Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp

Rai, Varun; Deng, Jiajia; Toh, Chee-Seng

doi:10.1016/j.talanta.2012.06.055

Cited by 50 publications

(46 citation statements)

References 36 publications

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“…Due to the surface chemistry of anodic alumina, the pore walls are being functionalized, and numerous sensors are being formed. The detection of glucose oxidase [113], thrombin [55], DNA [211,260,263], surface active agents [123], Ara h1 peanut protein [231], trypsin [222], and urea [281] is enabled. Moreover, application Özt€ urk et al [194] Fig .…”

Section: Applications Of Anodic Aluminum Oxidementioning

confidence: 99%

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

Stępniowski

Bojar

2015

Handbook of Nanoelectrochemistry

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Anodic aluminum oxide (AAO) consists of parallel pores arranged in a honeycomb-like array. Since a two-step self-organized approach in anodic alumina fabrication was invented, tremendous attention was paid to this material. Structural features of anodic alumina, like pore diameter, interpore distance, and nanoporous oxide thickness, can be fully controlled by operating conditions, including type, concentration and temperature of the electrolyte, applied voltage, and duration of the anodization process. Moreover, these operating conditions have also a major impact on the nanoporous array arrangement. Quantitative arrangement analysis methods employing fast Fourier transforms have been developed to assess the nanopores' arrangement. The most frequent application of the anodic aluminum oxide is a template-assisted fabrication of nanostructures. Templates made of the AAO are being successfully applied in fabrication of nanowires, nanotubes, and nanodots by using diversity of techniques, including electrochemical deposition, physical and chemical vapor deposition, sol-gel techniques, etc. Due to the size of obtained nanostructures, magnetic, electric, piezoelectric, luminescent, and catalytic properties of the deposited materials can be significantly improved. Thus, control of the nanopores' structural features and arrangement is crucial for AAO applications in nanotechnology.

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Section: Applications Of Anodic Aluminum Oxidementioning

confidence: 99%

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

Stępniowski

Bojar

2015

Handbook of Nanoelectrochemistry

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“…All electrolyte buffer solutions were purge with nitrogen for 10 min to remove dissolved oxygen prior to the measurement. Configuration of the self assembled layer and immobilization was declared by cyclic voltammetry (CV) in a 0.1 M phosphate buffer saline (pH 7.0) containing 1.0 mM K 3 Fe(CN) 6 at a scan rate of 25 …”

Section: Electrochemical Detectionmentioning

confidence: 99%

“…Electrochemical responses were detected by differential pulse voltammetry under the constant MB at 20 µM in 20 mM Tris buffer saline as it has been suggested that the DPV can remove capacitive currents and known to increase detection limits compared to non-pulsed voltammetry by two fold differences [25] [26]. The plots of overall obtaining reduction peak current of MB are displayed in Figure 4.…”

Section: Electrochemical Responses Of the Complementary Scwl-dna Targmentioning

confidence: 99%

“…A number of DNA based voltammetric detections have been reported for detection of pathogens responsible for diseased states such as the detection of hepatitis B virus [18]- [21], Microcystis spp. [22], HIV [23], Yersinia enterocolitica [24], Legionella pneumophilla [25] dengue virus [26] and Escherichia coli [27]. The detection generally includes the redox labels physically or covalently attached to specific DNA probe or target allocated on the active electrode surface.…”

Section: Introductionmentioning

confidence: 99%

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Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based Voltammetric Electrochemical Determination

Wongkaew¹,

Poosittisak²

2014

AJPS

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A simple and highly sensitive analysis by electrochemical voltammetry has been developed for diagnosis of the most destructive crop disease in Thailand known as sugarcane white leaf (SCWL). Determination of the corresponding DNA interaction has been obtained from the voltammetric signals of electroactive redox methylene blue (MB) by means of cyclic and differential pulse voltammetry. In this study, a chitosan-modified glassy carbon electrode (GCE) was created by self-assembly to produce electrostatic platform for effective immobilization of the DNA. Fabrication of SCWL-DNA hybridization detection system was performed by immobilizing the ssDNA probe as a specific sensor onto chitosan-modified GCE. Hybridization of complementary DNA from the real samples could then be detected by its respective MB signal. This fabricated DNA probe sensor was shown to be capable for discriminative identification among the DNAs from SCWL plants, mosaic virus infected sugarcane and healthy sugarcane plants. Relationship between the specific hybridization signal and DNA target concentration was also observed under optimal condition. The detection limit of 4.709 ng/µl with the regression coefficient (R 2 ) of 0.998 and overall RSD of 2.44% were obtained by response curve fit analysis. The actual SCWL-ssDNA immobilization and hybridizing event were subsequently confirmed by an observation under atomic force microscope. Thus these experiments demonstrate the first successful and effective DNA based voltammetric electrochemical determination for a verification of the specific pathogenic infection within plants from the real epidemic field.

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“…Moreover, at the nanoscale, as the diameter of the nanopore is comparable to the size of biomolecules, it is also possible to observe the effect of the volume exclusion in conductance change. Such a blocking effect can be further amplified by nanoparticle tags attached on target molecules [6,8,14,[19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes

Yang

et al. 2015

Sensors and Actuators B: Chemical

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Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp

Cited by 50 publications

References 36 publications

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

Nanoporous Anodic Aluminum Oxide: Fabrication, Characterization, and Applications

Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based Voltammetric Electrochemical Determination

Impedance sensing of DNA immobilization and hybridization by microfabricated alumina nanopore membranes

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