Electrochemical determination of Cephalothin antibiotic by adsorptive stripping voltammetric technique

Al-Ghamdi, Ahmad; Alshadokhy, Mohammed A.; Al–Warthan, Abdulrahman

doi:10.1016/j.jpba.2004.02.034

Cited by 28 publications

(6 citation statements)

References 24 publications

(23 reference statements)

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“…The nature and acidity of the supporting buffer are some of the most important factors which strongly influence the stability of the analyte and its cathodic reduction as well as the adsorption processes [30]. Among the various investigated buffers (Britton-Robinson, acetate and phosphate) the best voltammetric signal in terms of sensitivity (peak height) and resolution (peak shape) have been available using Britton-Robinson buffer.…”

Section: Voltammetric Studies Of Cefepimementioning

confidence: 99%

Direct cefepime determination in human milk using solid mercury amalgam electrode manufactured with silver nanoparticles

Barbosa

Araújo

Trindade

et al. 2012

Journal of Electroanalytical Chemistry

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a b s t r a c tSolid silver mercury amalgam electrodes (AgSAEs) represent a suitable alternative to mercury electrodes due to their similar electrochemical properties and non-toxicity of the amalgam material. Based on this knowledge, we report the use of silver solid amalgam as working electrode to determine cefepime in human milk sample. Square-wave voltammogram registered for cefepime in Britton-Robinson buffer at pH 2.5, presented two well-defined reduction peaks, at À0.28 V (peak 1C) and À0.45 V (peak 2C). Under optimized condition, the calibration curves presented linear response from 3.0 Â 10 À7 to 2.1 Â 10 À6 mol L À1 with limits of detection and quantification of 8.51 Â 10 À9 and 2.84 Â 10 À8 mol L À1 , respectively. The applicability of the method was verified using spiked human milk samples and testing recovery percentages, which were ranged from 98.00% to 100.5%. The obtained results were also compared with those provided with the HPLC technique.

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Section: Voltammetric Studies Of Cefepimementioning

confidence: 99%

Direct cefepime determination in human milk using solid mercury amalgam electrode manufactured with silver nanoparticles

Barbosa

Araújo

Trindade

et al. 2012

Journal of Electroanalytical Chemistry

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“…There are many analytical techniques available to quantify cefazolin in a variety of human biological fluids, including plasma (total and unbound), urine, peritoneal dialysate, micro‐dialysate and serum (El‐Shaboury et al ., ). These techniques use liquid chromatography (LC) combined with ultra‐violet detection (Al‐Rawithi et al ., ; Baranowska et al ., ; Bayoumi et al ., ; Bompadre et al ., ; Chang et al ., ; Kamani et al ., ; Liang et al ., ; Martinez et al ., ; Murillo et al ., ; Nahata, ; Sorensen and Snor, ; Tsai and Chen, ; van Kralingen et al ., ; Wold and Turnipseed, ), visible spectrophotometric detection (El‐Shaboury et al ., ; Saleh et al ., ), tandem mass spectrometry (Daeseleire et al ., ; Hou et al ., ; Parker et al ., ) and spectro‐fluorometric methods (Yang et al ., ; Navarro et al ., ), chemi‐luminescence methods (Schulman et al ., ; Sun et al ., ) and electrochemical determination using adsorptive stripping voltammetry (Al‐ghamdi et al ., ). There are significantly fewer analytical techniques available to quantify cefalothin in human biological fluids; these are limited to total plasma (McWhinney et al ., ) and unbound plasma (Briscoe et al ., ), serum (Cooper et al ., ; Parker et al ., ; Wold and Turnipseed, ) and urine (Cooper et al ., ) using the techniques of LC combined with ultra‐violet detection (Cooper et al ., ; McWhinney et al ., ; Wold and Turnipseed, ) and tandem mass spectrometry (Parker et al ., ; Sime et al ., ; Zhang et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Determination of Cefalothin and Cefazolin in Human Plasma, Urine and Peritoneal Dialysate by UHPLC‐MS/MS: application to a pilot pharmacokinetic study in humans

Parker

Valero

Roberts

et al. 2015

Biomedical Chromatography

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An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the analysis of cefazolin and cefalothin in human plasma (total and unbound), urine and peritoneal dialysate has been developed and validated. Total plasma concentrations are measured following protein precipitation and are suitable for the concentration range of 1-500 µg/mL. Unbound concentrations are measured from ultra-filtered plasma acquired using Centrifree(®) devices and are suitable for the concentration range of 0.1-500 µg/mL for cefazolin and 1-500 µg/mL for cefalothin. The urine method is suitable for a concentration range of 0.1-20 mg/mL for cefazolin and 0.2-20 mg/mL for cefalothin. Peritoneal dialysate concentrations are measured using direct injection, and are suitable for the concentration range of 0.2-100 µg/mL for both cefazolin and cefalothin. The cefazolin and cefalothin plasma (total and unbound), urine and peritoneal dialysate results are reported for recovery, inter-assay precision and accuracy, and the lower limit of quantification, linearity, stability and matrix effects, with all results meeting acceptance criteria. The method was used successfully in a pilot pharmacokinetic study with patients with peritoneal dialysis-associated peritonitis, receiving either intraperitoneal cefazolin or cefalothin. Copyright © 2015 John Wiley & Sons, Ltd.

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“…Methods have been reported for determination of CF [9,10] or conjugated residues of CF [3] in milk. Electroanalytical techniques have been used to determine other cephalosporins, such as cefpodoxime proxetil [11], ceftazidime [12], cephalothin [13], cefminox [14], cefetamet-Na [15], cephalexin [16], cefaclor [17], ceftriaxone [18], cefixime [19], and cefepime [20], providing sensitivity equivalent to that of HPLC techniques. Most cephalosporins are electroactive by virtue of reduction of the (D 3 ) double bond of the cephem nucleus and possibly because of the presence of reducible substituents at the C 7 or C 3 positions in the molecule.…”

Section: Introductionmentioning

confidence: 99%

A simple method for electroanalytical determination of ceftiofur in UHT milk samples using square-wave voltammetry technique

et al. 2010

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A simple electroanalytical method was developed to determine the antibiotic ceftiofur (CF) in milk. The method is based on the adsorptive accumulation of the drug on a hanging mercury-drop electrode (HMDE) and the accompanying initiation of a negative square wave, which yielded well-defined cathodic peaks at -0.60 V (1C) and -0.91 V (2C) vs. Ag/AgCl. Calibration graphs were constructed and statistical parameters were evaluated. At pH 2.5, the square-wave voltammetry method revealed linearity from 52.4 to 524 ng mL -1 (r = 0.997), which is in accordance with the tolerance level of 100 ng mL -1 for CF as a residue in bovine milk established by the Food and Drug Administration (FDA) and the European Union. The limits of detection and quantification were 1.86 and 6.20 ng mL -1 , respectively. The method was tested to determine CF in spiked milk samples using HPLC as reference method.

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Electrochemical determination of Cephalothin antibiotic by adsorptive stripping voltammetric technique

Cited by 28 publications

References 24 publications

Direct cefepime determination in human milk using solid mercury amalgam electrode manufactured with silver nanoparticles

Direct cefepime determination in human milk using solid mercury amalgam electrode manufactured with silver nanoparticles

Determination of Cefalothin and Cefazolin in Human Plasma, Urine and Peritoneal Dialysate by UHPLC‐MS/MS: application to a pilot pharmacokinetic study in humans

A simple method for electroanalytical determination of ceftiofur in UHT milk samples using square-wave voltammetry technique

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