Electrochemical detection system for supercritical fluid chromatography

A flow‐through column electrolytic cell for supercritical fluid chromatography*

“…A total 192 carbon strings were filled into a PTFE heat-shrinkable tube and heated by a hot air processing machine to prepare the column electrode. The column electrode was cut at 2 mm length for a working electrode and cut at 8 mm length for 19). Therefore, the total cell volume is 159 mm 3 .…”

“…In addition to the above‐mentioned detectors, a number of studies demonstrate a continued interest in SFC with electrochemical detection (ECD) for over two decades. A two‐electrode type wall‐jet electrochemical cell using a platinum microelectrode as a working electrode has been developed and 10 μg of ferrocene (5.4 × 10 –8 mol) has been successively detected . Dressman also developed an online electrochemical detector, which consisted of a 10 μm diameter platinum working electrode and a platinum tube quasi‐reference electrode, in which the working electrode and a fused‐silica capillary tube were sealed with epoxy resin.…”

Supercritical fluid chromatography with post‐column addition of supporting electrolyte solution for electrochemical determination of tocopherol and tocotrienol isomers

“…Detection in SFC is mainly performed by UV visible absorption [7] Article Related Abbreviations: ECD, electrochemical detection; FL, fluorescence detection; IS, internal standard; αT, α-tocopherol; αT3, α-tocotrienol; βT, β-tocopherol; βT3, β-tocotrienol; γT, γ-tocopherol; γT3, γ-tocotrienol; δT, δ-tocopherol; δT3, δ-tocotrienol and MS [8,9], but other detection methods have also been applied in SFC to enhance its performance and application. These detection methods include: fluorescence detection (FL) [10], refractive index detection [11], chemiluminescence detection [12], electrochemical detection (ECD) [13][14][15][16], ELSD [17,18], flame ionization detection [19], thermionic ionization detection [20], flame photometric detection [21], photoionization detection [22], and electron capture detection [23].…”

“…Detection in SFC is mainly performed by UV visible absorption [7] and MS [8, 9], but other detection methods have also been applied in SFC to enhance its performance and application. These detection methods include: fluorescence detection (FL) [10], refractive index detection [11], chemiluminescence detection [12], electrochemical detection (ECD) [13–16], ELSD [17, 18], flame ionization detection [19], thermionic ionization detection [20], flame photometric detection [21], photoionization detection [22], and electron capture detection [23].…”

“…In our experience, HPLC‐ECD is a sensitive and selective analytical system for the determination of redox compounds, becoming a useful detection method in HPLC [24]. In contrast, the application of ECD to SFC has been limited to a few studies [13–16, 25, 26]. This is mainly because of the lack of convenient electrochemical cells for the study of the electrochemical behavior of redox compounds in supercritical fluids.…”