Electrochemical investigation of increased carbon steel corrosion via extracellular electron transfer by a sulfate reducing bacterium under carbon source starvation

Dou, Wenwen; Liu, Jialin; Cai, Wenjun; Wang, Di; Jia, Ru; Chen, Shougang; Gu, Tingyue

doi:10.1016/j.corsci.2019.02.005

Cited by 125 publications

(41 citation statements)

References 53 publications

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“…Environments with an abundant supply of nutrients would be expected to favour attachment and biofilm formation, however, previous studies have shown a greater tendency to form biofilms under low-nutrients or starvation conditions on October 11, 2020 by guest http://aem.asm.org/ Downloaded from 6 (18). Likewise, in Electrical-MIC, microorganisms have been shown to switch from soluble electron donors to metallic iron as the only energy source to carry out microbial activities under starving conditions (19). This mechanism has been shown to lead to higher corrosion rates in the laboratory (20).…”

Section: Downloaded Frommentioning

confidence: 98%

“…However, under these conditions biofilms are known to operate under stress and are unable to multiply because metal does not provide the carbon that cells need for growth (20). Although under starvation cells can scavenge nutrients from the EPS and dead cells, reduction in the cell counts are frequently observed when carbon sources are depleted (19,21). In field, MIC has been mainly associated with low velocity or infrequently flooded systems, deadlegs and pipelines with solid deposit accumulation that cannot be adequately cleaned.…”

Section: Downloaded Frommentioning

confidence: 99%

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Nutrient Level Determines Biofilm Characteristics and Subsequent Impact on Microbial Corrosion and Biocide Effectiveness

Salgar-Chaparro

Lepková

Pojtanabuntoeng

et al. 2020

Appl Environ Microbiol

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The impact that nutrient level has on biofilm characteristics, biocide effectiveness, and the associated risk of microbiologically influenced corrosion (MIC) was assessed using multispecies biofilms from two different oilfield consortia. A range of microbiological, microscopy, and corrosion methods demonstrated that the continuous flow of nutrients for the microbial growth resulted in higher activity, thickness, and robustness of the biofilms formed on carbon steel, which induced greater localized corrosion compared to biofilms formed under batch, nutrient-depleted conditions. Despite of the differences in biofilm characteristics, biofilms displayed comparable susceptibilities to glutaraldehyde biocide, with similar log10 reductions and percent reductions of microorganisms under both nutrient conditions. Nevertheless, nutrient replenishment impacted the effectiveness of the biocide in controlling microbial populations; a higher concentration of cells survived the biocide treatment in biofilms formed under a continuous flow of nutrients. Complementary DNA-/RNA-based amplicon sequencing and bioinformatics analysis were used to discriminate the active within the total populations in biofilms established at the different nutrient conditions and allowed the identification of the microbial species that remained active despite nutrient depletion and biocide treatment. Detection of persistent active microorganisms after exposure to glutaraldehyde, regardless of biofilm structure, suggested the presence of microorganisms less susceptible to this biocide and highlighted the importance of monitoring active microbial species for the early detection of biocide resistance in oil production facilities. IMPORTANCE Microbiologically influenced corrosion (MIC) is a complex process that generates economic losses to the industry every year. Corrosion must be managed to prevent a loss of containment of produced fluids to the external environment. MIC management includes the identification of assets with higher MIC risk, which could be influenced by nutrient levels in the system. Assessing biofilms under different nutrient conditions is essential for understanding the impact of flow regime on microbial communities and the subsequent impact on microbial corrosion and on the effectiveness of biocide treatment. This investigation simulates closely oil production systems, which contain piping sections exposed to continuous flow and sections that remain stagnant for long periods. Therefore, the results reported here are useful for MIC management and prevention. Moreover, the complementary methodological approach applied in this investigation highlighted the importance of implementing RNA-based methods for better identification of active microorganisms that survive stress conditions in oil systems.

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Section: Downloaded Frommentioning

confidence: 98%

Section: Downloaded Frommentioning

confidence: 99%

Nutrient Level Determines Biofilm Characteristics and Subsequent Impact on Microbial Corrosion and Biocide Effectiveness

Salgar-Chaparro

Lepková

Pojtanabuntoeng

et al. 2020

Appl Environ Microbiol

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“…Thus, it is not surprising that pre-grown mature SRB biofilms corrode more aggressively when exposed to a fresh culture medium with no or a reduced level of carbon source (Dou et al, 2019). A hot water tank provides a breeding ground for bacteria.…”

Section: Examples Of Biocorrosion Against Various Materials In Different Settingsmentioning

confidence: 99%

Biocorrosion caused by microbial biofilms is ubiquitous around us

Dou

2020

Microbial Biotechnology

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Biocorrosion first surfaced in the scientific literature when Richard H. Gaines associated corrosion with bacterial activities in 1910. It is also known as microbiologically influenced corrosion (MIC). In general, it covers two scenarios. One is that microbes cause corrosion directly, which usually means microbes secrete corrosive metabolites or microbes harvest electrons from a metal for respiration to produce energy. In the second scenario, microbes are behind the initiation or acceleration of corrosion caused by a pre-existing corrosive agent such as water and CO 2 , by compromising the passive film (often a metal oxide film on a metal). MIC is caused by microbial biofilms. It is everywhere around us. This work dissects some notable examples with perspectives.

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“…The SRB are anaerobic bacteria that use sulfate as the terminal electron acceptor in their respiration (Dannenberg et al, 1992;Heidelberg et al, 2004;Lv and Du, 2018). When lactate is used as the organic carbon source, the following oxidation reaction occurs in the cytoplasm of the SRB under enzyme catalysis (Xu and Gu, 2011;Xu and Gu, 2014;Xu et al, 2016;Li et al, 2018;Dou et al, 2019;Gu et al, 2019): where the apostrophe in the reduction potential E °' of the two reactions indicates a pH of 7.0. When there is a lack of carbon source in the local environment such as at the bottom of the sulfate-reducing bacterial biofilm, elemental iron can provide the electrons for the SRB survival through extracellular Fe oxidation, as shown in Eq.…”

Section: Introductionmentioning

confidence: 99%

Carbon Source Starvation of a Sulfate-Reducing Bacterium–Elevated MIC Deterioration of Tensile Strength and Strain of X80 Pipeline Steel

et al. 2021

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It is known that starved sulfate-reducing bacterial biofilms corrode carbon steel more aggressively because they use electrons from elemental iron oxidation as an alternative source of energy. This work used carbon source starvation to vary MIC (microbiologically influenced corrosion) severity for studying subsequent MIC impacts on the degradation of X80 carbon steel mechanical properties. X80 square coupons and dogbone coupons were immersed in ATCC 1249 culture medium (200 ml in 450-ml anaerobic bottles) inoculated with Desulfovibrio vulgaris for 3-day pre-growth and then for an additional 14 days in fresh media with adjusted carbon source levels for starvation testing. After the starvation test, the sessile cell counts (cells/cm2) on the dogbone coupons in the bottles with carbon source levels of 0, 10, 50, and 100% (vs that in the full-strength medium) were 8.1 × 106, 3.2 × 107, 8.3 × 107, and 1.3 × 108, respectively. The pit depths from the X80 dogbone coupons were 1.9 μm (0%), 4.9 μm (10%), 9.1 μm (50%), and 6.4 μm (100%). The corresponding weight losses (mg/cm2) from the square coupons were 1.9 (0%), 3.3 (10%), 4.4 (50%), and 3.7 (100%). The 50% carbon source level had the combination of carbon starvation without suffering too much sessile cell loss. Thus, both its pit depth and weight loss were the highest. The electrochemical tests corroborated the pit depth and weight loss trends. The tensile tests of the dogbone coupons after the starvation incubation indicated that sulfate-reducing bacteria (SRB) made X80 more brittle and weaker. Compared with the fresh (no-SRB-exposure) X80 dogbone coupon’s ultimate tensile strain of 13.6% and ultimate tensile stress of 860 MPa, the 50% carbon source level led to the lowest ultimate tensile strain of 10.3% (24% loss when compared with the fresh dogbone) and ultimate tensile stress of 672 MPa (22% loss). The 100% carbon source level had a smaller loss in ultimate tensile strain than the 50% carbon source level, followed by 10% and then 0%. Moreover, the 100% carbon source level had a smaller loss in ultimate tensile strength than the 50%, followed by 10% and 0% in a tie. This outcome shows that even in the 17-day short-term test, significant degradation of the mechanical properties occurred and more severe MIC pitting caused more severe degradation.

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Electrochemical investigation of increased carbon steel corrosion via extracellular electron transfer by a sulfate reducing bacterium under carbon source starvation

Cited by 125 publications

References 53 publications

Nutrient Level Determines Biofilm Characteristics and Subsequent Impact on Microbial Corrosion and Biocide Effectiveness

Nutrient Level Determines Biofilm Characteristics and Subsequent Impact on Microbial Corrosion and Biocide Effectiveness

Biocorrosion caused by microbial biofilms is ubiquitous around us

Carbon Source Starvation of a Sulfate-Reducing Bacterium–Elevated MIC Deterioration of Tensile Strength and Strain of X80 Pipeline Steel

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