Electrochemical determination of reduced glutathione (GSH) by applying the powder microelectrode technique

Chen, Jian; He, Zhongyu; Liu, Huan; Cha, Chuan-sin

doi:10.1016/j.jelechem.2006.01.008

Cited by 45 publications

(23 citation statements)

References 27 publications

(43 reference statements)

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“…The formal potential for glutathione oxidation at a Hg electrode has been found to be À380 mV (vs. SCE, pH 7.0) [37], which is equivalent at pH 3.6 to a formal potential of À144 mV (Ag/AgCl, 3 M Cl À ). Therefore, glutathione is, like SO 2 , a stronger reducing agent than the polyphenols.…”

Section: The Influence Of Glutathione On Polyphenol Cyclic Voltammogramsmentioning

confidence: 95%

Uncovering the influence of antioxidants on polyphenol oxidation in wines using an electrochemical method: Cyclic voltammetry

Makhotkina

Kilmartin

2009

Journal of Electroanalytical Chemistry

116

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Section: The Influence Of Glutathione On Polyphenol Cyclic Voltammogramsmentioning

confidence: 95%

Uncovering the influence of antioxidants on polyphenol oxidation in wines using an electrochemical method: Cyclic voltammetry

Makhotkina

Kilmartin

2009

Journal of Electroanalytical Chemistry

116

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“…The reduced form of glutathione (GSH) is required for maintaining the iron (II) form of hemoglobin in their reduced state and for maintaining the structure of red blood cells [4]. GSH is the main nonprotein thiol involved in the antioxidant cellular defense and most abundant low molecular mass thiol found in mammal cells [5]. It is a radioprotective agent [3] and the main sulphur compound in yeasts [6].…”

Section: Introductionmentioning

confidence: 99%

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

2012

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Several problems for the direct electrochemical oxidation of reduced glutathione (GSH) challenge the usage of electroanalytical techniques for its determination. In this work, the electrochemical oxidation of GSH catalyzed by gold nanoparticles electrodeposited on Nafion modified carbon paste electrode in 0.04 mol L(-1) universal buffer solution (pH 7.4) is proved successful. The effect of various experimental parameters including pH, scan rate and stability on the voltammetric response of GSH was investigated. At the optimum conditions, the concentration of GSH was determined using differential pulse voltammetry (DPV) in two concentration ranges: 0.1 × 10(-7) to 1.6 × 10(-5) mol L(-1) and 2.0 × 10(-5) to 2.0 × 10(-4) mol L(-1) with correlation coefficients 0.9988, 0.9949 and the limit of detections (LOD) are 3.9 × 10(-9) mol L(-1) and 8.2 × 10(-8) mol L(-1), respectively, which confirmed the sensitivity of the electrode. The high sensitivity, wide linear range, good stability and reproducibility, and the minimal surface fouling make this modified electrode useful for the determination of spiked GSH in urine samples and in tablet with excellent recovery results obtained.

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“…As a kind of abundant low molecular mass thiol found in mammals, reduced glutathione (GSH) is the main nonprotein thiol in cell involved in the antioxidant cellular defence with celluar concentration ranging from 0.5 to 10 mmol l −1 [20]. Numerous methods for the determination of GSH have been reported, for example, high-performance liquid chromatography [21,22], spectrofluorimetry [23], spectrophotometry [24] and potentiometry [25].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of ordered mesoporous carbon/cobalt oxide nanocomposite for determination of glutathione

Hou

Ndamanisha

Guo

et al. 2009

Electrochimica Acta

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Electrochemical determination of reduced glutathione (GSH) by applying the powder microelectrode technique

Cited by 45 publications

References 27 publications

Uncovering the influence of antioxidants on polyphenol oxidation in wines using an electrochemical method: Cyclic voltammetry

Uncovering the influence of antioxidants on polyphenol oxidation in wines using an electrochemical method: Cyclic voltammetry

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

Synthesis of ordered mesoporous carbon/cobalt oxide nanocomposite for determination of glutathione

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