Electrochemical impedance biosensor for detection of saxitoxin in aqueous solution

Serrano, Pablo G.; Nunes, Gisele E.; Junior, Lindiomar Borges de Avila; Reis, Carleane P.S.; Gomes, Aldo M.C.; Reis, Françoise T.; Sartorelli, M. L.; Melegari, Sílvia Pedroso; Matias, William Gerson; Bechtold, Ivan H.

doi:10.1007/s00216-021-03603-1

Cited by 24 publications

(19 citation statements)

References 42 publications

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“…Electrochemical impedance spectroscopy (EIS) can be used to evaluate changes of R ct when target analytes are captured by the electrode material through supramolecular interactions such as hydrogen bonding between them. 96 After the addition of NO 2 − in 0.1 M H 2 SO 4 , a smaller R ct at the interface between the surface of the Mn-CP electrode and the electrolyte was observed, confirming the intermediate formation of [Mn-CP• NO 2 − ] upon NO 2 − recognition (Figure 8A). To illustrate the effect of the operating potential on the current response of electrochemical nitrite sensing, the ampere responses of Mn-CP/GCE after successive increments of NaNO 2 in the continuously stirred 0.1 M H 2 SO 4 every 30 s were measured through three parallel experiments (+0.90, +0.95, and +1.00 V), respectively (Figure S9).…”

Section: Electrochemical Sensing Performance 341 Detection Of Nitrite...supporting

confidence: 52%

Proton-Conductive and Electrochemical-Sensitive Sensing Behavior of a New Mn(II) Chain Coordination Polymer

Yang

Zhang

2023

Crystal Growth & Design

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In this work, a novel Mn(II) chain coordination polymer (Mn-CP), {[Mn(iip)(2bbpum) 0.5 (H 2 O) 3 ]•2H 2 O} n [H 2 iip = 5-iodoisophthalic acid, 2bbpum = N,N′-bis(2-pyridinemethyl)oxamide] was synthesized under ambient conditions. Mn-CP further assembles into a 3D supramolecular architecture through hydrogen bonds, halogen bonds, and ππ stacking interactions. The composite Mn-CP/nafion membrane has low activation energy for proton transfer, which results in its temperature-insensitive proton conductivity. The cyclic voltammetry demonstrates that the Mn-CP electrode (Mn-CP/GCE) shows quite different redox properties in 0.1 M H 2 SO 4 and 0.1 M PBS aqueous solutions. The Mn-CP/GCE exhibits a couple of irreversible redox peaks with an oxidation peak potential suitable for the nitrite oxidation in 0.1 M H 2 SO 4 . Quite differently, in the PBS aqueous buffer solution, the Mn-CP electrode has a couple of irreversible redox peaks. The reduction peak potential is appropriate for the H 2 O 2 reduction. So, the Mn-CP/GCE can be used not only in the electrocatalytic oxidation of NO 2 − but also in the electrocatalytic reduction of H 2 O 2 . The amperometric response reveals that the Mn-CP/GCE exhibits high-selective and extremely sensitive oxidation sensing of NO 2 − and reduction sensing of H 2 O 2 . The exponential detection range is 0.01−20 mM for NO 2 − and 0.01−10.5 mM for H 2 O 2 . The linear detection range for both H 2 O 2 and NO 2 − is 0.01−0.10 mM. The detection limit is 1.865 μM for NO 2 − and 8.57 μM for H 2 O 2 . The Mn-CP/GCE exhibits high electrochemical stability and fine reproducibility. Moreover, the strategy can be translated to a portable screen-printed electrode (SPE). The Mn-CP/SPE shows more sensitive sensing to NO 2 − and H 2 O 2 than Mn-CP/GCE. Moreover, it can detect NO 2 − and H 2 O 2 efficiently in the real-world samples. So, Mn-CP may be a potential dual nonenzymatic electrochemical sensory material for NO 2 − via oxidation sensing and H 2 O 2 via reduction sensing.

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Section: Electrochemical Sensing Performance 341 Detection Of Nitrite...supporting

confidence: 52%

Proton-Conductive and Electrochemical-Sensitive Sensing Behavior of a New Mn(II) Chain Coordination Polymer

Yang

Zhang

2023

Crystal Growth & Design

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“…Next, EIS spectra were recorded to study the surface properties of the sensor, as quantified by the charge transfer resistance, R ct , since this is the most sensitive parameter to characterize what is happening on the electrode surface [35,37]. To obtain the R ct values, the Nyquist plot was fitted with a Randles equivalent circuit model (inset of Fig.…”

Section: Sensor Surface Characterizationmentioning

confidence: 99%

Point-of-care human milk testing for maternal secretor status

2021

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We present an electrochemical impedimetric-based biosensor for monitoring the variation in human milk oligosaccharide (HMO) composition. 2′-Fucosyllactose (2’FL) is an HMO associated with infant growth, cognitive development, and protection from infectious diarrhea, one of the major causes of infant death worldwide. Due to genetic variation, the milk of some women (non-secretors) contains no or very little 2′FL with potential implications for infant health and development. However, there is currently no technology to analyze the presence and concentration of HMOs in human milk at the point-of-care (POC). The lack of such technology represents a major impediment to advancing human milk research and improving maternal-infant health. Towards this unmet need, we report an impedimetric assay for HMOs with an α-1,2 linkage, the most abundant of which is 2′FL. The sensor uses a lectin for affinity, specifically Ulex europaeusagglutininI (UEA), with electrochemical readout. In spiked studies, the sensor exhibited a high degree of linearity (R2 = 0.991) over 0.5 to 3.0 μM with a 330-nM detection limit. The sensor performance was clinically validated using banked human milk samples and correctly identified all secretor vs. non-secretor samples. Furthermore, despite the short 35-min assay time and low sample volume (25 μL), the assay was highly correlated with HPLC measurements. This bedside human milk testing assay enables POC, “sample-to-answer” quantitative HMO measurement, and will be a valuable tool to assess milk composition. Graphical abstract

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“… 8 The desired features of biosensors are stability, affordability, sensitivity, and accuracy. 9 , 10 Biosensors detect analytes in the form of electrical, thermal, or optical signals mediated by enzymes or antibodies (Abs). 11 , 12 These devices have a plethora of applications ranging from clinical to environmental.…”

Section: Introductionmentioning

confidence: 99%

“…They established their importance by detecting cancer in early stages, checking diabetes, confirming pregnancy, detecting highly transmissible virulent viruses, and many more. , Biosensor-based devices are composed of bioentities, and the detection mechanism for the bioentities has been represented schematically (Figure ). The desired features of biosensors are stability, affordability, sensitivity, and accuracy. , Biosensors detect analytes in the form of electrical, thermal, or optical signals mediated by enzymes or antibodies (Abs). , These devices have a plethora of applications ranging from clinical to environmental.…”

Section: Introductionmentioning

confidence: 99%

Prospects of Biosensors Based on Functionalized and Nanostructured Solitary Materials: Detection of Viral Infections and Other Risks

et al. 2022

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Advances in nanotechnology over the past decade have emerged as a substitute for conventional therapies and have facilitated the development of economically viable biosensors. Next-generation biosensors can play a significant role in curbing the spread of various viruses, including HCoV-2, and controlling morbidity and mortality. Pertaining to the impact of the current pandemic, there is a need for point-of-care biosensor-based testing as a detection method to accelerate the detection process. Integrating biosensors with nanostructures could be a substitute for ultrasensitive label-free biosensors to amplify sensing and miniaturization. Notably, next-generation biosensors could expedite the detection process. An elaborate description of various types of functionalized nanomaterials and their synthetic aspects is presented. The utility of the functionalized nanostructured materials for fabricating nanobiosensors to detect several types of viral infections is described in this review. This review also discusses the choice of appropriate nanomaterials, as well as challenges and opportunities in the field of nanobiosensors.

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Electrochemical impedance biosensor for detection of saxitoxin in aqueous solution

Cited by 24 publications

References 42 publications

Proton-Conductive and Electrochemical-Sensitive Sensing Behavior of a New Mn(II) Chain Coordination Polymer

Proton-Conductive and Electrochemical-Sensitive Sensing Behavior of a New Mn(II) Chain Coordination Polymer

Point-of-care human milk testing for maternal secretor status

Prospects of Biosensors Based on Functionalized and Nanostructured Solitary Materials: Detection of Viral Infections and Other Risks

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