Electrochemical immunosensor for detection of avian Salmonellosis based on electroactive reduced graphene oxide (rGO) modified electrode

Mahari, Subhasis; Gandhi, Sonu

doi:10.1016/j.bioelechem.2021.108036

Cited by 40 publications

(14 citation statements)

References 27 publications

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“…Advancement in serological assays and biosensors has made them viable alternatives to conventional techniques. Detection methods such as enzyme-linked immunosorbent assays (ELISA), − electrochemical sensors, − field-effect transistors (FET), nanosensors, , and so forth are gaining a lot of attention due to their advantages over traditional methods. Recently, an FTO-based immunosensor was developed for the detection of the receptor-binding domain of SARS-CoV-2 with a limit of detection (LOD) of 0.73 fM .…”

Section: Introductionmentioning

confidence: 99%

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Shahdeo

Chauhan

Majumdar

et al. 2022

ACS Appl. Bio Mater.

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Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet–visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag–Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 μM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.

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Section: Introductionmentioning

confidence: 99%

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Shahdeo

Chauhan

Majumdar

et al. 2022

ACS Appl. Bio Mater.

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“…Memristive devices used in biosensing are usually functionalized with antibodies to detect antigens through a change in the voltage gap in the devices' response due to antibody-antigen interaction. Four main techniques exist in the literature for the attachment of antibodies on the surface of a device, namely direct adsorption [20], [45], [46], affinity approach [47], covalent attachment using Glycidoxypropyltrimethoxysilane (GPTS) [18,48,49], covalent attachment using 1-(3-(Dimethylamino)propyl)-3ethyl carbodiimide hydrochloride (EDC) and Nhydroxysulfosuccinimide (NHS) [16,[50][51][52][53][54][55], and noncovalent attachment [17]. The direct adsorption technique usually starts by treating the device with oxygen plasma for 15 minutes to increase hydroxyl groups (OH-).…”

Section: B Bio-functionalization Of Antibodiesmentioning

confidence: 99%

Memristive Biosensors for Cancer Biomarkers Detection: A Review

et al. 2023

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Detecting cancer biomarkers at an early stage at the clinical level has been the interest of numerous researchers over the years due to its impact on recovery. Therefore, attention is towards fabricating reliable, cost-effective, reproducible, and accurate devices for point-of-care screening. This review aims to highlight the emerging field of memristive biosensors and compare it to similar electrochemical devices used for cancer biomarker detection. The limit of detection (LOD) achieved by memristive biosensors was generally in the femtomolar (fM) range in comparison to field effect transistors (FET) and electrochemical immunosensors, which in most instances exhibited a LOD in the picomolar (pM) and nanomolar (nM) range. Most current memristive biosensors are fabricated using silicon nanowires, which calls for exploring different materials and structures that may lower fabrication complexity and increase reproducibility. This article examines the working principle of memristors for biosensing, the biofunctionalization of antibodies, the interaction between antibodies and antigens and its influence on memristors, as well as fabrication processes and applications of memristors for biosensing. This paper will report on memristor-based biomedical sensors focusing on cancer screening. In addition, the outlook of reduced graphene oxide (rGO) as an active material for sensing will be discussed. Memristors are anticipated to enhance the future of sensing due to their great sensitivity and simplicity of fabrication.

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“…Graphene oxide (GO) is regarded as a high performance electrochemical material because of its electrical conductivity, [25] and large specific surface area. GO also functions as a supporting material for other nanoparticles and macromolecules, [26] and thus it is widely used in the construction of electrochemical sensors [20,27] . Nevertheless, pure graphene is unstable and easily accumulates, [28] so it must be modified [29] .…”

Section: Introductionmentioning

confidence: 99%

Graphene‐Oxide‐Loaded Fe₃O₄‐Pd‐Ag Nanoparticles Allow Sensitive Detection of CEA through a Signal Enhancement Strategy**

Chen

Shi

et al. 2023

ChemistrySelect

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Using graphene oxide (GO)-loaded Fe 3 O 4 -Pd-Ag (GO/Fe 3 O 4 -Pd-Ag) nanoparticles on a gold electrode as a platform, an unlabeled electrochemical immunosensor was fabricated in this study to detect the carcinoembryonic antigen (CEA) with high sensitivity. After modification, the stability and strength of the signal are improved by the increased effective area of the electrode and the synergistic effect of the silver nanoparticles with GO, Fe 3 O 4 , and Pd. The signal intensity decreased as the concentration of CEA increased. Using cyclic voltammetry and electrochemical impedance spectroscopy, the immunosensor layer assembly process was validated. Under optimal conditions, the electrochemical immunosensor had a detection range of 1 pg mL À 1 to 80 ng mL À 1 . The linear equation was I (μA) = 304.563-41.867 × logc CEA (ng mL À 1 ), the correlation coefficient was 0.9967, and the detection limit was 0.2 pg mL À 1 . In addition, the modified electrochemical immunosensor exhibited high selectivity, stability, and repeatability. This electrochemical immunosensor shows a good application prospect in the detection of tumor marker CEA.

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Electrochemical immunosensor for detection of avian Salmonellosis based on electroactive reduced graphene oxide (rGO) modified electrode

Cited by 40 publications

References 27 publications

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Memristive Biosensors for Cancer Biomarkers Detection: A Review

Graphene‐Oxide‐Loaded Fe₃O₄‐Pd‐Ag Nanoparticles Allow Sensitive Detection of CEA through a Signal Enhancement Strategy**

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Electrochemical immunosensor for detection of avian Salmonellosis based on electroactive reduced graphene oxide (rGO) modified electrode

Cited by 40 publications

References 27 publications

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen

Memristive Biosensors for Cancer Biomarkers Detection: A Review

Graphene‐Oxide‐Loaded Fe3O4‐Pd‐Ag Nanoparticles Allow Sensitive Detection of CEA through a Signal Enhancement Strategy**

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Graphene‐Oxide‐Loaded Fe₃O₄‐Pd‐Ag Nanoparticles Allow Sensitive Detection of CEA through a Signal Enhancement Strategy**