Electrochemical genosensor design: immobilisation of oligonucleotides onto transducer surfaces and detection methods

Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

“…Recent progresses in the development of electrochemical DNA-hybridisation biosensors have been summarised in some excellent reviews (Pividori et al, 2000;Yang et al, 1997;Wang, 2000;Palecek and Fojta, 2001;Palecek, 2002). Hence, this review will be focused on electrochemical DNA biosensors and sensors developed using carbon and gold electrodes as the transducer.…”

Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology

“…27,35,[42][43][44][45][46][47][48][49][50][51] The bioarray used in this study is a new oligonucleotide array, consisting of prequalified oligonucleotides covalently attached via a noncontact dispense method to a threedimensional gel matrix affixed to a glass surface. This paper describes the use of this new oligonucleotide array in a biological validation study involving the treatment of rats with carbon tetrachloride (CCl 4 ), a well-established model of chemically induced hepatotoxicity.…”

DNA-Based Nanotechnology Biosensors for Surgical Diagnosis

“…Materials supporting DNA sequences in biosensors are often called transducers. Currently, there are three general types of immobilization that are widely used: adsorption, avidin-biotin complexation, and covalent attachment (Pividori et al 2000).…”

“…The inherent aqueous stability of the avidin-biotin complex renders the system easy to handle. Because of the presence of a large protein layer, there might be some nonspecific interactions to block DNA-DNA binding sites (Pividori et al 2000;Marrazza et al 1999). Although usually such blocking would not significantly impact detection, due to the extremely high selectivity of hybridization, a more desired and direct approach has been investigated for DNA immobilization: covalent bond connections.…”