical analytical utility of the modified electrode was illustrated by determination of taurine in unprocessed human plasma samples with recovery of 90.8% to 104%.

Identification of taurine biomarker in human biofluids using plasmonic patterns of silver nanostructure

“…Most of the reported techniques offer several advantages, including low sensitivity, complex mobile phases, and time-consuming extraction procedures with limited applicability. A summary of the results obtained in this study compared with previously reported works is presented in Table 1, 39–45 showing that the developed method has certain advantages over previous approaches in terms of stability, suitable surface area, and feasible biological activity. Notably, our study's strengths include the utilization of a calorimetric chemosensing procedure to detect taurine in urine and blood samples for the first time.…”

Advances in Nanotechnology towards Development of Silver Nanoparticle-Based Wound-Healing Agents

“…These properties are notably important in their biomedical applications, such as antifungal [ 66 ], anticancer [ 67 ], antiviral [ 68 ], antiangiogenic [ 69 ], antileishmanial [ 70 ] anti-inflammatory [ 71 ] and antibacterial [ 72 ] activities. Due to the global increase in life threatening diseases and the emergence of multidrug resistance in pathogens against antibiotics [ 73 ], multiple medical applications have been demonstrated for AgNPs, such as sensors for disease diagnosis [ 74 ], nanocarriers for drug delivery [ 75 , 76 ], sensitisers in radiation therapy [ 77 ], electro spin resonance dosimetry, etc. [ 78 ].…”

“…Although AgNPs are well known for their antibacterial activity [ 62 ], research over the years has shown that these nanomaterials have other health-related beneficial properties. They are used in medical applications as diagnostic [ 74 ], drug delivery [ 75 , 76 ], sensitising and therapeutic agents. The global rise of the health, social and economic burden of wounds is a growing concern that necessitates novel strategies to produce sustainable solutions.…”