Electrochemical immunosensor for carcinoembryonic antigen based on nanosilver-coated magnetic beads and gold-graphene nanolabels

Chen, Huafeng; Tang, Dianping; Zhang, Bing; Liu, Bingqian; Cui, Yuling; Chen, Guonan

doi:10.1016/j.talanta.2012.01.025

Cited by 80 publications

(25 citation statements)

References 44 publications

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“…NMBs can also act as modifiers of the electrotransducer surfaces, creating nanostructures with improved electrochemical response. The improvement in response may come either from the enhancement of the electron transfer or the increased efficiency of biomolecule immobilization [25]. …”

Section: Introductionmentioning

confidence: 99%

A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria

Gikunoo

Abera

Woldesenbet

2014

Sensors

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This paper presents a novel method for direct detection of Plasmodium falciparum histidine rich protein-2 (PfHRP-2) antigen using carbon nanofiber (CNF) forests grown on glass microballoons (NMBs). Secondary antibodies specific to PfHRP-2 densely attached to the CNFs exhibit extraordinary ability for the detection of minute concentrations of Plasmodium species. A sandwich immunoassay protocol was employed, where a glass substrate was used to immobilize primary antibodies at designated capture zones. High signal amplification was obtained in both colorimetric and electrical measurements due to the CNFs through specific binding. As a result, it was possible to detect PfHRP-2 levels as low as 0.025 ng/mL concentration in phosphate buffered saline (PBS) using a visual signal within only 1 min of test duration. Lower limits of 0.01 ng/mL was obtained by measuring the electrical resistivity of the capture zone. This method is also highly selective and specific in identifying PfHRP-2 and other Plasmodium species from the same solution. In addition, the stability of the labeling mechanism eliminates the false signals generated by the use of dyes in current malaria rapid diagnostic test kits (MRDTs). Thus, the rapid, sensitive and high signal amplification capabilities of NMBs is a promising tool for early diagnosis of malaria and other infectious diseases.

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Section: Introductionmentioning

confidence: 99%

A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria

Gikunoo

Abera

Woldesenbet

2014

Sensors

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“…used biofunctionalized magnetic graphene nanosheets as probes and multifunctional nanogold hollow microspheres (GHS) as distinguishable signal tags for the detection of CEA and AFP in biological fluids. In combination with graphene, several other high-performance immunosensors for the detection of various biomarkers were designed using enzyme-like NPs, Ge et al, 2014, Feng et al, 2014, Park et al, 2014, Chen et al, 2012b, Cai et al, 2012. Some polymers, such as chitosan and conducting polymers, could be combined with graphene, resulting in the improvement of the sensitivity of analyte detection Yang et al (2011a).…”

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confidence: 99%

Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials

Yang

Luo

Zhu

et al. 2016

Biosensors and Bioelectronics

339

119

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“…The most common types of immunosensors are found in electrochemical [7,[14][15][16][17][18][19][20][21], optical [22,23], and gravimetric [24] sensors. It is well known that electrochemical detection methods have been able to sensitively and quickly detect biomolecule targets with high selectivity [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors

2016

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Abstract:In this study, we have designed and realized three simple electrochemical bioassays for the detection of the human epidermal growth factor receptor 2 (HER2) cancer biomarker using magnetic beads coupling screen-printed arrays. The different approaches were based on a sandwich format in which affibody (Af) or antibody (Ab) molecules were coupled respectively to streptavidin or protein A-modified magnetic beads. The bioreceptor-modified beads were used to capture the HER2 protein from the sample and sandwich assay was performed by adding the labeled secondary affibody or the antibody. An enzyme-amplified detection scheme based on the coupling of secondary biotinylated bioreceptor with streptavidin-alkaline phosphatase enzyme conjugate was then applied. The enzyme catalyzed the hydrolysis of the electro-inactive 1-naphthyl-phosphate to the electro-active 1-naphthol, which was detected by means of differential pulse voltammetry (DPV). Each developed assay has been studied and optimized. Furthermore, a thorough comparison of the analytical performances of developed assays was performed. Finally, preliminary experiments using serum samples spiked with HER2 protein were also carried out.

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Electrochemical immunosensor for carcinoembryonic antigen based on nanosilver-coated magnetic beads and gold-graphene nanolabels

Cited by 80 publications

References 44 publications

A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria

A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria

Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials

Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors

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