Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Jamal, Rimsha Binte; Shipovskov, Stepan; Ferapontova, Elena E.

doi:10.3390/s20195561

Cited by 14 publications

(5 citation statements)

References 169 publications

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“…For the detection of pathogenic bacteria, aptamers specific to different genus have already been isolated: Vibrio, Staphylococcus, Streptococcus, Haemophilus, or Escherichia [51]. Detection methods classically used with antibodies are also practicable with aptamers [52] such as fluorescence, optical, or electrochemical [53] methods.…”

Section: The Most Promising Ligands Aptamer and Dnazymementioning

confidence: 99%

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

et al. 2022

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Rapid detection of microorganisms is a major challenge in the medical and industrial sectors. In a pharmaceutical laboratory, contamination of medical products may lead to severe health risks for patients, such as sepsis. In the specific case of advanced therapy medicinal products, contamination must be detected as early as possible to avoid late production stop and unnecessary costs. Unfortunately, the conventional methods used to detect microorganisms are based on time-consuming and labor-intensive approaches. Therefore, it is important to find new tools to detect microorganisms in a shorter time frame. This review sums up the current methods and represents the evolution in techniques for microorganism detection. First, there is a focus on promising ligands, such as aptamers and antimicrobial peptides, cheaper to produce and with a broader spectrum of detection. Then, we describe methods achieving low limits of detection, thanks to Raman spectroscopy or precise handling of samples through microfluids devices. The last part is dedicated to techniques in real-time, such as surface plasmon resonance, preventing the risk of contamination. Detection of pathogens in complex biological fluids remains a scientific challenge, and this review points toward important areas for future research.

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Section: The Most Promising Ligands Aptamer and Dnazymementioning

confidence: 99%

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

et al. 2022

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“…However, despite a large number of publications on the aptamer-based sensors, the majority of them are focused on just a few aptamer applications (almost 60% of all publications deal with only eight aptamers) [22,55], while the universal strategies for the aptamer applications in electrochemical bioassays are scarce. In the case of antibodies, the universal protocols have been much better established nowadays [56]. With each new aptamer-protein ligand couple, there is a need of a systematic research of their binding behaviour, conditions and structure of complexes formed, and, not the least, their interfacial behaviour [22].…”

Section: Electrochemical Aptasensorsmentioning

confidence: 99%

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Malecka

Mikuła

Ferapontova

2021

Sensors

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Improved outcomes for many types of cancer achieved during recent years is due, among other factors, to the earlier detection of tumours and the greater availability of screening tests. With this, non-invasive, fast and accurate diagnostic devices for cancer diagnosis strongly improve the quality of healthcare by delivering screening results in the most cost-effective and safe way. Biosensors for cancer diagnostics exploiting aptamers offer several important advantages over traditional antibodies-based assays, such as the in-vitro aptamer production, their inexpensive and easy chemical synthesis and modification, and excellent thermal stability. On the other hand, electrochemical biosensing approaches allow sensitive, accurate and inexpensive way of sensing, due to the rapid detection with lower costs, smaller equipment size and lower power requirements. This review presents an up-to-date assessment of the recent design strategies and analytical performance of the electrochemical aptamer-based biosensors for cancer diagnosis and their future perspectives in cancer diagnostics.

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“…Growing plant diseases caused by plant pathogens promote the development of plant disease monitoring tools. Compared to those traditional bacterial detection technologies, such as polymerase chain reaction, , enzyme-linked immunosorbent assay, , immunofluorescence, , gas chromatography–mass spectrometry, , surface plasmon resonance, and electrochemical methods, the small-molecule fluorescent probes show some advantages including low cost, easy operation, and noninvasive visualization. − Therefore, more and more small-molecule fluorescent probes for bacterial imaging have been reported . However, most of them aim at human pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Mycobacterium tuberculosis, and Streptococcus pneumoniae and were applied in human cell and mouse models .…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Bacterial Plant Diseases via a Nitroreductase-Activated Fluorescent Sensor

Guo

Wang

et al. 2022

Anal. Chem.

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Plant diseases caused by bacteria have become one of the serious problems that threaten human food security, which led to the remarkable reduction of agricultural yields and economic loss. Nitroreductase (NTR), as an important biomarker, is highly expressed in bacteria, and the level of NTR is closely related to the progression of pathogen infection. Therefore, the design of smallmolecule fluorescent sensors targeting NTR is of great significance for the detection and diagnosis of plant pathogenic bacteria. In this study, a new fluorescent sensor targeting NTR was discovered and then successfully applied to the imaging of zebraf ish and pathogenic bacteria. Most importantly, the developed sensor achieved the real-time diagnosis of Brassica napus L. infected with bacteria, which provides a promising tool for examining the temporal and spatial infection of plant pathogens in precision agriculture.

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Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Cited by 14 publications

References 169 publications

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Diagnosis of Bacterial Plant Diseases via a Nitroreductase-Activated Fluorescent Sensor

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