Electrochemical Immunosensor for the Sensitive Detection of Alzheimer's Biomarker Amyloid‐β (1–42) Using the Heme‐amyloid‐β (1–42) Complex as the Signal Source

Ding, Minling; Shu, Qi; Zhang, Nan; Yan, Chaoren; Niu, Huizhe; Li, Xiaoqian; Guan, Ping; Hu, Xiaoling

doi:10.1002/elan.202100392

Cited by 9 publications

(5 citation statements)

References 78 publications

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“…Alzheimer's disease is typically only identified after the commencement of the disease, so there is an urgent need to create diagnostic techniques for early disease markers. Ding et al produced an immunosensor for the detection of Aβ 42 peptides depending on the heme covalently bound to Aβ 42 possessing peroxidase-like activity [13]. Fc was chosen as signal indicator, as seen in Figure 2.…”

Section: Redox-couple-labeled Electrochemical Immunosensorsmentioning

confidence: 99%

“…The constructed device has a detection range of 1:1000-1:200 v/v for SARS-CoV-2. [13]. Copyright (2021) John Wiley.…”

Section: Horseradish Peroxidasementioning

confidence: 99%

“…Alzheimer's disease is a typical neurological disease of the elderly and is not curable [12]. The β protein and the P53 protein in peripheral blood and cerebrospinal fluid are the biomarkers for the clinical beginning of Alzheimer's disease [13,14]. Additionally, procalcitonin is the marker for septicemia.…”

Section: Introductionmentioning

confidence: 99%

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The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

et al. 2023

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Disease-related biomarkers may serve as indicators of human disease. The clinical diagnosis of diseases may largely benefit from timely and accurate detection of biomarkers, which has been the subject of extensive investigations. Due to the specificity of antibody and antigen recognition, electrochemical immunosensors can accurately detect multiple disease biomarkers, including proteins, antigens, and enzymes. This review deals with the fundamentals and types of electrochemical immunosensors. The electrochemical immunosensors are developed using three different catalysts: redox couples, typical biological enzymes, and nanomimetic enzymes. This review also focuses on the applications of those immunosensors in the detection of cancer, Alzheimer’s disease, novel coronavirus pneumonia and other diseases. Finally, the future trends in electrochemical immunosensors are addressed in terms of achieving lower detection limits, improving electrode modification capabilities and developing composite functional materials.

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Section: Redox-couple-labeled Electrochemical Immunosensorsmentioning

confidence: 99%

“…The constructed device has a detection range of 1:1000-1:200 v/v for SARS-CoV-2. [13]. Copyright (2021) John Wiley.…”

Section: Horseradish Peroxidasementioning

confidence: 99%

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The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

et al. 2023

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“…Ding et al, 2021, reported an Aβ immunosensor using a heme-Aβ (1-42) conjugated structure as a signal amplifier to detect the analyte in serum and saliva [76]. A GCE (diameter: 3 mm) was activated using CV in 0.5 M H 2 SO 4 under potential from −0.5 to 1.6 V. Then, the electrode surface was modified with AuNPs through the electrodeposition of a HAuCl 4 solution via the CV technique.…”

Section: Electrochemical Immunosensing Of Aβmentioning

confidence: 99%

Electrochemical Immunosensors Developed for Amyloid-Beta and Tau Proteins, Leading Biomarkers of Alzheimer’s Disease

et al. 2023

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Alzheimer’s disease (AD) is the most common neurological disease and a serious cause of dementia, which constitutes a threat to human health. The clinical evidence has found that extracellular amyloid-beta peptides (Aβ), phosphorylated tau (p-tau), and intracellular tau proteins, which are derived from the amyloid precursor protein (APP), are the leading biomarkers for accurate and early diagnosis of AD due to their central role in disease pathology, their correlation with disease progression, their diagnostic value, and their implications for therapeutic interventions. Their detection and monitoring contribute significantly to understanding AD and advancing clinical care. Available diagnostic techniques, including magnetic resonance imaging (MRI) and positron emission tomography (PET), are mainly used to validate AD diagnosis. However, these methods are expensive, yield results that are difficult to interpret, and have common side effects such as headaches, nausea, and vomiting. Therefore, researchers have focused on developing cost-effective, portable, and point-of-care alternative diagnostic devices to detect specific biomarkers in cerebrospinal fluid (CSF) and other biofluids. In this review, we summarized the recent progress in developing electrochemical immunosensors for detecting AD biomarkers (Aβ and p-tau protein) and their subtypes (AβO, Aβ(1-40), Aβ(1-42), t-tau, cleaved-tau (c-tau), p-tau181, p-tau231, p-tau381, and p-tau441). We also evaluated the key characteristics and electrochemical performance of developed immunosensing platforms, including signal interfaces, nanomaterials or other signal amplifiers, biofunctionalization methods, and even primary electrochemical sensing performances (i.e., sensitivity, linear detection range, the limit of detection (LOD), and clinical application).

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“…Depending on the requirements, available equipment, and labor ability, each technique can be applied reasonably and effectively. Moreover, Aβ peptides can be detected using labels with enzymes, nanozymes, or anti-IgG-ALP, [ 86 , 87 ] or label-free using functional groups [ 88 , 89 , 90 ] in electrochemical detection methods.…”

Section: Recent Research On Aβ Detection By Electrochemical Sensorsmentioning

confidence: 99%

SAM-Support-Based Electrochemical Sensor for Aβ Biomarker Detection of Alzheimer’s Disease

Kim

Cho

2023

Biosensors

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Alzheimer’s disease has taken the spotlight as a neurodegenerative disease which has caused crucial issues to both society and the economy. Specifically, aging populations in developed countries face an increasingly serious problem due to the increasing budget for patient care and an inadequate labor force, and therefore a solution is urgently needed. Recently, diverse techniques for the detection of Alzheimer’s biomarkers have been researched and developed to support early diagnosis and treatment. Among them, electrochemical biosensors and electrode modification proved their effectiveness in the detection of the Aβ biomarker at appropriately low concentrations for practice and point-of-care application. This review discusses the production and detection ability of amyloid beta, an Alzheimer’s biomarker, by electrochemical biosensors with SAM support for antibody conjugation. In addition, future perspectives on SAM for the improvement of electrochemical biosensors are also proposed and discussed.

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Electrochemical Immunosensor for the Sensitive Detection of Alzheimer's Biomarker Amyloid‐β (1–42) Using the Heme‐amyloid‐β (1–42) Complex as the Signal Source

Cited by 9 publications

References 78 publications

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

Electrochemical Immunosensors Developed for Amyloid-Beta and Tau Proteins, Leading Biomarkers of Alzheimer’s Disease

SAM-Support-Based Electrochemical Sensor for Aβ Biomarker Detection of Alzheimer’s Disease

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