Electrochemical detection of the immobilization and hybridization of unlabeled linear and hairpin DNA on gold

Steichen, Marc; Buess‐Herman, C.

doi:10.1016/j.elecom.2005.02.015

Cited by 48 publications

(52 citation statements)

References 22 publications

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“…Moreover, EIS circumvents the labeling of at least one compound of the assay with, for example, enzymes, fluorophores, or redox reporters and can hence be considered as a label-free approach for detecting complementary recognition events at surfaces. [6][7][8][9] Therefore, EIS has been successfully applied for the detection of DNA conductivity, [10] DNA hybridization, [11][12][13][14][15] single-nucleotide mismatches in double-stranded DNA (dsDNA), [16] in situ hybridization of PNA/DNA, [17] DNA and RNA replication, [18,19] and the interaction of metal ions with DNA. [20,21] DNA detection by means of EIS is based on the repulsion between the DNA-modified electrode and a free-diffusing redox reporter, typically [Fe(CN) 6 ] 3À/4À .…”

Section: Introductionmentioning

confidence: 99%

Controlled Orientation of DNA in a Binary SAM as a Key for the Successful Determination of DNA Hybridization by Means of Electrochemical Impedance Spectroscopy

Gębala

Schuhmann

2010

ChemPhysChem

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Determination of DNA hybridization at electrode surfaces modified with thiol-tethered single-stranded DNA (ssDNA) capture probes and co-assembled with short-chain thiol derivatives using electrochemical impedance spectroscopy requires a careful design of the electrode/electrolyte interface as well as an in-depth understanding of the processes at the interface during DNA hybridization. The influence of the electrode potential, the ssDNA coverage, the ionic strength, the nature of the thiol derivative and especially the Debye length are shown to have a significant impact on the impedance spectra. A mixed monolayer comprising--in addition to the ssDNA capture probe--both mercaptohexanol (MCH) and mercaptopropionic acid (MPA) is suggested as an interface design which allows a high efficiency of the DNA hybridization concomitantly with a reliable modulation of the charge-transfer resistance of the electrode upon hybridization.

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Section: Introductionmentioning

confidence: 99%

Controlled Orientation of DNA in a Binary SAM as a Key for the Successful Determination of DNA Hybridization by Means of Electrochemical Impedance Spectroscopy

Gębala

Schuhmann

2010

ChemPhysChem

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“…Moreover, as it is well known that the mole ratio can influence probe density, [17] different proportions of DNA and MCB were chosen while keeping a constant total thiol concentration (DNA + MCB) of 50 mm. [28] Experimental Section…”

Section: Introductionmentioning

confidence: 99%

“…Various immobilization procedures were considered (Scheme 1): 1) a one-step adsorption procedure, in which the DNA probe and the diluent were assembled together [14,28,29] (hereafter procedure P1 or co-adsorption procedure); 2) the conventional two-step adsorption method, in which DNA is immobilized first (hereafter referred to as procedure P2 or classical two-step procedure); 3) the reversed two-step procedure, in which the diluent is adsorbed first (hereafter procedure P3). The DNA sequence was an 18-mer oligonucleotide modified by an alkylthiol chain in the 5' end for the formation of a covalent bond with the gold surface, and by a fluorescent boron-dipyrromethene (BODIPY) dye in the 3' end for spectroelectrochemical measurements.…”

Section: Introductionmentioning

confidence: 99%

In Situ Fluorescence Microscopy Study of the Interfacial Inhomogeneity of DNA Mixed Self‐Assembled Monolayers at Gold Electrodes

et al. 2015

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Mixed self‐assembled monolayers composed of a fluorescently labeled DNA and a mercaptobutanol diluent immobilized on gold electrodes were characterized by electrochemical measurements coupled with in situ fluorescence microscopy. The reductive desorption of the self‐assembled monolayers was monitored in real time through variations in the capacitance and fluorescence intensity. Desorption occurred in several steps, which was related to substrate crystallinity. Fluorescence microscopy revealed the presence of spatial heterogeneities in the form of highly fluorescent aggregates that remained at the electrode surface even after a reductive desorption step. This in situ electrofluorescence microscopy technique is useful to optimize the formation of the mixed layer to obtain a homogeneous distribution of the probes, which thus improves the efficiency of the recognition process in the development of biosensors.

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“…Taken together, the most common hairpin probe immobilization strategy is the self-assembled monolayers (SAMs) technology because of the ease of fabrication, and reasonable reproducibility, storage stability and regeneration of resulting sensing phases [19]. Chronocoulometry studies showed that lower surface density is achieved using thiolated hairpin compared to linear ssDNA under the same deposition conditions on gold electrodes [20].…”

Section: Probe Immobilizationmentioning

confidence: 99%

Structured Nucleic Acid Probes for Electrochemical Devices

Miranda‐Castro¹,

de‐los‐Santos‐Álvarez²,

Lobo-Castañón³

et al. 2009

Electroanalysis

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The use of nucleic acid with a specific sequence and a highly ordered secondary structure such as hairpins, quadruplexes and pseudoknots as biological recognition elements and switches in biosensors is rapidly increasing because of their improved features (e.g. selectivity) when compared with the traditional linear probes. Owing to the novelty, a critical outlook of their characteristics and a compilation of the latest advances are lacking. This article describes the potential of those nucleic acids probes whose molecular recognition ability relies on a conformational change (e.g. folding/unfolding mechanism) in electrochemical sensing. It provides an overview of the toolbox of assays using these probes for genosensors and aptasensors, highlighting its performance characteristics and the prospects and challenges for biosensor design.

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Electrochemical detection of the immobilization and hybridization of unlabeled linear and hairpin DNA on gold

Cited by 48 publications

References 22 publications

Controlled Orientation of DNA in a Binary SAM as a Key for the Successful Determination of DNA Hybridization by Means of Electrochemical Impedance Spectroscopy

Controlled Orientation of DNA in a Binary SAM as a Key for the Successful Determination of DNA Hybridization by Means of Electrochemical Impedance Spectroscopy

In Situ Fluorescence Microscopy Study of the Interfacial Inhomogeneity of DNA Mixed Self‐Assembled Monolayers at Gold Electrodes

Structured Nucleic Acid Probes for Electrochemical Devices

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