Electrochemical Paper-Based Nanobiosensor for Rapid and Sensitive Detection of Monkeypox Virus

Exploring monkeypox virus proteins and rapid detection techniques

“…To ensure optimal use in POC settings, electrochemical devices must be reproducible and easily accessible, allowing them to be disposable and removing the requirement for trained personnel ( Torres et al., 2021 ). de Lima et al. (2023) reported the first electrochemical POC assay for MPXV protein detection using a paper-based laser-scribed graphene (LSG) nanobiosensor ( Figure 4A ).…”

“…The analytical curves for A29 protein and MPXV viral loads at various concentrations showed a linear correlation with a determination coefficient ( R 2 ) of 0.998 and 0.996, respectively ( Figures 4B, C ). Additionally, no instances of cross-reaction were detected when the nanobiosensor was tested alongside other poxvirus and non-poxvirus strains ( de Lima et al., 2023 ).…”

“… (A) Schematic illustration of an LSG nanobiosensor-based electrochemical assay for MPXV detection linked to a portable potentiostat controlled via a smartphone; (B) The analytical curve presenting normalized resistance to charge transfer ( R CT ) values against the logarithm of the A29 protein concentration; (C) The analytical curve presenting the normalized R CT values against the logarithm of the titered MPXV sample (adapted with permission from de Lima et al., 2023 ). …”

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“…The main advantage of this nanobiosensor is its simplicity of use; only 2.5 μl of the sample is required, and anyone can perform the test without any sophisticated lab equipment or trained technicians, using just the potentiostat and a smartphone. 55…”

Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus

“…Additionally, besides the several types of electrochemical biosensors for virus detection that have been reported in the literature, to our knowledge, there is only one study reporting the detection of MKPV using an electrochemical sensor. Lima et al, 2023, 29 developed a nanostructured paper-based biosensor modified with a CO 2 laser for detecting the MKPV A29 protein. The electrochemical biosensor was able to successfully demonstrate the determination of the protein in human saliva and serum samples, thus demonstrating the potential applicability of an electrochemical sensor for MKPV detection.…”