Electrochemical hydrogen sulfide biosensors

Xu, Tailin; Scafa, Nikki; Xu, Li-Ping; Zhou, Shu‐Feng; Al-Ghanem, Khalid Abdullah; Mahboob, Shahid; Fugetsu, Bunshi; Zhang, Xueji

doi:10.1039/c5an02208h

Cited by 150 publications

(97 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the basis of the rapid expansion of new methods for H 2 S detection and quantification, as well as revisions and refinements of current methods, we refer the interested reader to a number of recent reviews on these topics. 15, 43 …”

Section: H2s Detection and Quantificationmentioning

confidence: 99%

“…In the solution phase of the electrode, a strongly basic environment allows the dianionic sulfide reduces Fe(CN) 6 3− to Fe(CN) 6 4− , which is subsequently re-oxidized at the platinum electrode to produce a current relative to H 2 S concentration. One of the advantages of sulfide electrodes is that they can be used directly in mammalian tissues; 43 however, these electrodes cannot provide sub-cellular resolution of sulfide generation, storage, or transport. Even so, sulfide electrodes provide a convenient, time-resolved method for sulfide quantification or detection.…”

Section: H2s Detection and Quantificationmentioning

confidence: 99%

“…Even so, sulfide electrodes provide a convenient, time-resolved method for sulfide quantification or detection. 43 …”

Section: H2s Detection and Quantificationmentioning

confidence: 99%

See 2 more Smart Citations

A practical guide to working with H₂S at the interface of chemistry and biology

2016

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) is the most recently accepted endogenously produced gasotransmitter and is now implicated in a variety of physiological functions. In this tutorial review, our goal is to provide researchers new to the field of H2S chemical biology with practical considerations, pitfalls, and best practices to enable smooth entry into investigations focused on biological H2S. We present practical handling and safety considerations for working with this reactive biomolecule, and cover basic roles of H2S biogenesis and action. Experimental methods for modulating H2S levels, including enzymatic knockout, siRNA silencing, enzymatic inhibition, and use of small molecule H2S donors are highlighted. Complementing H2S modulation techniques, we also highlight current strategies for H2S detection and quantification.

show abstract

Section: H2s Detection and Quantificationmentioning

confidence: 99%

Section: H2s Detection and Quantificationmentioning

confidence: 99%

See 1 more Smart Citation

A practical guide to working with H₂S at the interface of chemistry and biology

2016

View full text Add to dashboard Cite

show abstract

“… in an H 2 S detection system. Detection of the sulfide species by using biosensors has been focused more on enzyme‐based biosensors . Therefore, using other bioelements such as proteins can be considered promising.…”

Section: Introductionmentioning

confidence: 99%

Novel microfluidic graphene oxide–protein amperometric biosensor for detecting sulfur compounds

Ghaemi

Abdi

Javadi

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems in human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. In this study, a novel portable amperometric graphene oxide-protein biosensor platform is investigated. The main characteristic of this structure is the implementation of a microfluidic configuration. With albumin metalloprotein as the biorecognition element, graphene oxide was synthesized and characterized by transmission electron microscopy and Fourier-transform infrared spectroscopy (FTIR). Albumin protein was stabilized on the surface of graphene oxide by the application of the N-(3-dimethylamionpropyl)-N-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide method. The stabilization was confirmed by FTIR and electrochemistry analyses. The calibration curve of sulfur concentration was determined. When the graphene oxide-protein complex was stabilized by nephion on the surface of the microfluidic system, the response time reduced to 50 Sec, which is a relatively faster response among the similar studies and validated the significant effect of the microfluidic system. The nanosystem had an optimized pH of 7.4 and exhibited high sensitivity in determining sulfide. The results confirm that the portable graphene oxide-protein nanosystem has a fast and accurate response in detecting sulfide.

show abstract

“…As a result, several types of superwettable microchips have been explored depending on different detection signals such as surface-enhanced Raman scattering (SERS), [26][27][28] colorimetric, [29][30][31][32][33] and fluorescence enhancement effect, [34][35][36] to perform demanding sensing tasks. [37][38][39] Electrochemical biosensors, as one of the most common methods of analytical research, have been widely used to sensitively detect biomolecules, [40][41][42][43] such as glucose, 44 nitric oxide, 45 hydrogen peroxide, 46 hydrogen sulfide, 47 DNA /miRNA, [48][49][50] and proteins. 51 However, electrochemical biosensing are usually carried out in a large solution system.…”

mentioning

confidence: 99%

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

Song

Gao

et al. 2018

ACS Sens.

Self Cite

View full text Add to dashboard Cite

Bioinspired superwettable micropatterns that combine two extreme states of superhydrophobicity and superhydrophilicity with the ability to enrich and absorb microdroplets are suitable for versatile and robust sensing applications. Here we introduce a superwettable microchip that integrates superhydrophobic–superhydrophilic micropatterns and a nanodendritic electrochemical biosensor toward the detection of prostate cancer biomarkers. On the superwettable microchip, the superhydrophobic area could confine the microdroplets in superhydrophilic microwells; such behavior is extremely helpful for reducing the amount of analytical solution. In contrast, superhydrophilic microwells exhibit a high adhesive force toward microdroplets, and the nanodendritic structures can improve probe-binding capacity and response signals, thus greatly enhancing the sensitivity. Sensitive and selective detection of prostate cancer biomarkers including miRNA-375, miRNA-141, and prostate-specific antigen on a single microchip is also achieved. Such a superwettable microchip with high sensitivity, low sample volume, and upside-down detection capability in a single microdroplet shows great potential to fabricate portable devices toward complex biosensing applications.

show abstract

Electrochemical hydrogen sulfide biosensors

Cited by 150 publications

References 98 publications

A practical guide to working with H₂S at the interface of chemistry and biology

A practical guide to working with H₂S at the interface of chemistry and biology

Novel microfluidic graphene oxide–protein amperometric biosensor for detecting sulfur compounds

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

Contact Info

Product

Resources

About

Electrochemical hydrogen sulfide biosensors

Cited by 150 publications

References 98 publications

A practical guide to working with H2S at the interface of chemistry and biology

A practical guide to working with H2S at the interface of chemistry and biology

Novel microfluidic graphene oxide–protein amperometric biosensor for detecting sulfur compounds

Superwettable Electrochemical Biosensor toward Detection of Cancer Biomarkers

Contact Info

Product

Resources

About

A practical guide to working with H₂S at the interface of chemistry and biology

A practical guide to working with H₂S at the interface of chemistry and biology