Electrochemical immobilization of enzymes on conducting organic salt electrodes: Preparation of an oxygen independent and interference-free glucose biosensor

Palmisano, Francesco; Centonze, Diego; Malitesta, C.; Zambonin, P. G.

doi:10.1016/0022-0728(94)03743-m

Cited by 13 publications

(9 citation statements)

References 19 publications

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“…Thin polymer films obtained by electropolymerization of o-phenylenediamine (oPD) on platinum at different pH (1,3,5,7) were characterised by XPS.…”

Section: Discussionmentioning

confidence: 99%

“…6 h) a potentiostatic (potential~z0.7 V vs. Ag/AgCl/sat. KCl) electrolysis of the 5 mM oPD solutions at different pH (1,3,5,7).…”

Section: Sample Preparationmentioning

confidence: 99%

“…In particular, poly(o-phenylenediamine), commonly abbreviated as PPD, has been extensively used, both in our laboratory [2][3][4][5][6][7][8] and by other authors (see, for example, refs. 9-18), for the development of biosensors.…”

Section: Introductionmentioning

confidence: 99%

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Spectroscopic investigation on polymer films obtained by oxidation of o-phenylenediamine on platinum electrodes at different pHs

et al. 2001

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An investigation on the structure of poly(o-phenylenediamine) (PPD) films, obtained by electropolymerization of oPD (1,2-diaminobenzene) on platinum at different pH values, was performed by X-ray Photoelectron Spectroscopy (XPS).XPS could be used as a ''bulk'' technique for PPD films analysis, due to their extremely low thickness. The presence of different functionalities, like primary/secondary aminic, iminic and, as minor species, oxygenated groups (carbonyl, oximes) was suggested by curve fitting of carbon (C1s) and nitrogen (N1s) XP spectra. The use of chemical derivatization reactions (CD-XPS) confirmed the presence of primary aminic and hydroxy groups, showing that NH 2 groups are present in the PPD structure even at low pH values, though their amount increases on increasing the pH of polymerization.Optical spectroscopy in the visible region was also performed on the electrolytic solutions at the end of polymerization, suggesting a higher conjugation of the oPD oligomers at low pH, which indirectly confirms XPS findings on the presence of NH 2 groups in the polymer.

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“…Thin polymer films obtained by electropolymerization of o-phenylenediamine (oPD) on platinum at different pH (1,3,5,7) were characterised by XPS.…”

Section: Discussionmentioning

confidence: 99%

“…6 h) a potentiostatic (potential~z0.7 V vs. Ag/AgCl/sat. KCl) electrolysis of the 5 mM oPD solutions at different pH (1,3,5,7).…”

Section: Sample Preparationmentioning

confidence: 99%

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Spectroscopic investigation on polymer films obtained by oxidation of o-phenylenediamine on platinum electrodes at different pHs

et al. 2001

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“…170,264 Besides the Pt and Au electrodes, organic conducting salt (OCS) and carbon including graphite powder, graphite rod, carbon fiber, and carbon microband are often selected as electrode substrate for biosensors. The OCS could be either N-methylphenazinium tetracyanoquinodimethanide prepared by metathesis of phenazine methosulfate and 7,7,8,8-tetracyanoquinodimethane 362,363 or tetrathiafulvalene-p-tetracyanoquino-dimethane. 351 The OCS was tightly pressed onto the paste electrode or used to fill the hole in the working electrode block.…”

Section: Technique Of Enzyme Immobilization Involving Aromatic Diamin...mentioning

confidence: 99%

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

et al. 2002

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“…14,15 The electropolymerization of oPD on CP, which results in improved performance in, for example, the electrocatalysis and accelerated oxidation of hydrogen peroxide with a glucose sensor 16 or the electrocatalytic detection of nicotinamide coenzyme by oPPDmodified CP electrodes, 17 has scarcely been explored. Immobilization of GOx in an oPPD film electrogenerated over an N-methyl phenazinium (NMP)-TCNQ OCS electrode has also been reported previously, 18,19 but no reference has been found concerning bilayer formation on CP or OCS electrodes.…”

Section: Resultsmentioning

confidence: 82%

Three approaches to the development of selective bilayer amperometric biosensors for glucose by in situ electropolymerization

Vidal¹,

García²,

Méndez³

et al. 1999

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Three different glucose oxidase biosensors for the enzymatic determination of glucose, based on bilayer polymer coatings consisting of polypyrrole (PPy) and poly(o-phenylenediamine), were developed. The electrode substrates are Pt metal, carbon paste and an organic conducting salt (tetrathiafulvalene-tetracyanoquinodimethane), which introduces appreciable variability in the electrode construction process. The three sensors were compared with one another and with bare and PPy monolayer-coated sensors in terms of performance in the determination of glucose in a synthetic serum sample. These configurations provide improved selectivity against the interferences of electroactive species such as ascorbic acid and uric acid, frequently present in biological samples, and the differences between them can be taken advantage of in application to different kinds of samples.

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Electrochemical immobilization of enzymes on conducting organic salt electrodes: Preparation of an oxygen independent and interference-free glucose biosensor

Cited by 13 publications

References 19 publications

Spectroscopic investigation on polymer films obtained by oxidation of o-phenylenediamine on platinum electrodes at different pHs

Spectroscopic investigation on polymer films obtained by oxidation of o-phenylenediamine on platinum electrodes at different pHs

Novel Multifunctional Polymers from Aromatic Diamines by Oxidative Polymerizations

Three approaches to the development of selective bilayer amperometric biosensors for glucose by in situ electropolymerization

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