Electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Hájková, Andrea; Barek, Jiřı́; Vyskočil, Vlastimil

doi:10.1016/j.bioelechem.2017.02.003

Cited by 59 publications

(24 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although these techniques are frequently used for identification and determination of NPAHs, less expensive polarographic and voltammetric techniques play a significant role in the nanomolar range detection of these ecologically important compounds [5,[19][20][21]. Moreover, sensitivity of voltammetric methods can be often increased by their combination with preliminary separation and preconcentration steps [22,23].…”

Section: Introductionmentioning

confidence: 99%

Polarographic and voltammetric determination of genotoxic 4-nitroindane at mercury electrodes

2015

Self Cite

View full text Add to dashboard Cite

Determination and electrochemical investigation of genotoxic nitrated polycyclic aromatic hydrocarbon 4-nitroindane (4-NI) were carried out using direct current tast polarography (DCTP) and differential pulse polarography (DPP) at a classical dropping mercury electrode (DME) and using direct current voltammetry (DCV), differential pulse voltammetry (DPV), and cyclic voltammetry (CV) at a hanging mercury drop minielectrode (HMDmE). The following optimal media for the determination of 4-NI were found: methanol-BrittonRobinson (BR) buffer of pH 12.0 (1:1) for DCTP and DPP, methanol-BR buffer of pH 9.0 (1:9) for DCV, and methanol-BR buffer of pH 8.0 (1:9) for DPV. For CV investigation, mixtures of methanol-BR buffer of pH 3.0, 8.0, and 12.0 (1:1) were used. The limits of quantification were: 0.65 lmol dm -3 (DCTP at the DME), 0.10 lmol dm -3 (DPP at the DME), 0.075 lmol dm -3 (DCV at the HMDmE), and 0.070 lmol dm -3 (DPV at the HMDmE). An attempt to increase the determination sensitivity using adsorptive accumulation of 4-NI on the HMDmE surface was not successful. The newly developed polarographic and voltammetric methods were successfully applied for the determination of 4-NI in model samples of drinking and river water. Graphical abstract -300 -400 -500 -600 -700 -800 -10 -15 -20 -25 -30 NO 2 I / nA E / mV 0 . 0 0 . 4 0 . 8 1 . 2 0 -4 -8 -12 c / µmol dm -3

show abstract

Section: Introductionmentioning

confidence: 99%

Polarographic and voltammetric determination of genotoxic 4-nitroindane at mercury electrodes

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reactive oxygen species (ROS) are composed of numerous active substances with oxygen atom composing of single electron, which is able to initiate the oxidation of DNA . Growing evidences strongly suggest that many valid strategies are required to repair damages of DNA and to determine oxidative products from DNA damage caused by ROS .…”

Section: Introductionmentioning

confidence: 99%

Enhancing Antioxidant Effect against Peroxyl Radical‐Induced Oxidation of DNA: Linking with Ferrocene Moiety!

Liu

2019

The Chemical Record

View full text Add to dashboard Cite

As a major member in the family of reactive oxygen species, peroxyl radical is able to abstract hydrogen atom from 4‐position of ribose, leading to the collapse of DNA strand. Thus, inhibiting oxidative stress with exogenous antioxidants acts as a promising strategy to protect the integrity of DNA structure and is thereby suggested to be a pathway against developments of related diseases. Ferrocene as an organometallic scaffold is widely applied in the design of organometallic drugs, and redox of Fe(II)/Fe(III) in ferrocene offers advantage for providing electron to radicals. Presented herein are our ongoing studies on ferrocene‐appended antioxidants, including McMurry reaction applied to construct ferrocifen; Aldol condensation used to prepare ferrocenyl curcumin; Povarov reaction employed to prepare ferrocenyl quinoline; Biginelli reaction used to construct ferrocenyl dihydropyrimidine; Groebke reaction used to synthesize ferrocenyl imidazo[1,2‐a]pyridine; and Passerini three‐component reaction as well as Ugi four‐component reaction applied to synthesize α‐acyloxycarboxamide and bisamide, respectively. It is found that ferrocene moiety is able to enhance antioxidative effect of the aforementioned scaffolds even without the aid of phenolic hydroxyl group. The role of ferrocene in enhancing antioxidative effect can be attributable to trapping radicals, decreasing oxidative potential, and increasing the affinity toward DNA strand. Therefore, ferrocene is worthy to be taken into consideration in the design of drugs in relation to DNA oxidation.

show abstract

“…To date, various electrochemical sensors for DNA damage, DNA interactions with drugs, environmental pollutants, and other potentially genotoxic species have been developed and reviewed by various authors [9,10,11,12,13,14]. However, our group is the only one to develop metabolite-generating microfluidic electrochemiluminescent (ECL) arrays for predicting metabolic genotoxicity chemistry of xenobiotics.…”

Section: Introductionmentioning

confidence: 99%

Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays

Bist

Bano

Rusling

2017

Sensors

View full text Add to dashboard Cite

This review describes progress in the development of electrochemiluminescent (ECL) arrays aimed at sensing DNA damage to identify genotoxic chemistry related to reactive metabolites. Genotoxicity refers to chemical or photochemical processes that damage DNA with toxic consequences. Our arrays feature DNA/enzyme films that form reactive metabolites of test chemicals that can subsequently react with DNA, thus enabling prediction of genotoxic chemical reactions. These high-throughput ECL arrays incorporating representative cohorts of human metabolic enzymes provide a platform for determining chemical toxicity profiles of new drug and environmental chemical candidates. The arrays can be designed to identify enzymes and enzyme cascades that produce the reactive metabolites. We also describe ECL arrays that detect oxidative DNA damage caused by metabolite-mediated reactive oxygen species. These approaches provide valuable high-throughput tools to complement modern toxicity bioassays and provide a more complete toxicity prediction for drug and chemical product development.

show abstract

Electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Cited by 59 publications

References 29 publications

Polarographic and voltammetric determination of genotoxic 4-nitroindane at mercury electrodes

Polarographic and voltammetric determination of genotoxic 4-nitroindane at mercury electrodes

Enhancing Antioxidant Effect against Peroxyl Radical‐Induced Oxidation of DNA: Linking with Ferrocene Moiety!

Screening Genotoxicity Chemistry with Microfluidic Electrochemiluminescent Arrays

Contact Info

Product

Resources

About