Electrochemical Methods for Assessment of Polytrauma Outcomes

Goroncharovskaya, I. V.; Евсеев, А. К.; Шабанов, А. К.; Denisenko, Oleg; Kuzovlev, Аrtem; Klychnikova, E. V.; Tazina, E. V.; Петриков, С. С.

doi:10.1002/elan.202060356

Cited by 3 publications

(3 citation statements)

References 43 publications

(37 reference statements)

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“…Определение уровня МДА в сыворотке пациентов проводили с применением тиобарбитуровой кислоты [31]. Состояние антиоксидантной системы оценивали по показателю об щей антиокислительной активности (ОАА) сыворотки крови, которую измеряли спектрофотометрическим методом на биохимическом анализаторе Olympus AU2700 (Beckman Coulter, США) с использованием набора реактивов TAS kit (Randox, Великобритания), а также электрохимическим методом по величине суммарного количества электричества (Q), затраченного на окисление всех низкомолекулярных антиоксидантов [31]. Измерение ПРЦ платинового электрода в плазме крови проводили на потенциостате IPC Compact (ООО «НТФ «Вольта», Россия).…”

Section: материалы и методыunclassified

“…На 1-е сутки отмечается снижение концентрации АПФ: 23,68 (21,19) ммоль/л против 29,25 (24,25) ммоль/л в группе Даларгина (р = 0,0328). Учитывая значимую роль АПФ в регуляции тонуса сосудов наряду с оксидом азота (NO), данный показатель может быть признаком развития эндотелиальной дисфункции на фоне массивного травматического повреждения и ишемии-реперфузии органов и тка ней [31].…”

Section: рис 1 распределение групп по механизму получения травмыunclassified

“…При развитии окислительного стресса отмечается выраженная гиперпродукция окисленных липопротеинов низкой плотности (Oxidized low-density lipoprotein -ox-LDL), конечным продуктом метаболизма которых является МДА [32]. При этом в ряде исследований ox-LDL связывают с развитием эндотелиальной дисфункции [31,37]. В то же время в функционировании эндотелиальных клеток одну из важных ро лей играет транскрипционный фактор Nrf2 (ядерный фактор транскрипции НРФ-2, англ.…”

Section: рис 1 распределение групп по механизму получения травмыunclassified

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The effect of a tyrosyl-D-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate (Dalargin) on oxidative stress in patients with severe combined trauma: a prospective clinical study

Antonova,

Evseev,

Goroncharovskaya

et al. 2023

Annals of Critical Care

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INTRODUCTION: Severe combined trauma remains the leading cause of mortality and disability of the workable population. Under damaging factor influence, a whole cascade of pathological processes is triggered, leading to development of multiple organ failure. OBJECTIVE: Exploration of the effects synthetic analogue of tyrosyl-D-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate (Dalargin) on the dynamics of oxidative stress markers in patients with severe polytrauma and the development of organ dysfunction. MATERIALS AND METHODS: 104 patients with severe combined trauma were included in study. There were 38 patients in the Dalargin group and 66 patients in the control group. Patients from main group received Dalargin in the form of a constant infusion through a syringe dispenser at the dose of 10 mcg/kg/hour at first 12 hours of hospitalization and 5 mcg/kg/hour up to 72 hours from the moment of admission. The control group received standard treatment. Indicators of oxidative stress were evaluated at the time of admission (0 days), 1, 3, 5, 7, 10 and 14 days from the therapy onset. RESULTS: In the main group, there was a significant decrease in oxidative stress markers such as malondialdehyde from the first day of treatment (p < 0.05), normalization of stable nitric oxide metabolites at day 10, and a decrease in total antioxidant activity below normal throughout the observation period. Mortality was comparable in both groups (p > 0.05), but the length of hospital stay was lower in surviving patients in the Dalargin group (p < 0.05). The development of organ dysfunction (acute respiratory distress syndrome, acute kidney injury) was less frequent in the Dalargine group (p < 0.05), but infectious complications (pneumonia, meningitis, wound suppuration) were comparable. CONCLUSIONS: Dalargin leads to a decrease of oxidative stress markers and normalization of endothelial function indicators, which contributes to a decrease of organ dysfunction frequency.

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Section: материалы и методыunclassified

Section: рис 1 распределение групп по механизму получения травмыunclassified

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The effect of a tyrosyl-D-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate (Dalargin) on oxidative stress in patients with severe combined trauma: a prospective clinical study

Antonova,

Evseev,

Goroncharovskaya

et al. 2023

Annals of Critical Care

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Degradation Modeling and Lifetime Evaluation for Organic Anti-Corrosion Coatings Using a Three-Stage Electrochemical Statistical Model

Ji,

Ma,

Cai

et al. 2023

J Polym Environ

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Electrochemical analysis of antioxidant status of biological media in different sampling and storage conditions

Goroncharovskaya,

Evseev,

Shabanov

et al. 2024

JSCS

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The use of an electrochemical approach for assessment of the oxidative stress severity is a promising direction for point-of-care testing development, which is especially important for critically ill patients. The aim of this study was to determine the influence of different types of blood collection tubes (with clot activator and separating gel, lithium heparin, sodium citrate and K2EDTA) and storage conditions (at +25?C, +4?C and -23?C up to 5 days) on the electrochemical analysis of the antioxidant status of blood plasma, assessed by measuring the open circuit potential of platinum electrode (OCP, mV) and antioxidant capacity via cyclic voltammetry method (q, ?C). It was obtained that blood collection tubes with lithium heparin and clot activator are the most suitable for the electrochemical analysis of antioxidant status of blood plasma, since they do not affect the results of measurements. Furthermore, data obtained during storage blood plasma samples in different temperature conditions indicate that it is preferable to perform electrochemical analysis in fresh samples.

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Electrochemical Methods for Assessment of Polytrauma Outcomes

Cited by 3 publications

References 43 publications

The effect of a tyrosyl-D-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate (Dalargin) on oxidative stress in patients with severe combined trauma: a prospective clinical study

The effect of a tyrosyl-D-alanyl-glycyl-phenylalanyl-leucyl-arginine diacetate (Dalargin) on oxidative stress in patients with severe combined trauma: a prospective clinical study

Degradation Modeling and Lifetime Evaluation for Organic Anti-Corrosion Coatings Using a Three-Stage Electrochemical Statistical Model

Electrochemical analysis of antioxidant status of biological media in different sampling and storage conditions

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