Electrochemical primer extension based on polyoxometalate electroactive labels for multiplexed detection of single nucleotide polymorphisms

Chahin, Nassif; Uribe, Laura A.; Debela, Ahmed M.; Thorimbert, Serge; Hasenknopf, Bernold; Ortiz, Mayreli; Katakis, Ioanis; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2018.06.014

Cited by 18 publications

(12 citation statements)

References 25 publications

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“…230 Most recently, the authors expanded the latter approach to explore the multiplexed detection of single nucleotide polymorphisms on a microfluidic electrode array. 231 Hybrid POMs have also been explored in combination with a range of live cell types (e.g. viruses, bacteria and/or mammalian) are of obvious specific interest in the design and targeting of these species as novel metallodrugs in particular (vide infra).…”

Section: Hybrid Pom Complexes With Biological Structuresmentioning

confidence: 99%

Supramolecular assemblies of organo-functionalised hybrid polyoxometalates: from functional building blocks to hierarchical nanomaterials

et al. 2022

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Section: Hybrid Pom Complexes With Biological Structuresmentioning

confidence: 99%

Supramolecular assemblies of organo-functionalised hybrid polyoxometalates: from functional building blocks to hierarchical nanomaterials

et al. 2022

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“…Results from the EndoChip were in good correlation to ELISA results using endogenous samples with less sample preparation and more rapid analysis [ 60 ]. The investigation of SNP variants in depression phenotypes is a burgeoning field, thus electrochemical systems have been designed to identify and distinguish a range of single-nucleotide polymorphisms [ 61 ]. Through the use of single-stranded DNA probes designed for each target SNP, multiplexed SNP detection can be expanded to incorporate other desired SNPs of interest, potentially aiding in the distinction between BDNF variants.…”

Section: Suggested Electrochemical Point-of-care Approachesmentioning

confidence: 99%

The emergence of psychoanalytical electrochemistry: the translation of MDD biomarker discovery to diagnosis with electrochemical sensing

et al. 2022

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The disease burden and healthcare costs of psychiatric diseases along with the pursuit to understand their underlying biochemical mechanisms have led to psychiatric biomarker investigations. Current advances in evaluating candidate biomarkers for psychiatric diseases, such as major depressive disorder (MDD), focus on determining a specific biomarker signature or profile. The origins of candidate biomarkers are heterogenous, ranging from genomics, proteomics, and metabolomics, while incorporating associations with clinical characterization. Prior to clinical use, candidate biomarkers must be validated by large multi-site clinical studies, which can be used to determine the ideal MDD biomarker signature. Therefore, identifying valid biomarkers has been challenging, suggesting the need for alternative approaches. Following validation studies, new technology must be employed to transition from biomarker discovery to diagnostic biomolecular profiling. Current technologies used in discovery and validation, such as mass spectroscopy, are currently limited to clinical research due to the cost or complexity of equipment, sample preparation, or measurement analysis. Thus, other technologies such as electrochemical detection must be considered for point-of-care (POC) testing with the needed characteristics for physicians’ offices. This review evaluates the advantages of using electrochemical sensing as a primary diagnostic platform due to its rapidity, accuracy, low cost, biomolecular detection diversity, multiplexed capacity, and instrument flexibility. We evaluate the capabilities of electrochemical methods in evaluating current candidate MDD biomarkers, individually and through multiplexed sensing, for promising applications in detecting MDD biosignatures in the POC setting.

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“…The coupling of hybrid WD Sn-substituted polyoxometalates with amine substrates through an amidation reaction was previously demonstrated. The carboxylic , or self-alkylated cyclic derivatives − allowed coupling of the primary and secondary amines to the WD POM, leading to hybrids with a 1:1 POM:amine ratio. However, the synthetic pathway for obtaining the bis-substituted WD POMs that are suitable for the reaction with amine ligands under mild conditions has been not described so far.…”

Section: Introductionmentioning

confidence: 99%

A Bis-organosilyl-Functionalized Wells–Dawson Polyoxometalate as a Platform for Facile Amine Postfunctionalization

2020

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The development of modular platforms that can undergo postfunctionalization reactions permits coupling of inorganic clusters with different organic functionalities, thereby expanding the range of key physicochemical properties that are relevant for applications in different areas of science. In this work, a novel hybrid Wells–Dawson polyoxometalate (POM) platform was developed and successfully used for postfunctionalization via a nucleophilic substitution reaction. Two new halogen-functionalized bis-organosilyl Wells–Dawson POMs TBA6[α2-P2W17O61{O(SiC3H6-X)2}] (X = Cl or I) were synthesized, and their coupling with amine substrates was explored in a one-step postfunctionalization reaction. The iodide form of the POM has proven to be much more reactive, and its reaction with a range of primary and secondary amines resulted in a series of new bis-substituted Wells–Dawson POMs with the general formula TBA6[α2-P2W17O61{O(SiC3H6-NR1R2)2}]. Coupling of 18 amines with R1 and R2 groups, which exhibited a wide variety in terms of both chemical nature and bulkiness, was achieved under mild conditions via a catalyst-free approach. Using Na2CO3 as a base in acetonitrile solutions at 55 °C resulted in hybrid products that were obtained in high purity and good yields, after a simple isolation and purification procedure.

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Electrochemical primer extension based on polyoxometalate electroactive labels for multiplexed detection of single nucleotide polymorphisms

Cited by 18 publications

References 25 publications

Supramolecular assemblies of organo-functionalised hybrid polyoxometalates: from functional building blocks to hierarchical nanomaterials

Supramolecular assemblies of organo-functionalised hybrid polyoxometalates: from functional building blocks to hierarchical nanomaterials

The emergence of psychoanalytical electrochemistry: the translation of MDD biomarker discovery to diagnosis with electrochemical sensing

A Bis-organosilyl-Functionalized Wells–Dawson Polyoxometalate as a Platform for Facile Amine Postfunctionalization

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