Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein

Sinawang, Prima Dewi; Rai, Varun; Ionescu, Rodica Elena; Marks, Robert S.

doi:10.1016/j.bios.2015.09.048

Cited by 130 publications

(75 citation statements)

References 58 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More recently, Sinawang et al [119] described an electrochemical lateral flow immunosensor for detection of dengue NS1 protein. A conventional lateral flow architecture was adapted to a sandwich-type electrochemical transduction provided by a AuNP-labeled NS1 antibody, with Fc grafted on the AuNPs.…”

Section: Discussionmentioning

confidence: 99%

“…Copyright © 2012 WILEY-VCH Verlag GmbH & Co; ( D ) Scheme of the electrochemical lateral flow immunosensor for dengue detection, with AuNPs/Fc-labeled antibodies; ( E ) CVs of NS1 protein detection over the lateral flow immunosensor, for concentrations from 0.5 ng mL −1 to 400 ng mL −1 of NS1 protein. Reprinted from [119], Copyright 2016, with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

View full text Add to dashboard Cite

Immunosensors have experienced a very significant growth in recent years, driven by the need for fast, sensitive, portable and easy-to-use devices to detect biomarkers for clinical diagnosis or to monitor organic pollutants in natural or industrial environments. Advances in the field of signal amplification using enzymatic reactions, nanomaterials such as carbon nanotubes, graphene and graphene derivatives, metallic nanoparticles (gold, silver, various oxides or metal complexes), or magnetic beads show how it is possible to improve collection, binding or transduction performances and reach the requirements for realistic clinical diagnostic or environmental control. This review presents these most recent advances; it focuses first on classical electrode substrates, then moves to carbon-based nanostructured ones including carbon nanotubes, graphene and other carbon materials, metal or metal-oxide nanoparticles, magnetic nanoparticles, dendrimers and, to finish, explore the use of ionic liquids. Analytical performances are systematically covered and compared, depending on the detection principle, but also from a chronological perspective, from 2012 to 2016 and early 2017.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

View full text Add to dashboard Cite

show abstract

“…Prima DewiSinawang et al . constructed an electrochemical lateral flow immunosensor connected to a screen‐printed gold electrode for the detection of NS1 nonstructural protein, known to be related to dengue (Figure C).…”

Section: Applications Of Paper Devicesmentioning

confidence: 97%

Emerging Considerations for the Future Development of Electrochemical Paper‐Based Analytical Devices

et al. 2018

View full text Add to dashboard Cite

Meeting the current needs for easier, more precise and faster analyses that also follow the principles of green analytical chemistry requires novel analytical chemistry strategies. Since the appearance in this century of the first device based on a paper platform, many studies have been presented in the literature, providing a wide range of designs and possibilities for the application of paper platforms to electroanalytical systems. This Review gives an overview of the field and can pave the way for the future development of electrochemical paper‐based analytical devices. We also present a critical point of view regarding what has been investigated and developed and what is still missing. This Review discusses the efforts made in the field related to important topics such as the choice of the paper substrate, the device construction process, the characterization of the device, and applications in different areas. In this way, we indicate some steps necessary for optimizing the design of the devices, with a focus on multidisciplinary collaborations that could move entire systems from the bench of the laboratory to the field.

show abstract

“…A paper‐based chemiluminescence (CL) analytical device (Liu, Kou, Xing, & Li, ) was developed for the first time, combined with paper chromatography for the determination of dichlorvos (DDV) in vegetables without complicated sample pretreatment. A cellulose glassy fiber paper (Sinawang, Rai, Ionescu, & Marks, ) with an Electrochemical Lateral Flow Immunosensor (ELFI) were used to combine with screen‐printed gold electrodes (SPGE), enabling quantification together with the convenience of a lateral flow test strip. AuNPs‐embedded paper‐based lab‐in‐a‐syringe (LIS) device (Zor & Bekar, ) was developed as a sensing strategy.…”

Section: Introductionmentioning

confidence: 99%

A portable detection method for organophosphorus and carbamates pesticide residues based on multilayer paper chip

Yang

Wang

Xue

et al. 2018

J Food Process Engineering

View full text Add to dashboard Cite

Organophosphorus and carbamates pesticide residues detection is one of significance in vegetables and fruits production. However, an effective directly detection method without any auxiliary has not been reported yet. Herein, we proposed a multilayer paper chip, including injection layer, heating layer, conduction layer, fixed enzyme layer, fixed indophenol acetate layer, and color layer, for pesticide residue of organophosphorus and carbamates detection based on the principles of enzyme inhibition reaction and heat generation inside. It's the first time for paper‐based chip to include an inside built‐in heating layer, which can ensure the reaction process with enzyme is always at the optimum temperature. Without any external instrument, we can determine whether the pesticide exceeds the standard according to the reaction color. Besides, we can also make quantitative detection of pesticide with external spectrometer detection system. The overall detection process runs automatically, then relatively accurate test results can be acquired. Under the optimum condition, a good linear correlation for dipterex concentrations over the range from 0.05 to 0.5 mg/L was obtained by quantitative detection, which can reach a detection limit (LOD) of 0.0406 mg/L. Besides, it also showed satisfactory reproducibility, specificity, and stability. Therefore, it is suitable for the instant detection technique. Practical applications This article provides a method which is used to detect organophosphorus and carbamates pesticide residues existing in vegetables and fruits production. The detection limit is 0.0406 mg/L and it is lower than national standards. The detection reproducibility is acceptable. This method is energy‐saving and environment friendly. In this article, multilayer paper chip that includes enzyme inhibition reaction, color reaction and exothermic reaction was proposed. The heating layer in multilayer paper chip relies on the principle of chemical heat production and needs only a little water, which is very simple and easy. Under the whole process of detection without manual intervention, the testing result from the paper chip not only can be distinguished by eyes, but also can be detected precisely by simple detection device, which provide a basis for development of portable pesticide detector.

show abstract

Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein

Cited by 130 publications

References 58 publications

Recent Advances in Electrochemical Immunosensors

Recent Advances in Electrochemical Immunosensors

Emerging Considerations for the Future Development of Electrochemical Paper‐Based Analytical Devices

A portable detection method for organophosphorus and carbamates pesticide residues based on multilayer paper chip

Contact Info

Product

Resources

About