Electrochemical detection of zeptomolar miRNA using an RNA-triggered Cu2+ reduction method

Kim, Hyo Yong; Song, Jayeon; Park, Hyun Gyu; Kang, Taejoon

doi:10.1016/j.snb.2022.131666

Cited by 29 publications

(12 citation statements)

References 57 publications

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“…The recently developed in vitro miRNA detection methods have been classified into three major groups, including those that detect biological recognition elements, use added micro-/nanomaterials, or use signal transduction/readout elements. Grouping biosensors can also focus on the actual detection methodology, including hybridization and amplification [ 178 ], colorimetric [ 108 ], fluorescent [ 109 ], optical [ 114 , 115 ], electrochemical [ 105 , 106 ], and magnetic nano-techniques [ 104 ].…”

Section: Discussionmentioning

confidence: 99%

miRNAs as Predictors of Barrier Integrity

et al. 2023

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The human body has several barriers that protect its integrity and shield it from mechanical, chemical, and microbial harm. The various barriers include the skin, intestinal and respiratory epithelia, blood–brain barrier (BBB), and immune system. In the present review, the focus is on the physical barriers that are formed by cell layers. The barrier function is influenced by the molecular microenvironment of the cells forming the barriers. The integrity of the barrier cell layers is maintained by the intricate balance of protein expression that is partly regulated by microRNAs (miRNAs) both in the intracellular space and the extracellular microenvironment. The detection of changes in miRNA patterns has become a major focus of diagnostic, prognostic, and disease progression, as well as therapy-response, markers using a great variety of detection systems in recent years. In the present review, we highlight the importance of liquid biopsies in assessing barrier integrity and challenges in differential miRNA detection.

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Section: Discussionmentioning

confidence: 99%

miRNAs as Predictors of Barrier Integrity

et al. 2023

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“…The synergistic coupling of the Cu 2+ reduction and miRNA recycling processes enabled the ultrasensitivity. The utility of the proposed technique was proven by accurately identifying the target miRNA in total RNA samples acquired from diverse cancer cell types [ 152 ].…”

Section: Electrochemical Biosensors Based On Nanostructured Materials...mentioning

confidence: 99%

A Comprehensive Review on Electrochemical Nano Biosensors for Precise Detection of Blood-Based Oncomarkers in Breast Cancer

Sadeghi

Naghib

et al. 2023

Biosensors

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Breast cancer (BC), one of the most common and life-threatening cancers, has the highest incidence rate among women. Early diagnosis of BC oncomarkers is considered the most effective strategy for detecting and treating BC. Finding the type and stage of BC in women as soon as possible is one of the greatest ways to stop its incidence and negative effects on medical treatment. The development of biosensors for early, sensitive, and selective detection of oncomarkers has recently attracted much attention. An electrochemical nano biosensor (EN) is a very suitable option for a powerful tool for cancer diagnosis. This comprehensive review provides information about the prevalence and pathobiology of BC, recent advances in clinically available BC oncomarkers, and the most common electrochemical nano biosensors for point-of-care (POC) detection of various BC oncomarkers using nanomaterial-based signal amplification techniques.

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“…MicroRNAs (miRNAs) are a sort of small noncoding and single-stranded RNA with the length of 18–25 nucleotides, which play critical roles in the regulation of gene expression at the translation level. − Many reported studies have shown that the abnormal or mutation expression of certain miRNAs is connected with the pathogenesis of some cancers, making them a valuable biomarker for early diagnosis of various cancers including lung, breast, colorectal, prostate, and so forth. − For instance, in a perspective view, miRNA-21 was reported to have overexpression in various cancer cell lines and was regarded to be closely related to the chemotherapeutic sensitivity of tumors. Thus, the development of analytical technologies that can accurately detect miRNA expression levels is very important for the early disease diagnosis and the monitoring of the therapeutic effect. ,, However, the highly efficient analysis of miRNAs is especially challenging due to their short length, facile degradation, low cellular abundance, and high sequence homology. , In the last several years, many efforts have been made to build biosensing platforms for miRNA assay, for example, fluorescence, , colorimetry, , electrochemiluminescence, − surface-enhanced Raman spectroscopy, , electrochemical , and photoelectrochemical (PEC) , methods, and so forth. Among these platforms, the PEC biosensor has attracted widespread attention due to its advantages of low background signal, excellent sensitivity and stability, especially the advantage of both optical and electrochemical methods by combining photoexcitation and electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

“…8,14,15 However, the highly efficient analysis of miRNAs is especially challenging due to their short length, facile degradation, low cellular abundance, and high sequence homology. 16,17 In the last several years, many efforts have been made to build biosensing platforms for miRNA assay, for example, fluorescence, 18,19 colorimetry, 20,21 electrochemiluminescence, 22−24 surface-enhanced Raman spectroscopy, 25,26 electrochemical 27,28 and photoelectrochemical (PEC) 29,30 methods, and so forth. Among these platforms, the PEC biosensor has attracted widespread attention due to its advantages of low background signal, excellent sensitivity and stability, especially the advantage of both optical and electrochemical methods by combining photoexcitation and electrochemical detection.…”

Section: ■ Introductionmentioning

confidence: 99%

Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

Jiang

et al. 2023

Anal. Chem.

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Sensitive and specific assay of microRNAs (miRNAs) is beneficial to early disease screening. Herein, we for the first time proposed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-mediated photoelectrochemical biosensors for the direct assay of miRNA-21. In this study, compared with traditional nucleic acid-based signal amplification strategies, the CRISPR/Cas13a system can greatly improve the specificity and sensitivity of target determination due to its accurate recognition and high-efficient trans-cleavage capability without complex nucleic acid sequence design. Moreover, compared with the CRISPR/Cas12a-based biosensing platform, the developed CRISPR/Cas13a-mediated biosensor can directly detect RNA targets without signal transduction from RNA to DNA, thereby avoiding signal leakage and distortion. Generally, the proposed biosensor reveals excellent analysis capability with a wider linear range from 1 fM to 5 nM and a lower detection limit of 1 fM. Additionally, it also shows satisfactory stability in the detection of human serum samples and cell lysates, manifesting that it has great application prospects in the areas of early disease diagnosis and biomedical research.

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Electrochemical detection of zeptomolar miRNA using an RNA-triggered Cu2+ reduction method

Cited by 29 publications

References 57 publications

miRNAs as Predictors of Barrier Integrity

miRNAs as Predictors of Barrier Integrity

A Comprehensive Review on Electrochemical Nano Biosensors for Precise Detection of Blood-Based Oncomarkers in Breast Cancer

Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

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