Electrochemical oxidation of NADH at a bare glassy carbon electrode in different supporting electrolytes

Katekawa, Edson; Maximiano, Flávio Antonio; Rodrigues, Luciane L; Delbem, Maria Flávia; Serrano, Sı́lvia Helena Pires

doi:10.1016/s0003-2670(98)00694-1

Cited by 18 publications

(18 citation statements)

References 42 publications

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“…GCE/CB had Ep,a similar to O-GCE (0.442 V), while GCE/MWCNT (0.085) and GCE/O-MWCNT (0.054 V) had very low voltammetric anodic peaks. The decrease in oxidation overpotential and also the k 0 values reported in Table 1 implies a catalytic effect of the materials, being more significant for MWCNT and O-MWCNT, in agreement with the literature [2,8,25,26]. The data obtained for GCE/CB are also in agreement with the literature, Arduini et al [9] demonstrated, using a screen-printed electrode modified with CB, the CB ability to reduce the Ep,a for the NADH oxidation.…”

Section: Electrochemical Response For Nadh Using Differentsupporting

confidence: 88%

NADH Oxidation onto Different Carbon-Based Sensors: Effect of Structure and Surface-Oxygenated Groups

et al. 2018

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Different carbon-based materials have been compared for the development of NADH sensors: glassy carbon electrodes (GCE), multiwalled carbon nanotubes (MWCNT), and carbon black (CB). The GCE and MWCNT has been subjected to oxidative pretreatment to study the influence of oxidative groups for NADH oxidation. The materials had been characterized by FT-IR to identify the surface composition. The response of bare (GC) and GC/modified electrodes toward potassium ferricyanide have been employed to obtain information about the electroactive area and electron transfer rate. Studies of NAD + /NADH redox behavior showed that MWCNT and GCE exhibit high degree of passivation while CB shows no fouling effects. Catalytic effect of surface-oxygenated groups was also proved for GCE and MWCNT, and both, O-GCE and O-MWCNT, exhibited a lower oxidation overpotential compared to the respective untreated materials. Chronoamperometric quantification showed a linear dependence between 2-18 μmol·L −1 and a detection limits of 6.2 μmol·L −1 (GCE), 5.4 μmol·L −1 (O-GCE), 3.2 μmol·L −1 (GCE/ CB), 9.6 μmol·L −1 (GCE/MWCNT), and 4.9 μmol·L −1 (GCE/O-MWCNT) were obtained. The analytical performances suggest that a careful choice of the material for NADH sensing is necessary depending on the sensor application.

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Section: Electrochemical Response For Nadh Using Differentsupporting

confidence: 88%

NADH Oxidation onto Different Carbon-Based Sensors: Effect of Structure and Surface-Oxygenated Groups

et al. 2018

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“…[11][12][13][14][15][16] Furthermore, accumulation of oxidation products involving radical intermediates, such as dimer from the one-electron oxidation product NAD +. , 17,18 can cause deactivation and fouling of the electrode surface.…”

Section: 10mentioning

confidence: 99%

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Jin

Peng

et al. 2015

J. Electrochem. Soc.

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In this study, the composite material that made up polyglycine/3d-4f hybrid-metallic cyano-bridged mixed coordination polymers (CyMCP) was successfully decorated onto a glassy carbon rotating disk electrode (GC RDE) using electrodeposition. The voltammetric behavior of NADH showed typical direct electrooxidation characteristics on the modified GC electrode. Our polyglycine/CyMCP-modified GC RDE was able to measure the apparent heterogeneous electron transfer rate constant of NADH during its direct electrooxidation by preventing fouling effects on the working electrode, which would generally cause a decrease in the oxidative peak current of NADH. In addition, differential pulse voltammetry provided a large linear range between the oxidative peak current and the concentration of NADH from 2-1500 μM at the polyglycine/CyMCP/GC electrode. Finally, the apparent heterogeneous electron transfer rate constant (k s NADH ) of NADH was determined to be 1.73 × 10 −2 cm · s −1 using the polyglycine/CyMCP-modified GC RDE. Both reduced and oxidized forms of β-nicotinamide adenine dinucleotide (NADH and NAD + , respectively) are the coenzymes for a large number of dehydrogenase enzymes (>300) and components of biomarker systems.1 In fact, because of its very important role in the field of analytical electrochemistry, 2,3 bioelectrocatalysis 4-6 and biofuel cells, 7,8 the electrochemical investigation for the formal potential of NADH/NAD + redox couple has attracted much attention. 9,10Therefore, direct oxidation of NADH at untreated or unmodified classical solid electrodes is known to appear poor reversibility and occurs with considerable overpotential (e.g., 1.1 V on carbon and 1.3 V on platinum). [11][12][13][14][15][16] Furthermore, accumulation of oxidation products involving radical intermediates, such as dimer from the one-electron oxidation product NAD +. , 17,18 can cause deactivation and fouling of the electrode surface. Such contamination of the working electrode can largely lower the sensitivity and reproducibility of voltammetric measurements. Consequently, considerable effort has been devoted with a strategy of surface modification on the substrate of conventional materials electrode to attain the electrode's long term stability for determination of NADH at a lower potential. 2,[19][20][21] Over the last few decades, various carbon nanomaterial 22-24 and redox-active mediator materials [25][26][27][28][29][30][31][32][33][34][35] have been modified onto the electrode surface in order to alleviate and/or prevent higher overpotential and fouling problems of the electrode. Therefore, there exist two kinds of catalytic model for the electrooxidation of NADH: one is a direct electro-catalysis 13,15,16,36 and the other is called a redox mediator electro-catalysis. [25][26][27][28][29][30][31][32]37 Though these redox mediators are very efficient electron shuttles for NADH oxidation processes, hydrodynamic voltammetry on the mediator-modified electrode remains a challenge for examining the kinetic parameters of NADH elect...

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“…In the NAD + reduction, the reversible redox process occurs in nicotinamide ring, which accepts two electrons from a substrate in the presence of an appropriated enzyme, forming NADH: SH 2 + NAD + ⇄ S + NADH+ H + [3]. This reaction is particularly important since it allows the identification of non-eletroactive substrates that interact with NAD + and take part in its reduction [8].…”

Section: Introductionmentioning

confidence: 99%

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

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Abstract:A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 µL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD + redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD + signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD + /NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).

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Electrochemical oxidation of NADH at a bare glassy carbon electrode in different supporting electrolytes

Cited by 18 publications

References 42 publications

NADH Oxidation onto Different Carbon-Based Sensors: Effect of Structure and Surface-Oxygenated Groups

NADH Oxidation onto Different Carbon-Based Sensors: Effect of Structure and Surface-Oxygenated Groups

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

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