Electrochemical DNA biosensor fabrication with hollow gold nanospheres modified electrode and its enhancement in DNA immobilization and hybridization

Li, Shufeng; Liu, Jing; Han, Xiaoping; Cui, Yupeng; Wang, Wei

doi:10.1016/j.bios.2009.11.026

Cited by 92 publications

(26 citation statements)

References 44 publications

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“…The genosensor prepared in a similar way, using ssDNA probes possessing different length of spacer between the SH group and the oligonucleotide, displayed one order of magnitude higher detection limit only for 23-mer complementary sequences [59]. The lower detection limits in comparison to the results presented here were reported for genosensors with incorporated redox active intercalators [53,54,57,58]. However, the sensitivity was estimated using only short oligonucleotide sequences.…”

Section: Application Of Genosensor For Monitoring Of Hybridization Prcontrasting

confidence: 69%

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

et al. 2012

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The working principle of a genosensor is based on the mechanism of ion‐channel mimetic sensors. The analytical signals generated upon hybridization processes were recorded by a redox active marker [Fe(CN)6]3−/4− present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20‐mer complementary oligonucleotide sequence, and also of the PCR products containing the complementary 20‐mer sequence in various positions, with detection limits in the 10 pM range. The noncomplementary 20‐mer oligonucleotide sequence as well as the PCR product without complementary region generated very weak response. The good discrimination of the position of the complementary part in the PCR products was observed.

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Section: Application Of Genosensor For Monitoring Of Hybridization Prcontrasting

confidence: 69%

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

et al. 2012

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“…To enhance the detection of short DNA sequences Liu et al 194 fabricated a DNA biosensor based on hollow gold nanopartices. The hollow gold nanoparticles are fabricated based on a displacement of Co nanoparticle, characterized using CV and electrochemical impedance spectroscopy, have spike-like morphology of their surface which enhances the immobilizations and hybridization of the DNA and after two weeks still maintained about 95% of their initial response with a wide linear range, 1 pM to 10 nM, and low detection limit.…”

Section: Biosensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors

et al. 2011

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By using a solid KCl melt in conjunction with an Ag/AgCl component, Vanau et al. 8 were able to fabricate an SSRE designed specifically for beverage industry applications. They found that this SSRE had a wide range of pH use, stable potentials, and small drift potentials (1 mV at room temperature over 3 months time). The reported stability and lifetime of this reference electrode make it particularly applicable for the food industry. In addition to food industry, engine diagnostics are utilizing SSRE to assess efficiency. Oxygen sensors have long been utilizing yttria-stabilized zirconia-based potentiometric components. Recently, Mn-based oxides have been studied to find a suitable SSRE to ensure accurate on-board diagnosis for engines. Miura et al. 9 found Mn 2 O 3 -sensing electrodes to function with excellent sensitivity and has great potential for miniaturization.Rius-Ruiz et al. 10 recently reported a carbon nanotube (CNT)-based SSRE. The most successful design tested utilized a photopolymerized n-butyl acrylate polymer in conjuction with SWCNTs, acting as the transducer layer. This type of transducer layer was often superior to alternative solid transducers, and the resulting SSRE proved insensitive to room lighting. The superior characteristics of this reference electrode as well as its ease of fabrication allow for potential widespread usage in a multitude of systems.Other Papers of Interest. To ensure reference electrodes keep up with current miniaturization trends, it has become challenging to find suitable materials for all potential applications. One such challenging arena is that of steel corrosion monitoring. Muralidharan et al. 11 developed a NiFe 2 O 4 reference electrode due to its superior fabrication cost and ease and its resistance to corrosion. The resulting electrode exhibited stability and low polarization currents in a calcium hydroxide solution, which is commonly used in concrete environments.Shibata et al. 12 examined the stability of an Ag/AgCl reference electrode with the novel salt bridge 1-methyl-3-octylimidazolium bis(trifluoromethanesulfonyl)-amide. This bridge was proposed and found to be superior in order to avoid the interference found using alternate ionic liquid salt bridges, such as KCl, while experimenting in phthalate buffer. Further evaluation of stability is necessary; however, the initial findings suggest this ionic liquid salt bridge is promising. Huang et al. 13 recently developed a working system using a Nafion strip membrane as an ion-conducting bridge, allowing for electrochemical measurements in pure water. By using this bridge, it is possible to avoid potential leaching contaminants and gain higher accuracy.Measuring the electrodeposition of boron melts is a challenge to researchers due to the unknown chemistry of KF-KCl-KBrF 4 . Pal et al. 14 fabricated an Ag/AgCl reference electrode that was found to be reversible and to have suitable stability and nonpolarizability. This will allow for the first available measurements of KF-KCl-KBrF 4 chemistry at high tempera...

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“…The high specificity of this genosensing system indicates the potential application in DNA hybridization determination for the diagnosis of diseases. For comparation, the linear ranges, probes and target DNA obtained with some methods for the determination of DNA are listed in Table 1 [11,[32][33][34][35].…”

Section: Selectivity Of the Proposed Methodsmentioning

confidence: 99%

A DNA biosensor based on resonance light scattering using unmodified gold bipyramids

Chen

et al. 2012

Microchim Acta

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We report on a novel biosensor for determining sequence-specific DNA. It is based on resonance light scattering (RLS) caused by the aggregation of gold bipyramids. These display localized surface plasmon resonance and can be used as a bioprobe. The absorption spectra and the transmission electron micrographs provide visual evidence of the aggregation of the gold bipyramids in the presence of DNA. The RLS intensity of the gold bipyramids increases with the concentration of the target DNA. The method was successfully applied to the determination of a 30-mer singlestranded oligonucleotide and works over the 0.1-10 nM concentration range.

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Electrochemical DNA biosensor fabrication with hollow gold nanospheres modified electrode and its enhancement in DNA immobilization and hybridization

Cited by 92 publications

References 44 publications

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

Electrochemical Sensors and Biosensors

A DNA biosensor based on resonance light scattering using unmodified gold bipyramids

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