Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

Abe, Hiroya; Kanno, Yusuke; Ino, Kosuke; Inoue, Kumi Y.; Suda, Akira; Kunikata, Ryota; Matsudaira, Masahki; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.5796/electrochemistry.84.364

Cited by 13 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When cell density is too high, an electrode may record events from multiple cells if cells are clustered in its vicinity. Recently, SU-8 microwell structures have been used to trap single cells on passive electrochemical sensor devices [1, 30]. Here we present the implementation of modified surface chemistry and developed a simple and time-saving cell trapping technique on active CMOS devices.…”

Section: Introductionmentioning

confidence: 99%

Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements

Huang

Delacruz

Ruelas³

et al. 2017

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Amperometry is a powerful method to record quantal release events from chromaffin cells and is widely used to assess how specific drugs modify quantal size, kinetics of release, and early fusion pore properties. Surface-modified CMOS-based electrochemical sensor arrays allow simultaneous recordings from multiple cells. A reliable, low-cost technique is presented here for efficient targeting of single cells specifically to the electrode sites. An SU-8 microwell structure is patterned on the chip surface to provide insulation for the circuitry as well as cell trapping at the electrode sites. A shifted electrode design is also incorporated to increase the flexibility of the dimension and shape of the microwells. The sensitivity of the electrodes is validated by a dopamine injection experiment. Microwells with dimensions slightly larger than the cells to be trapped ensure excellent single-cell targeting efficiency, increasing the reliability and efficiency for on-chip single-cell amperometry measurements. The surface-modified device was validated with parallel recordings of live chromaffin cells trapped in the microwells. Rapid amperometric spikes with no diffusional broadening were observed, indicating that the trapped and recorded cells were in very close contact with the electrodes. The live cell recording confirms in a single experiment that spike parameters vary significantly from cell to cell but the large number of cells recorded simultaneously provides the statistical significance.

show abstract

Section: Introductionmentioning

confidence: 99%

Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements

Huang

Delacruz

Ruelas³

et al. 2017

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…In particular, large-scale integration (LSI) technology will be used for incorporating many addressable electrodes into microfluidic devices. Indeed, LSI devices have already been used for electrochemical imaging (Inoue et al, 2012, 2015; Sen et al, 2013a, 2014; Abe et al, 2015, 2016; Kanno et al, 2015a,b, 2016, 2017; Ino et al, 2017, 2018b,c,d, 2019a,b). Since signal amplifiers and switching elements are incorporated into the sensing area, highly sensitive sensors can be incorporated with high density.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Microfluidic devices are widely used for cell analysis, including applications for single-cell analysis, healthcare, environmental monitoring, and organs-on-a-chip that mimic organs in microfluidics. Moreover, to enable high-throughput cell analysis, real-time monitoring, and non-invasive cell assays, electric and electrochemical systems have been incorporated into microfluidic devices. In this mini-review, we summarize recent advances in these systems, with applications from single cells to three-dimensional cultured cells and organs-on-a-chip. First, we summarize microfluidic devices combined with dielectrophoresis, electrophoresis, and electrowetting-on-a-dielectric for cell manipulation. Next, we review electric and electrochemical assays of cells to determine chemical section activity, and oxygen and glucose consumption activity, among other applications. In addition, we discuss recent devices designed for the electric and electrochemical collection of cell components from cells. Finally, we highlight the future directions of research in this field and their application prospects.

show abstract

“…The theoretically calculated current (37.0 pA) was considerably larger than the experimentally observed one. The Nafion coating decreased the electrochemical responses of DA by approximately 60 %, due to reduction in diffusion of DA . The concentration range of both DA and Glu in the actual brain upon stimulations, such as electric pulses and KCl injection are ∼10 μM and ∼40 μM , respectively, indicating that the enzyme‐modified Bio‐LSI devices have sufficient sensitivity for imaging both neurotransmitters in brain samples, such as brain slices.…”

Section: Figurementioning

confidence: 99%

Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device

et al. 2018

Self Cite

View full text Add to dashboard Cite

Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme‐modified large‐scale integration (LSI)‐based amperometric devices, referred to “Bio‐LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer‐modified electrodes. The Os‐HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme‐modified Bio‐LSI.

show abstract

Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

Cited by 13 publications

References 21 publications

Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements

Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements

Electric and Electrochemical Microfluidic Devices for Cell Analysis

Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device

Contact Info

Product

Resources

About