Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things

Abstract

An Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD’s limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L−1/mm2 respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD’s response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.

Microfluidic sensors based on two-dimensional materials for chemical and biological assessments

“…This versatile platform allows for rapid analysis, integrated processing, high portability and analysis of complex biological fluids with high sensitivity, efficiency and multiplexed detection. Among these advantages, microfluidics offers a unique chance for MOFs in controlled synthesis and sensing applications 175,176 . including resource-limited settings and POC diagnostics [177][178][179] .…”

“…Among these advantages, microfluidics offers a unique chance for MOFs in controlled synthesis and sensing applications. 175,176 5.1 MOF-based microfluidic bisensors 5.1.1 For biomolecules detection. Impressively, most of the MOF limitations are the advantages of microfluidic systems.…”

Fluorescence-Based Sensing of Pesticides Using Supramolecular Chemistry

“…In the same year, researchers reported that a biphenyl-containing MOF could bind low concentrations of organophosphate pesticides, with the success of such binding measured using electrochemical analysis (Wen et al, 2010). A variety of other electrochemical-based detections of pesticide binding in MOFs have since been reported (Nagabooshanam et al, 2019;Tu et al, 2019;Chansi et al, 2020).…”

A Review on Bio-Cyber Interfaces for Intrabody Molecular Communications Systems

“…Healthcare platforms, such as point-of-care diagnostics, have been built using nanoscale biosensors to enable a faster detection of diseases [11]. Often, these devices are composed by electrochemical biosensors that would detect chemical signals (e.g., biomolecules) and convert them into electrical signals, that can be stored in the platform's memory and also displayed as the result of the chemical measurement [12,13].…”