Electrochemical nanobiosensor for express diagnosis of acute myocardial infarction in undiluted plasma

Suprun, Elena V.; Булко, Т. В.; Лисица, А. В.; Gnedenko, O. V.; Ivanov, A. S.; Shumyantseva, Victoria V.; Archakov, Alexander I.

doi:10.1016/j.bios.2009.12.009

Cited by 85 publications

(49 citation statements)

References 36 publications

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“…Myo can be detected in biological fluids such as serum and urine. Myo cut-off values in serum range from 100 to 200 ng mL −1 [9,18,19]. In acute myocardial infarction (AMI) condition, Myo maximum levels are variable; concentrations of 420-600 ng mL −1 have been found in previous studies [18].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical biosensor based on biomimetic material for myoglobin detection

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Dutra

Noronha

et al. 2013

Electrochimica Acta

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a b s t r a c tA novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification.The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 g mL −1 , of positive slope 6.50 ± 1.48 (k mL g −1 ). The limit of detection was 2.25 g mL −1 . Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 g mL −1 . A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 A mL g −1 . MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.

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Section: Introductionmentioning

confidence: 99%

“…Several techniques have been described in the literature for Myo quantification [24][25][26][27][28]. Electro-analytical techniques [9,19,[29][30][31][32][33] have concerned considerable attention due to its sensitive, nondestructive, and rapid electrochemical sensing method.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor based on biomimetic material for myoglobin detection

Moreira

Dutra

Noronha

et al. 2013

Electrochimica Acta

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“…Immunosensors based on CB modified electrodes were prepared according to the principal circuit described in [24,26]. 0.25 mL of 0.1 M DDAB were deposited on the surface of SPE/CB (6 mL); after evaporation of chloroform, the antibodies were immobilized onto the SPE/ DDAB: 0.25 mL of 105 ng/mL anti-cMb solution in PB (pH 6.5) were dropped onto the electrode surface and kept at 4 8C overnight.…”

Section: Preparation Of Electrochemical Immunosensors (Spe/cb/ddab/anmentioning

confidence: 99%

Direct Electrochemistry of Heme Proteins on Electrodes Modified with Didodecyldimethyl Ammonium Bromide and Carbon Black

et al. 2012

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A novel matrix based on commercially available carbon black (CB) N220 and didodecyldimethyl ammonium bromide (DDAB) was shown to be a reliable support for direct electron transfer reactions between screen printed electrode (SPE) and Fe(III)-heme proteins. Cytochrome c (cyt c), myoglobin (Mb), horseradish peroxidase (HRP) and cytochromes P450 (CYP 51A1, CYP 3A4, CYP 2B4) generated well-shaped cyclic voltammograms on SPE/CB/ DDAB electrodes (both in solution and in immobilized state). The attractive performance characteristics of CB modified electrodes are advantageous over single-walled carbon nanotubes (SW CNT) based ones. The achieved direct electrochemistry of heme proteins on CB/DDAB-modified electrodes provided successful elaboration of the immunosensor for cardiac Mb. The immunosensor showed applicability for diagnostics of myocardial infarction displaying significant difference in cardiac Mb content of human blood plasma samples taken from the corresponding patients.

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“…Mb is an oxygen-binding protein used in the transportation and storage of 0 2 in muscle cells [4]. The detection of Mb may be a route to the diagnosis of acute myocardial infarction (AMI), as elevated concentrations of Mb are present in the blood following the onset of the disease [5,6]. Elevated concentrations of Mb (4 -11 nM) are present as early 1-3 hours after AMI [7], which implies that a rapid, accurate and reliable testing method is needed.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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Electrochemistry at liquid-liquid interfaces, or at interfaces between two immiscible electrolyte solutions (ITIES), provides a basis for the non-redox detection of biological molecules, based on iontransfer or adsorption processes. The electroactivity of myoglobin at an array of micron-sized liquidorganogel interfaces was investigated. The µITIES array was patterned with a silicon membrane consisting of an array of eight pores with radii of ~12.8 µm and a pore to pore separation of ~400 µm.Using cyclic voltammetry at the ITIES, the protein was shown to adsorb at the interface and facilitate the transfer of the organic phase electrolyte anions to the aqueous side of the interface. The electrochemical current response was linear with concentration in the range of 1 -6 µM, with corresponding surface coverage of 10 -50 pmol cm -2 . The reverse peak currents was found to be proportional to the voltammetric scan rate, indicating a desorption process. The detection of the protein was only possibly when the pH of the aqueous phase solution was below the pI of the protein. The steady-state simple ion transfer behaviour of tetraethylammonium cation was decreased on the forward sweep, providing a qualitative indication of the presence of adsorbed protein at the interface.Increasing the ionic strength of the aqueous phase resulted in enhanced peak currents, possibly due to aggregation of protein precipitates in the aqueous solution. UV/Vis absorbance spectroscopy was used 1 ISE Member.2 | P a g e to investigate the effects of various aqueous electrolyte solutions on the structure of the protein, and it was shown that at low pH the protein is at least partially denatured. These results provide the basis for label-free detection of myoglobin at the ITIES.

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Electrochemical nanobiosensor for express diagnosis of acute myocardial infarction in undiluted plasma

Cited by 85 publications

References 36 publications

Electrochemical biosensor based on biomimetic material for myoglobin detection

Electrochemical biosensor based on biomimetic material for myoglobin detection

Direct Electrochemistry of Heme Proteins on Electrodes Modified with Didodecyldimethyl Ammonium Bromide and Carbon Black

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

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