Electrochemical immunosensor for botulinum neurotoxin type-E using covalently ordered graphene nanosheets modified electrodes and gold nanoparticles-enzyme conjugate

Narayanan, J. Shankara; Sharma, Mukesh; Ponmariappan, S.; Sarita, .; Shaik, Mahabul; Upadhyay, S. H.

doi:10.1016/j.bios.2015.02.039

Cited by 74 publications

(39 citation statements)

References 58 publications

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“…Foods are easily contaminated by Clostridium botulinum during processing. Various (impedimetric, voltammetric and amperometric) electrochemical biosensors have been fabricated for BoNT detection [73][74][75][76]. In particular, electrochemical biosensors can achieve detection of this toxin in a fast and meticulous way, and they also provide a robust and cost-effective approach for real-time monitoring of BoNTs.…”

Section: Botulinum Neurotoxinsmentioning

confidence: 99%

“…Recently, 2D nanomaterial-based electrochemical biosensors have been applied to sensitively detect BoNTs in various samples including foods. For instance, Narayanan et al constructed an electrochemical immunosensor of the BoNT serotype E (BoNT/E) by using graphene nanosheets-aryldiazonium salts as transducers [74]. The as-proposed immunosensor shows a low limit of detection (LOD, 5 pg mL −1 ) and can be employed for rapid detection of BoNT/E with a total analysis time of 65 min.…”

Section: Botulinum Neurotoxinsmentioning

confidence: 99%

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Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Jian

et al. 2019

Toxins

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Toxin detection is an important issue in numerous fields, such as agriculture/food safety, environmental monitoring, and homeland security. During the past two decades, nanotechnology has been extensively used to develop various biosensors for achieving fast, sensitive, selective and on-site analysis of toxins. In particular, the two dimensional layered (2D) nanomaterials (such as graphene and transition metal dichalcogenides (TMDs)) and their nanocomposites have been employed as label and/or biosensing transducers to construct electrochemical biosensors for cost-effective detection of toxins with high sensitivity and specificity. This is because the 2D nanomaterials have good electrical conductivity and a large surface area with plenty of active groups for conjugating 2D nanomaterials with the antibodies and/or aptamers of the targeted toxins. Herein, we summarize recent developments in the application of 2D nanomaterial-based electrochemical biosensors for detecting toxins with a particular focus on microbial toxins including bacterial toxins, fungal toxins and algal toxins. The integration of 2D nanomaterials with some existing antibody/aptamer technologies into electrochemical biosensors has led to an unprecedented impact on improving the assaying performance of microbial toxins, and has shown great promise in public health and environmental protection.

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Section: Botulinum Neurotoxinsmentioning

confidence: 99%

Section: Botulinum Neurotoxinsmentioning

confidence: 99%

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Jian

et al. 2019

Toxins

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“…Narayanan et al [115] strongly modified graphene on the surface of a glassy carbon electrode by a series of covalent reactions, and an antibody was further modified on the graphene using the EDC/NHS cross-linking reagent. Using RaMIgG-ALP/AuNPs as a signal amplification marker, the detection of botulinum neurotoxin E was achieved through sandwich immunization with the detection range and detection limit achieving to 10 pg·mL −1 –10 ng·mL −1 and 5.0 pg·mL −1 , respectively.…”

Section: Electrochemical Immunosensors Based On Various Nanomaterialsmentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

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“…This method is very sensitive and specific, but this assay require a huge number of animals. Hence, it is necessary to replace the mouse bioassay with similarly sensitive another assays [16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

ELISA Based Detection of Botulinum Neurotoxin Type F in Red Meat and Canned Fish

2019

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Botulinum neurotoxin (BoNT) is the most toxic protein molecule so far known to animals and humans, due to its extreme toxicity, CDC has been listed as category ‘A' Biological warfare agents. In India, there is no commercial detection system available for the detection of botulism. The present study was aimed to develop an ELISA based detection system for botulinum neurotoxin serotype F in red meat and canned fish. Polyclonal antibodies were generated against the recombinant BoNT/F LC protein and IgG was purified. Sandwich ELISA system was successfully optimized with LOD of ~ 7.8 ng/ml in PBS. Similarly, different concentrations of BoNT/F were spiked in red meat and canned fish and extracted the toxin and the detection LOD was achieved for red meat ~ 62 ng/ml and for canned fish ~ 31 ng/ml. The developed detection system is highly specific. The developed assay will be useful for the screening of botulinum toxins in a large number of food samples.

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Electrochemical immunosensor for botulinum neurotoxin type-E using covalently ordered graphene nanosheets modified electrodes and gold nanoparticles-enzyme conjugate

Cited by 74 publications

References 58 publications

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Nanomaterials for Electrochemical Immunosensing

ELISA Based Detection of Botulinum Neurotoxin Type F in Red Meat and Canned Fish

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