Electrochemical immunoassay for the prostate specific antigen using a reduced graphene oxide functionalized with a high molecular-weight silk peptide

Wang, Yanying; Qu, Ying; Liu, Guishen; Hou, Xiaodong; Huang, Yina; Wu, Wangze; Wu, Kangbing; Li, Chunya

doi:10.1007/s00604-015-1552-2

Cited by 36 publications

(12 citation statements)

References 29 publications

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“…Apart from CVD grown graphene, reduced analogues of GO, such as CRGO, have also been widely used for immunosensing. Wang et al 237 fabricated an impedimetric immunosensor for the detection of prostate specific antigen (PSA). In their work, CRGO was employed as the transducing material and it was functionalized with silk peptide and glutaraldehyde to facilitate the covalent immobilization of anti-PSA via the crosslinking technique.…”

Section: Disease Biomarker Analysismentioning

confidence: 99%

Graphene and its electrochemistry – an update

et al. 2016

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The electrochemistry of graphene and its derivatives has been extensively researched in recent years. In the aspect of graphene preparation methods, the efficiencies of the top-down electrochemical exfoliation of graphite, the electrochemical reduction of graphene oxide and the electrochemical delamination of CVD grown graphene, are currently on par with conventional procedures. Electrochemical analysis of graphene oxide has revealed an unexpected inherent redox activity with, in some cases, an astonishing chemical reversibility. Furthermore, graphene modified with p-block elements has shown impressive electrocatalytic performances in processes which have been historically dominated by metal-based catalysts. Further progress has also been achieved in the practical usage of graphene in sensing and biosensing applications. This review is an update of our previous article in Chem. Soc. Rev. 2010, 39, 4146-4157, with special focus on the developments over the past two years.

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Section: Disease Biomarker Analysismentioning

confidence: 99%

Graphene and its electrochemistry – an update

et al. 2016

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“…Table displays the electroanalytical characteristics of recent reported electrochemical immunosensor for PSA detection and those of the developed immunosensor. As it can be seen, the proposed immunosensor improves the width of the concentration range and the LOD in comparison with some of the recent reported sensors . Although other immunosensors show lower LOD that the achieved in this work (Table ), it has to be considered that the construction of these biosensors involves more complex schemes and higher costs (since more materials, reagents and/or steps are needed).…”

Section: Resultsmentioning

confidence: 99%

“…It retained 86 % of its initial signal after 12 cycles of utilizations (measurement always followed by regeneration), demonstrating the success of the process. This feature is clearly an advantage when compared to the previously reported biosensors for PSA detection that are usually designed to be used only one time .…”

Section: Resultsmentioning

confidence: 99%

Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

et al. 2018

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This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.

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“…Basically, the modified sensing surface is treated with glutaraldehyde (or other aldehydes) that is allowed to react with the antibodies’ functional groups. Like this, the binding between the surface and the biological compound is accomplished through the crosslinking agent , .…”

Section: Antibody Immobilizationmentioning

confidence: 99%

“…The amplitude of this signal decreases with increasing antigen concentration because of the electron transfer hindrance caused by the formed immunocomplex. The most commonly employed redox probes in the label‐free approach for cancer immunosensors are: [Fe(CN) 6 ] 3−/4− , , , Prussian blue (PB) , Ferrocene (Fc) , Ferrocenecarboxylic acid (Fc‐COOH) , Methylene Blue (MB) , Cobalt hexacyanoferrate NPs , thionine (TH) , [Ru(NH 3 ) 6 ] 3+ , H 2 O 2 , Azure I . As an example, the [Fe(CN) 6 ] 3−/4− redox probe was used by Deng et al.…”

Section: Assay Formats (Immunochemical Interactions)mentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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Electrochemical immunoassay for the prostate specific antigen using a reduced graphene oxide functionalized with a high molecular-weight silk peptide

Cited by 36 publications

References 29 publications

Graphene and its electrochemistry – an update

Graphene and its electrochemistry – an update

Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

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