Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink

Molinari, Judith; Flórez, Laura; Medrano, Anahí V.; Monsalve, Leandro N.; Ybarra, Gabriel

doi:10.3390/bios8040109

Cited by 12 publications

(5 citation statements)

References 21 publications

(26 reference statements)

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“…The Ybarra group [ 73 ] proposed an electrochemical biosensor based on a sandwich-type immunoassay for the detection of β-LB, using screen-printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads (PSBs) bearing several carboxylic groups for the bioreceptor immobilization.…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

“…Another factor to consider is the possibility of using portable user-friendly devices that can allow analysis, for example, at the restaurant or at home [13,107]. In this framework, the integrated exogenous antigen testing (iEAT) is a very interesting example of a user-friendly and simple smartphone-based electrochemical food analyzer based on a sandwich immunomagnetic assay format [108] in analogy with the examples of immunosensors reported above [73,74,82,89,97]. The target proteins were gliadin in wheat, Ara h 1 in peanut, Cor a 1 in hazelnut, casein in milk, and OVA in egg white.…”

Section: Immunosensorsmentioning

confidence: 99%

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Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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Food allergy has been indicated as the most frequent adverse reaction to food ingredients over the past few years. Since the only way to avoid the occurrence of allergic phenomena is to eliminate allergenic foods, it is essential to have complete and accurate information on the components of foodstuff. In this framework, it is mandatory and crucial to provide fast, cost-effective, affordable, and reliable analysis methods for the screening of specific allergen content in food products. This review reports the research advancements concerning food allergen detection, involving electrochemical biosensors. It focuses on the sensing strategies evidencing different types of recognition elements such as antibodies, nucleic acids, and cells, among others, the nanomaterial role, the several electrochemical techniques involved and last, but not least, the ad hoc electrodic surface modification approaches. Moreover, a selection of the most recent electrochemical sensors for allergen detection are reported and critically analyzed in terms of the sensors’ analytical performances. Finally, advantages, limitations, and potentialities for practical applications of electrochemical biosensors for allergens are discussed.

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Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Section: Immunosensorsmentioning

confidence: 99%

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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“…The use of particulated carriers such as polystyrene microspheres, onto which biomolecules can be immobilized, has been proposed as an alternative to direct linking to CNTs for the preparation of inks for printed enzyme electrodes [ 32 ]. This approach allows enzyme immobilization without significantly affecting the electrical and electrochemical behavior of CNTs.…”

Section: Introductionmentioning

confidence: 99%

Fully Inkjet-Printed Biosensors Fabricated with a Highly Stable Ink Based on Carbon Nanotubes and Enzyme-Functionalized Nanoparticles

et al. 2021

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Enzyme inks can be inkjet printed to fabricate enzymatic biosensors. However, inks containing enzymes present a low shelf life because enzymes in suspension rapidly lose their catalytic activity. Other major problems of printing these inks are the non-specific adsorption of enzymes onto the chamber walls and stability loss during printing as a result of thermal and/or mechanical stress. It is well known that the catalytic activity can be preserved for significantly longer periods of time and to harsher operational conditions when enzymes are immobilized onto adequate surfaces. Therefore, in this work, horseradish peroxidase was covalently immobilized onto silica nanoparticles. Then, the nanoparticles were mixed into an aqueous ink containing single walled carbon nanotubes. Electrodes printed with this specially formulated ink were characterized, and enzyme electrodes were printed. To test the performance of the enzyme electrodes, a complete amperometric hydrogen peroxide biosensor was fabricated by inkjet printing. The electrochemical response of the printed electrodes was evaluated by cyclic voltammetry in solutions containing redox species, such as hexacyanoferrate (III/II) ions or hydroquinone. The response of the enzyme electrodes was studied for the amperometric determination of hydrogen peroxide. Three months after the ink preparation, the printed enzyme electrodes were found to still exhibit similar sensitivity, demonstrating that catalytic activity is preserved in the proposed ink. Thus, enzyme electrodes can be successfully printed employing highly stable formulation using nanoparticles as carriers.

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“…In addition, acrylic inks containing functionalized MWCNTs have been applied to the fabrication of electroluminescent devices, capacitive sensors, and wearable electronics by screen printing, showing high electrical conductivity, mechanical stability, and even resistance to folding [28]. In electrochemical applications, acrylic MWCNT inks have been deposited on glassy carbon electrodes, resulting in improved electrocatalytic activities [29,30]. Moreover, flexible electrodes made of the acrylic ink have been used as support for enzyme immobilization with remarkable biosensing properties [29].…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotube Film Electrodes with Acrylic Additives: Blocking Electrochemical Charge Transfer Reactions

et al. 2020

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Carbon nanotubes (CNTs) processed into conductive films by liquid phase deposition technologies reveal increasing interest as electrode components in electrochemical device platforms for sensing and energy storage applications. In this work we show that the addition of acrylic latex to water-based CNT inks not only favors the fabrication of stable and robust flexible electrodes on plastic substrates but, moreover, sensitively enables the control of their electrical and electrochemical transport properties. Importantly, within a given concentration range, the acrylic additive in the films, being used as working electrodes, effectively blocks undesired faradaic transfer reactions across the electrode–electrolyte interface while maintaining their capacitance response as probed in a three-electrode electrochemical device configuration. Our results suggest a valuable strategy to enhance the chemical stability of CNT film electrodes and to suppress non-specific parasitic electrochemical reactions of relevance to electroanalytical and energy storage applications.

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Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink

Cited by 12 publications

References 21 publications

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Fully Inkjet-Printed Biosensors Fabricated with a Highly Stable Ink Based on Carbon Nanotubes and Enzyme-Functionalized Nanoparticles

Carbon Nanotube Film Electrodes with Acrylic Additives: Blocking Electrochemical Charge Transfer Reactions

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