Electrochemical Nanobiosensor for Glyphosate Herbicide and Its Metabolite

Abstract

The use of amperometric biosensor for the detection of glyphosate herbicide and its metabolite aminomethylphosphonic acid (AMPA) is presented. The biosensor was developed by electrochemically depositing poly(2,5-dimethoxyaniline) (PDMA) doped with poly(4-styrenesulfonic acid) (PSS) onto the surface of a gold electrode followed by electrostatic attachment of horseradish peroxidase (HRP) onto the PDMA-PSS nanocomposite film. The PDMA-PSS film was characterized by transmission electron microscopy (TEM) and Fourier Transform infrared (FTIR) spectroscopy. The HRP immobilized on the PDMA-PSS film catalyzed the reduction of hydrogen peroxide, the inhibition of which was applied in the detection of glyphosate and AMPA. The limits of detection of the biosensor for glyphosate and AMPA were 0.16 mg L À1 and 1.0 mg L À1, respectively. The study demonstrates that the biosensor is very sensitive and could be a useful tool in the screening of glyphosate and AMPA at low concentrations.

Glyphosate and glufosinate detection at electrogenerated NiAl-LDH thin films

“…Obviously, we know that the present analysis method is less sensitive compare to the new amperometric biosensors, published in 2009, and based on the inhibition of horseradish peroxidase activity [4,5]. The limit of detection of this biosensor for Glyp was 0.16 g L −1 , which is very close to EU limit of any pesticides in drinking water (0.1 g L −1 ).…”

“…Indeed, liquid chromatography techniques, using either UV or fluorimetric detectors, are limited to the analysis of pesticides bearing chromophore groups which is not the case with Glyp and Gluf. Electrochemical sensors represent then a good and innovative alternative in term of on-site detection, sensitivity, rapid response, and miniature size for the determination of these pesticides [2][3][4][5].…”

Recent Development on the Electrochemical Detection of Selected Pesticides: A Focused Review

“…Glyphosate has been reported to be detected using bare and modified electrodes made of gold [28,[37][38][39][40][41][42][43][44], copper [45][46][47], carbon [48][49][50][51][52][53][54], mercury [55][56][57], and platinum [58,59]. Electrochemical methods, type of electrodes, analytical performance, measurement condition, and sample matrix for glyphosate determination are summarized in Table 1.…”

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

“…Due to their electrochemical properties, nanomaterials have been applied for electrochemical analysis of many analytes, comprising of nucleic acids [72][73][74], proteins [75,76], secondary metabolites [77,78], and/or metals [79]. The key roles delivered by nanoparticles include biomolecule immobilization, catalysis of electrochemical reactions, enhancement of electron transfer between electrode surfaces and proteins, biomolecule labeling, and even use as a reactant [80].…”