Electrochemical Determination of Natural Drug Colchicine in Pharmaceuticals and Human Serum Sample and its Interaction with DNA

Abstract

Colchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification‐free boron‐doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19 V, and second one at around 1.37 V, in Britton‐Robinson buffer solution at pH 7.5. Wide dynamic range from 1 to 100 μM was obtained with a detection limit (3σintercept/slope) of a 0.26 μM, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time‐consuming and expensive separation methods.

Electroanalysis of Pharmaceuticals on Boron‐Doped Diamond Electrodes: A Review

“…Colchicine (COLC), a natural product used to treat gout, was quantified by Stankovic and coauthors using a DPV methodology . The authors used BR buffer at pH 7.5 on a bare BDD electrode.…”

Voltammetry as the First Method for Direct Determination of a Novel Antagonist of A2A Adenosine Receptors

“…Electrochemical methods, including voltammetry, are characterized by the high selectivity, sensitivity and a low cost of equipment. They are very often used for the determination of biologically active substances . To the best of our knowledge, no information about the analytical method for quantitative determination of IMT was described in the literature.…”

HPLC, Densitometric and Spectrophotometric Methods for the Simultaneous Determination of Colchicine and Probenecid in Their Binary Mixture

“…The literature review revealed that numerous techniques have been applied for the analysis of probenecid in a single dosage form such as HPLC [4][5][6][7], TLC [8][9], spectrophotometric [10][11][12][13], capillary electrophoresis [14][15] and spectrofluremetriy [16]. Also, various techniques were reported for the single determination of colchicine as HPLC [17][18][19][20], TLC [21][22][23], spectrophotometry [24][25] and electrochemistry [26][27][28]. Only two chromatographic methods [29,30] has been reported for the determination of both drugs in binary mixture.…”