Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes
Adriána Dusíková,
Timea Baranová,
Ján Krahulec
et al.
Abstract:The study presents a promising approach to enzymatic kinetics using Electrochemical Impedance Spectroscopy (EIS) to assess fundamental parameters of modified enteropeptidases. Traditional methods for determining these parameters, while effective, often lack versatility and convenience, especially under varying environmental conditions. The use of EIS provides a novel approach that overcomes these limitations. The enteropeptidase underwent genetic modification through the introduction of single amino acid modif… Show more
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