Electrochemical determination of urea using a gold nanoparticle-copolymer coated-enzyme modified gold electrode

Korkut, Şeyda; Uzunçar, Sinan; Kilic, Muhammet Samet; Hazer, Baki

doi:10.1080/10739149.2018.1447486

Cited by 21 publications

(8 citation statements)

References 35 publications

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“…The voltammetric behaviour was found to be similar to platforms decorated with metal nanoparticles or graphene nanoplatelets. [59,60] Urea detection was most efficient (for both mono-and bienzyme biosensors) at À 0.2 V vs. Ag/AgCl (Figure 1a and 2a), similar to what has been previously reported, [61][62][63] and in accordance with ammonia electrooxidation at the NFs electrode. Glucose detection was optimized at 0.65 V vs. Ag/AgCl (Figure 2c).…”

Section: Optimization Of Operational Parameterssupporting

confidence: 88%

Discriminative detection of glucose and urea with a composite polymer nanofiber based matrix

Apetrei,

Guven,

Camurlu

2024

ChemistrySelect

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Detection and quantification of glucose and urea are fundamental in medical analysis, real‐time monitoring, and point of care analysis. However, such analyses generally require sophisticated instrumentation and specialized personnel. Furthermore, discriminative detection of multiple analytes is often necessary for treatment and diagnosis. To address this challenge, this study describes a proof‐of‐concept for discriminative sensitive amperometric detection of both analytes. This was achieved by the fabrication of a polymer‐MWCNTs composite nanofiber matrix upon which glucose oxidase (GOD) and urease were co‐immobilized to allow detection of the corresponding substrates (glucose and urea). The (PAN(−MWCNTs)/Urease‐GOD) biosensor showed good performance in: (i) glucose biosensing with sensitivity of 31±5 μAmM−2cm−2 within an extended linear range (0.1–5.0 mM) and a limit of detection of 3.7 μM, and (ii) urea detection at very low concentration (4–30 and 30–90 μM) with sensitivity up to 350±19 μAmM−2cm−2 and very low LOD (0.1 μM). The analogous mono‐enzyme platform for urea detection (PAN(−MWCNTs)/Urease) showed very similar performance as the bi‐enzyme sensor, suggesting little to no influence of co‐immobilization on Urease activity, thus confirming that the two catalytic processes are independent of each other. The biosensors exhibited high accuracy in the detection of both analytes (at normal and pathological levels) in human serum, thus proving that the biosensor described herein can have practical applications in healthcare and remote patient monitoring.

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Section: Optimization Of Operational Parameterssupporting

confidence: 88%

Discriminative detection of glucose and urea with a composite polymer nanofiber based matrix

Apetrei,

Guven,

Camurlu

2024

ChemistrySelect

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“…Detailed experiments were conducted to determine the precise potential of the phosphate measurement specially with DPV technique which uses potential pulse series of increasing amplitude while the potential is also swept with pulse series in our study. This technique allows time for the non faradaic (charging) current to decay, and as a result, sensitivity is increased significantly while monitoring the little quantities of surface‐attached molecules . Figure A shows the DPV experiment set up by sweeping the potential between −1 V and +1 V with successive addition of increasing phosphate concentrations ranging between 375 μM and 2000 μM into operational medium of the biosensor.…”

Section: Resultsmentioning

confidence: 99%

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

2019

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A biosensor based on conductive poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid) [Poly(Py‐co‐PyCOOH)] copolymer film coated gold electrode was developed for the quantitative phosphate determination. Enzyme pyruvate oxidase was immobilized chemically via the functional carboxylated groups of the copolymer. The potential to be applied which is deficiency of phosphate biosensor studies for precise phosphate detection was clarified by using differential pulse voltammetry technique. Performance of the sensing ability of the biosensor was improved by optimizing cofactor/cosubstrate concentrations, polymeric film density and pH. The biosensor showed a linearity up to phosphate concentration of 5 mM, operational stability with a relative standard deviation (RSD) of 0.07 % (n=7) and accuracy of 101 % at −0.15 V (vs. Ag/AgCl). Detection limit (LOD) and sensitivity were calculated to be 13.3 μM and 5.4 μA mM−1 cm−2, respectively by preserving 50 % of its initial response at the end of 30 days. It's performance was tested to determine phosphate concentrations in two streams of Zonguldak City in Turkey. Accuracy of phosphate measurement in stream water was found to be 91 %.

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“…Urea generates ammonium and bicarbonate ions under the action of urease, and the increase in ion concentration after the reaction enhances the current intensity generated by the electrode. By measuring the changing rate of electrode current intensity per unit time, the change of ion concentration in the solution can be calculated, thus the urease activity can be procured (Korkut et al, 2019). Carbon dioxide or phenol‐hypobromite was utilized as an electrode to determine the activity (Guilbault & Shu, 1972; Whitaker et al, 1965).…”

Section: Determination Methodsmentioning

confidence: 99%

A review of thermosensitive antinutritional factors in plant‐based foods

Kong

Liu

2022

Journal of Food Biochemistry

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Legumes and cereals account for the vast proportion of people's daily intake of plantbased foods. Meanwhile, a large number of antinutritional factors in legumes and cereals hinder the body absorption of nutrients and reduce the nutritional value of food.In this paper, the antinutritional effects, determination, and passivation methods of thermosensitive antinutritional factors such as trypsin inhibitors, urease, lipoxygenase, and lectin were reviewed to provide theoretical help to reduce antinutritional factors in food and improve the utilization rate of plant-based food nutrition. Since trypsin inhibitors and lectin have been more extensively studied and reviewed previously, the review mainly focused on urease and lipoxygenase. This review summarized the information of thermosensitive antinutritional factors, trypsin inhibitors, urease, lipoxygenase, and lectin, in cereals and legumes. The antinutritional effects, and physical and chemical properties of trypsin inhibitors, urease, lipoxygenase, and lectin were introduced. At the same time, the research methods for the detection and inactivation of these four antinutritional factors were also summarized in the order of research conducted time. The rapid determination and inactivation of antinutrients will be the focus of attention for the food industry in the future to improve the nutritional value of food. Exploring what structural changes could passivation technologies bring to antinutritional factors will provide a theoretical basis for further understanding the mechanisms of antinutritional factor inactivation.

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Electrochemical determination of urea using a gold nanoparticle-copolymer coated-enzyme modified gold electrode

Cited by 21 publications

References 35 publications

Discriminative detection of glucose and urea with a composite polymer nanofiber based matrix

Discriminative detection of glucose and urea with a composite polymer nanofiber based matrix

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

A review of thermosensitive antinutritional factors in plant‐based foods

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