Electrochemical DNA hybridization detection using peptide nucleic acids and [Ru(NH3)6]3+ on gold electrodes

Steichen, Marc; Decrem, Yves; Godfroid, Edmond; Buess‐Herman, C.

doi:10.1016/j.bios.2006.10.041

Cited by 114 publications

(97 citation statements)

References 40 publications

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“…Notably, higher temperatures, solutions with low ionic strength and shorter sequences can be employed. 29,30 Electrochemical DNA detection is facilitated by redox-active probes, which are introduced into the PNA recognition layer as free-diffusing redox mediator, 31 redox-active intercalators or minor-groove binders 28,[32][33][34][35] or as covalently bound redox labels. [36][37][38][39] Label-free PNA-based biosensors were, in general, exploited using electrochemical impedance spectroscopy, 31,40,41 Scanning…”

Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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Peptide Nucleic Acids (PNAs) are non-natural DNA/RNA analogues with favourable physicochemical properties and promising applications. Discovered nearly 20 years ago, PNAs have recently re-gained quite a lot of attention. In this Perspective article, we discuss the latest advances on the preparation and utilisation of PNA monomers and oligomers containing metal complexes. These metalconjugates have found applications in various research fields such as in the sequence-specific detection of nucleic acids, in the hydrolysis of nucleic acids and peptides, as radioactive probes or as modulators of PNA˙DNA hybrid stability, and last but not least as probes for molecular and cell biology.

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Section: Metal-containing Pnas For Biosensing Purposesmentioning

confidence: 99%

Metal-containing peptide nucleic acid conjugates

2011

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“…5A). Hybridization of the target DNA resulted in current increase since the duplex DNA can bind to more [Ru(NH3)6] 3+ complexes [30,31]. The amount of signal increase was comparable between the thiolated and non-thiolated probe DNA.…”

Section: Resultsmentioning

confidence: 98%

Fast assembly of non-thiolated DNA on gold surface at lower pH

Jiang¹,

Materón²,

Sotomayor³

et al. 2013

Journal of Colloid and Interface Science

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In a typical protocol for attaching DNA to a gold electrode, thiolated DNA is incubated with the electrode at neutral pH overnight. Here we report fast adsorption of non-thiolated DNA oligomers on gold electrodes at acidic pH (i.e., pH ~3.0). The peak-to-peak potential difference and the redox peak currents in typical cyclic voltammetry of [Fe(CN)6] 3-are investigated to monitor the attachment. Compared with incubation at neutral pH, the lower pH can significantly promote the adsorption processes, enabling efficient adsorption even in 30 min. The adsorption rate is DNA concentration-dependent, while the ionic strength shows no influence. Moreover, the adsorption is base-discriminative, with a preferred order of A >C>>G, T, which is attributed to the protonation of A and C at low pH and their higher binding affinity to gold surface. The immobilized DNA is functional and can hybridize with its complementary DNA but not a random DNA. This work is promising to provide a useful time-saving strategy for DNA assembly on gold electrodes, allowing fast fabrication of DNA-based biosensors and devices.3

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“…[3][4][5] Of all DNA detection methods, electrochemical methods have the advantages of low cost, simplicity, rapid response, and impressive compatibility with miniaturization. [6][7][8] In electrochemical methods, hybridization is detected by a signal change that is mostly generated from redox labels such as [Ru(NH 3 ) 6 ] 3+/2+ , 6,7,9,10 [Fe(CN) 6 ] 4-/3-, [11][12][13][14] [Co(phen) 3 ] 3+/2+ , 15 methylene blue, 16 ferrocene, 8,17 and conductive polymers. 2,4 A sandwich-type assay is widely used to detect the target DNA in electrochemical methods, especially in methods that involve the use of semiconductor nanoparticles, 18 enzymes, 19 and vesicles 20 for labeling.…”

Section: Introductionmentioning

confidence: 99%

“…When PNA on gold electrodes is hybridized with the target DNA, the bound target DNA can be detected by redox-active cations such as [Ru (NH3 , which bind with the negatively charged DNA by means of electrostatic attraction. 6,7,9,10,15 A ferrocene dendrimer (Fc-Den) is a redox-active cation that can perform multielectron-transfer. Because a dendrimer partially contains ferrocenes by covalent bonding, an Fc-Den can interact with the electrode via a redox reaction of ferrocenes.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical DNA Detection Based on Electrostatic Interaction between DNA and Ferrocene Dendrimers

Lee¹,

Kim²,

Hwang³

et al. 2010

Bulletin of the Korean Chemical Society

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A label-free DNA detection method was developed for a simple electrochemical DNA sensor with a short assay time. Self-assembled monolayers of peptide nucleic acid were used as a probe on gold electrodes. The formation of the self-assembled monolayers on the gold electrodes was successfully checked by means of cyclic voltammetry. The target DNA, hybridized with peptide nucleic acid, can be detected by the anodic peak current of ferrocene dendrimers, which interact electrostatically with the target DNA. This anodic peak current was measured by square wave voltammetry at 0.3 V to decrease the detection limit on the order of the nanomolar concentrations. As a result, the label-free electrochemical DNA sensor can detect the target DNA in concentrations ranging from 1 nM to 1 μM with a detection limit of 1 nM.

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Electrochemical DNA hybridization detection using peptide nucleic acids and [Ru(NH3)6]3+ on gold electrodes

Cited by 114 publications

References 40 publications

Metal-containing peptide nucleic acid conjugates

Metal-containing peptide nucleic acid conjugates

Fast assembly of non-thiolated DNA on gold surface at lower pH

Label-Free Electrochemical DNA Detection Based on Electrostatic Interaction between DNA and Ferrocene Dendrimers

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