Electrochemical oxidation of the antitumor antibiotic mitomycin C and in situ evaluation of its interaction with DNA using a DNA-electrochemical biosensor

Bruzaca, Evellin Enny S.; Lopes, Ilanna C.; Silva, Elizaura Hyeda C.; Carvalho, Paulina Andréa Viana de; Tanaka, Auro Atsushi

doi:10.1016/j.microc.2017.03.030

Cited by 21 publications

(8 citation statements)

References 26 publications

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“…A dsDNA-electrochemical biosensor consists of an electrochemical transducer with dsDNA immobilized on its surface and have been largely employed for evaluation of DNA-hazard compounds reaction mechanisms and/or detection and quantification of DNA bases oxidative damage products [28][29][30][31][32][33][34][35][36][37]. Usually, different adsorption immobilization procedures, electrostatic adsorption or evaporation, of a monolayer or multilayer DNA films have been used [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Guanine‐methylation Using Glassy Carbon Electrode

et al. 2019

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This work presents a study of the electrochemical oxidation of 7‐methylguanine (7‐mGua) in aqueous solution at glassy carbon electrode by cyclic voltammetry, differential pulse voltammetry, square wave voltammetry and electrochemical impedance spectrometry. The anodic behaviour of 7‐mGua was compared with the electro‐oxidation of guanine and 7‐methylguanosine. The results demonstrated that the methyl and ribose groups are not electroactive but strongly influence the oxidation mechanism of these species. The oxidation of 7‐mGua occurred in a single pH‐dependent step, with the withdrawal of two electrons and two protons of C8, to form 8‐oxo‐7‐methylguanine, while the electro‐oxidation of 7‐methylguanosine also occurred in a single pH‐dependent step, however, with the withdrawal of one electron and one proton of C8 to form a hydroxylated product, since its oxidation to 8‐oxo‐7‐methylguanosine is hindered by the presence of the pendant groups. In addition, the oxidation of 7‐mGua was investigated in the presence of DNA and DNA‐bases, leading to the conclusion that the formation of 7‐mGua, from an interaction of DNA with an alkylating agent, would cause an increase on the deoxyguanosine peak current of the DNA‐biosensor, with no interference of any free DNA bases, which demonstrated that DNA‐electrochemical biosensors find application on detecting DNA methylation, opening a new avenue for applications of DNA biosensors.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Guanine‐methylation Using Glassy Carbon Electrode

et al. 2019

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“…The stationary working electrode is composed of various types of electrode materials. The most common are glassy carbon electrodes (GCEs) [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ], pencil graphite electrodes (PGEs) [ 50 , 51 , 52 , 53 , 54 , 55 , 56 ], carbon paste electrodes (CPEs) [ 57 , 58 ], hanging mercury drop electrodes (HMDEs) [ 59 ], platinum electrodes [ 60 ], and gold electrodes [ 61 , 62 ]. These electrodes can be used either in the bare form [ 44 , 54 , 63 ] or modified with a layer capable of increasing their sensitivity [ 50 , 51 , 52 , 53 , 55 , 56 ].…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

“…Consequently, the impact of the subsequent portions (increasing the DNA concentration) on the redox processes of the examined system is determined [ 24 , 26 , 44 , 46 , 59 , 63 ]. An alternative method is the modification of the electrode, which involves immobilizing one of the analyzed system elements (studied compound with biological activity [ 64 ] or DNA [ 41 , 42 , 57 , 61 , 62 , 63 , 65 , 66 ]) on the surface of the working electrode. The last-mentioned approach is often used to obtain DNA biosensor systems for monitoring drug interactions.…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

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The interactions of compounds with DNA have been studied since the recognition of the role of nucleic acid in organisms. The design of molecules which specifically interact with DNA sequences allows for the control of the gene expression. Determining the type and strength of such interaction is an indispensable element of pharmaceutical studies. Cognition of the therapeutic action mechanisms is particularly important for designing new drugs. Owing to their sensitivity, simplicity, and low costs, electrochemical methods are increasingly used for this type of research. Compared to other techniques, they require a small number of samples and are characterized by a high reliability. These methods can provide information about the type of interaction and the binding strength, as well as the damage caused by biologically active molecules targeting the cellular DNA. This review paper summarizes the various electrochemical approaches used for the study of the interactions between pharmaceuticals and DNA. The main focus is on the papers from the last decade, with particular attention on the voltammetric techniques. The most preferred experimental approaches, the electrode materials and the new methods of modification are presented. The data on the detection ranges, the binding modes and the binding constant values of pharmaceuticals are summarized. Both the importance of the presented research and the importance of future prospects are discussed.

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“…It is well-known that DNA is one of the major target for anticancer drugs due to their ability to interfere with cell growth and division [1][2][3][4][5]. For these reasons, investigation of the interaction between DNA and anticancer drugs is one of the key topics for drug discovery and drug dose determination studies [6][7][8][9]. With this aim in mind, in this paper, investigation of double-stranded DNA (dsDNA) with Mitomycin C (MMC) using electroactive polymer coated electrodes was demonstrated and detailed.…”

Section: Introductionmentioning

confidence: 99%

“…Interaction has been followed by the changes in the oxidation signal of guanine base of dsDNA. Bruzaca et al have studied electrochemical oxidation of Mitomycin C and its interaction with DNA on an electrochemical biosensor platform using cyclic voltammetry, square wave voltammetry (SWV) and differential pulse voltammetry at carbon paste electrode [9].…”

Section: Introductionmentioning

confidence: 99%

Modified Graphite Surfaces Prepared for Electrochemical Biomolecular Interaction Detection Studies

Kuralay

2019

Hacettepe Journal of Biology and Chemistry

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B u çalışma, çift sarmal DNA (dsDNA) ile önemli ve sık kullanılan bir antikanser ilacı olan Mitomisin C (MMC) arasındaki biyomoleküler etkileşimin görüntülenmesi için elektroaktif polimer modifiye elektrot malzemelerinin hazırlanmasını göstermektedir. Modifiye elektrot malzemeleri, o-fenilendiamin (oPD) monomerinin nanomalzeme içeren bir çözeltide elektropolimerizasyonu ile oluşturulmuştur. Katkı maddesi (dopant) molekül olarak kullanılan nanomalzeme grafen (GN)'dir ve elektropolimerizasyon tekniği dönüşümlü voltametri (CV)'dir. Sonrasında poli(o-fenilendiamin) polimer modifiye yüzeylere dsDNA immobilizasyonu gerçekleştirilmiştir. Oluşturulan nanomalzeme katılmış bu polimer modifiye elektrotlar, dsDNA-MMC etkileşiminin tespitinde biyotayin platformları olarak kullanılmışlardır. Biyomoleküler etkileşimlerini aydınlatmak için farklı MMC etkileşim süreleri çalışılmıştır.

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Electrochemical oxidation of the antitumor antibiotic mitomycin C and in situ evaluation of its interaction with DNA using a DNA-electrochemical biosensor

Cited by 21 publications

References 26 publications

Electrochemical Detection of Guanine‐methylation Using Glassy Carbon Electrode

Electrochemical Detection of Guanine‐methylation Using Glassy Carbon Electrode

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

Modified Graphite Surfaces Prepared for Electrochemical Biomolecular Interaction Detection Studies

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