Electrochemical determination of BCR/ABL fusion gene based on in situ synthesized gold nanoparticles and cerium dioxide nanoparticles

Advanced applications of cerium oxide based nanozymes in cancer

“…For example, CeNPs can be used as a luminescent material to detect carcinoembryonic antigen (CEA), 17 improving the sensitivity of the immune sensor to detect tumour-specific growth factor (TSGF) through its catalytic properties 18 and be used for electrochemical biosensor detection of the BCR/ABL fusion gene. 19 According to the above illustrations, CeNPs are widely used in medicine, and they deserve further research and development. Table 1 summarises some examples of CeNPs applications in the detection of tumour markers.…”

“…Shenfeng Li et al developed an effective DNA electrochemical biosensor for the detection of BCR/ABL based on gold nanoparticles (GNP) synthesized in situ on the surface of multi-walled carbon nanotubes (MWCNT), CeO 2 - x and Chits composite membranes. 19 The detection of the BCR/ABL gene is of great significance for the early diagnosis, prognosis and assessment of chronic myelogenous leukaemia (CML) patients. 32 This method has been successfully used to detect real PCR samples with favourable selectivity, stability, and reproducibility.…”

Metal Oxide Nanoparticles in Electroanalysis

“…DNA detection is then performed after the target DNA is hybridized to the surface‐immobilized capture probes. Metal oxide nanoparticles function in such biosensors largely to increase the surface area for the immobilization of the capture probes 93–95, 99. This was demonstrated by Noorbaksh et al.…”

“…Many methods of immobilization can be employed to maximize the number of the capture probes on the surface of the electrode. Some examples include using the Au‐S bond with gold nanoparticles 93–95, using biotin‐avidin interaction 96, amide linkage through carboxylic groups on the surface of nanoparticles 97 or through silane reactions to form aldehyde groups on the electrode surface 98. DNA detection is then performed after the target DNA is hybridized to the surface‐immobilized capture probes.…”