“…There are many analytical techniques available to quantify cefazolin in a variety of human biological fluids, including plasma (total and unbound), urine, peritoneal dialysate, micro‐dialysate and serum (El‐Shaboury et al ., ). These techniques use liquid chromatography (LC) combined with ultra‐violet detection (Al‐Rawithi et al ., ; Baranowska et al ., ; Bayoumi et al ., ; Bompadre et al ., ; Chang et al ., ; Kamani et al ., ; Liang et al ., ; Martinez et al ., ; Murillo et al ., ; Nahata, ; Sorensen and Snor, ; Tsai and Chen, ; van Kralingen et al ., ; Wold and Turnipseed, ), visible spectrophotometric detection (El‐Shaboury et al ., ; Saleh et al ., ), tandem mass spectrometry (Daeseleire et al ., ; Hou et al ., ; Parker et al ., ) and spectro‐fluorometric methods (Yang et al ., ; Navarro et al ., ), chemi‐luminescence methods (Schulman et al ., ; Sun et al ., ) and electrochemical determination using adsorptive stripping voltammetry (Al‐ghamdi et al ., ). There are significantly fewer analytical techniques available to quantify cefalothin in human biological fluids; these are limited to total plasma (McWhinney et al ., ) and unbound plasma (Briscoe et al ., ), serum (Cooper et al ., ; Parker et al ., ; Wold and Turnipseed, ) and urine (Cooper et al ., ) using the techniques of LC combined with ultra‐violet detection (Cooper et al ., ; McWhinney et al ., ; Wold and Turnipseed, ) and tandem mass spectrometry (Parker et al ., ; Sime et al ., ; Zhang et al ., ).…”