Electrochemical DNA Sensors Based on Nanostructured Polymeric Materials for Determination of Antitumor Drugs

Porfireva, Anna; Hianik, Tibor; Evtugyn, Gennady

doi:10.1007/978-3-030-55490-3_11

Cited by 1 publication

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“…From other sensors utilized in determination of biologically active low molecular compounds, DNA-based assay is considered as one of most promising due to high variety of the species detected, higher stability of DNA oligonucleotides and aptamers against proteins, and simpler detection mode [ 9 , 10 ]. In particular, DNA-based sensors have been described for sensitive determination of antitumor drugs [ 11 , 12 , 13 , 14 ], reactive oxygen species [ 15 ], and antioxidants [ 16 , 17 , 18 ]. Aptamer-based biosensors detect with very high sensitivity mycotoxins, drugs, and cancer biomarkers [ 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical DNA Sensor Based on Poly(Azure A) Obtained from the Buffer Saturated with Chloroform

Porfireva

Plastinina

Evtugyn

et al. 2021

Sensors

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Electropolymerized redox polymers offer broad opportunities in detection of biospecific interactions of DNA. In this work, Azure A was electrochemically polymerized by multiple cycling of the potential in phosphate buffer saturated with chloroform and applied for discrimination of the DNA damage. The influence of organic solvent on electrochemical properties of the coating was quantified and conditions for implementation of DNA in the growing polymer film were assessed using cyclic voltammetry, quartz crystal microbalance, and electrochemical impedance spectroscopy. As shown, both chloroform and DNA affected the morphology of the polymer surface and electropolymerization efficiency. The electrochemical DNA sensor developed made it possible to distinguish native and thermally and chemically damaged DNA by changes in the charge transfer resistance and capacitance.

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