Electrochemical detection of uric acid and ascorbic acid: Application in serum

Choukairi, Mohamed; Bouchta, Dounia; Bounab, Loubna; atyah, M. Ben; Elkhamlichi, R.; Chaouket, F.; Raissouni, Ihssane; Rodríguez, Ignacio Naranjo

doi:10.1016/j.jelechem.2015.10.012

Cited by 47 publications

(19 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most papers addressing their electrochemical analysis showed the variation of AA oxidation current in the presence or the absence of UA . For those studying the influence of AA concentration on UA amperometric response, either the concentration of AA was kept constant and/or the AA concentration range adopted was generally high compared to that of UA . In such conditions it was therefore not possible to highlight any electrocatalytic effect even if the EC’ mechanism was effective.…”

Section: Resultsmentioning

confidence: 92%

See 1 more Smart Citation

New Insight into the EC’ Mechanism of Uric Acid Regeneration in the Presence of Ascorbic Acid on a Poly(3,4‐ethylenedioxithiophene) Modified Gold Electrode

et al. 2018

View full text Add to dashboard Cite

A gold electrode surface was functionalized by means of an electropolymerized conductive poly(3,4‐ethylenedioxythiophene) (PEDOT) organic layer. This modified electrode was used for the electrochemical detection of ascorbic (AA) and uric (UA) acids in an aqueous mixture with a selectivity around 340 mV. The electrochemical reactions kinetics were limited by AA diffusion and UA adsorption at the electrode surface, respectively. Following a previous study ([Electrochem Comm. 2011, 13, 423–425]) cyclic voltammetry was used to provide a better understanding of the EC’ mechanism of regeneration of UA by AA. Experiments particularly showed that allantoin (i. e. the final product of UA oxidation) is not actually involved in the synergic mechanism but rather the oxidized UA product diimine which is adsorbed at the electrode surface.

show abstract

Section: Resultsmentioning

confidence: 92%

“…Comparatively the UA oxidation peak shows a symmetrical shape as can be obtained in thin layer electrochemistry. All these results are in accordance with several previous works but surprisingly are in contradiction with others . The influence of UA concentration on UA anodic peak current was also investigated.…”

Section: Resultsmentioning

confidence: 99%

New Insight into the EC’ Mechanism of Uric Acid Regeneration in the Presence of Ascorbic Acid on a Poly(3,4‐ethylenedioxithiophene) Modified Gold Electrode

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The D was calculated as 7.634 × 10 −4 cm 2 ·s −1 , which was larger than in previous reports [ 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ].…”

Section: Resultsmentioning

confidence: 62%

Rapid Detection of Ascorbic Acid Based on a Dual-Electrode Sensor System Using a Powder Microelectrode Embedded with Carboxyl Multi-Walled Carbon Nanotubes

Zhang

2017

Sensors

View full text Add to dashboard Cite

In this paper, carboxyl groups were introduced by liquid oxidation methods onto multi-walled carbon nanotubes (MWCNTs) to improve the MWCNTs’ electrocatalytic properties. A platinum wire microelectrode (ME) was corroded using aqua regia and subsequently embedded with MWCNTs to achieve more active sites, producing a so-called powder microelectrode (PME). Compared with conventional MEs, the PME has a larger specific surface area and more active sites. When PME was used to detect ascorbic acid (AA), the AA oxidation potential shifted negatively and current peak was visibly increased. The calibration curve obtained for AA was in a range of 5.00 × 10−6~9.50 × 10−4 mol·L−1: Ipa(μA) = 3.259 × 10−2 + 1.801 × 102 C (mol·L−1) under the optimum testing conditions. Moreover, the detection and quantitation limits were confirmed at 4.89 × 10−7 mol·L−1 and 1.63 × 10−7 mol·L−1, respectively. When the fabricated PME was practically applied to detect AA, it was shown a recovery rate of 94~107% with relative standard deviation (RSD) <5%. The proposed strategy thus offers a promising, rapid, selective and low-cost approach to effective analysis of AA.

show abstract

“…One of the popular interferences that exist in human fluids is ascorbic acid (AA) which is present in body fluid at a much higher concentration than DOPAC . Uric acid (UA) is a strong reducer of free radicals and its high concentration levels in the body fluid leads to several diseases such as hyperuricemia and gout . Biologically, the simultaneous determination of DOPAC in presence of UA and AA is very crucial.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of β‐Cyclodextrin/Glycine/Carbon Nanotubes Electrochemical Neurotransmitters Sensor – Application in Ultra‐sensitive Determination of DOPAC in Human Serum

et al. 2018

View full text Add to dashboard Cite

In this work, an electrochemical sensor was constructed by applying two successive thin layers of glycine‐carbon nanotubes mixture and β‐cyclodextrin (CNTs‐Gly)/CD over glassy carbon electrode surface for some neurotransmitters determination. A host‐guest interaction between CD and neurotransmitters molecules is expected and resulted in enhanced sensitivity, selectivity and stability of sensor response. Other components of the sensor are crucial for the unique electrochemical response. Carbon nanotubes allowed large surface area for glycine distribution that provided hydrogen bonding to CD moieties and contributed to facilitated charge transfer. It was possible to determine 3,4‐dihydroxy phenyl acetic acid (DOPAC) in the linear range of 0.1 μmol L−1 to 80 μmol L−1 with detection limit of 9.40 nmol L−1, quantification limit of 31.5 nmol L−1 and sensitivity of 4.16 μA/μmol L−1. The proposed sensor was applied in synthetic cerebrospinal fluids samples using random standard addition method. Also, the proposed sensor was used to determine DOPAC in presence of common interferences and acceptable recovery results were achieved for its analysis in real blood serum. Figures of merit for (CNTs‐Gly)/CD composite in terms of precision, robustness, repeatability and reproducibility were reported.

show abstract

Electrochemical detection of uric acid and ascorbic acid: Application in serum

Cited by 47 publications

References 44 publications

New Insight into the EC’ Mechanism of Uric Acid Regeneration in the Presence of Ascorbic Acid on a Poly(3,4‐ethylenedioxithiophene) Modified Gold Electrode

New Insight into the EC’ Mechanism of Uric Acid Regeneration in the Presence of Ascorbic Acid on a Poly(3,4‐ethylenedioxithiophene) Modified Gold Electrode

Rapid Detection of Ascorbic Acid Based on a Dual-Electrode Sensor System Using a Powder Microelectrode Embedded with Carboxyl Multi-Walled Carbon Nanotubes

Fabrication of β‐Cyclodextrin/Glycine/Carbon Nanotubes Electrochemical Neurotransmitters Sensor – Application in Ultra‐sensitive Determination of DOPAC in Human Serum

Contact Info

Product

Resources

About