Electrochemical detection of microcystin-LR based on its deleterious effect on DNA

Zhang, Keke; Ma, Huiying; Yan, Ping; Tong, Wenjun; Huang, Xiaohua; Chen, David D. Y.

doi:10.1016/j.talanta.2018.03.051

Cited by 21 publications

(10 citation statements)

References 36 publications

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“…At present, enzyme based biosensors (including optical and electrochemical biosensors), immunosensors (including electrochemical, piezoelectric, NMR-based and optical immunosensors such as Surface Plasmon Resonance (SPR) immunosensors, Evanescent Wave Fiber-optic immunosensors, Luminescent immunosensors, Fluorescent immunosensors, and Immunoarray biosensors) and nucleic acid biosensors (including electrochemical DNA and SPR-DNA biosensor) have been developed for successful MCs determination [ 151 , 152 , 153 , 156 ]. A rapid and sensitive SPR biosensor, which incorporates commercial Adda-group antibody (Ab) and has the capacity for broader recognition of various MC variants than earlier developed sensors for BGA supplements was established and validated.…”

Section: Biosensor Methods To Detect Microcystinsmentioning

confidence: 99%

“…The constructed electrochemical biosensor that possesses good stability against other components in natural water sample, prepared via physically immobilizing calf thymus DNA (ctDNA) on gold electrode after characterizing the deleterious effect of MC-LR to ctDNA, was utilized to detect MC-LR in local water bodies. The technique indicated linear range of 4–512 ng/L, LOD of 1.4 ng/L (perceived to be 700-fold lower than WHO’s suggested guideline value) and recoveries were 95.1% to 107.6% [ 156 ]. A novel fiber optical chemiluminescent biosensor (FOCB) system was successfully generated utilizing fiber optic bio-probe as biorecognition element as well as transducer and a Si-based photodiode detector (PD-3000).…”

Section: Biosensor Methods To Detect Microcystinsmentioning

confidence: 99%

See 1 more Smart Citation

A Mini-Review on Detection Methods of Microcystins

Massey

Jia

et al. 2020

Toxins

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Cyanobacterial harmful algal blooms (CyanoHABs) produce microcystins (MCs) which are associated with animal and human hepatotoxicity. Over 270 variants of MC exist. MCs have been continually studied due of their toxic consequences. Monitoring water quality to assess the presence of MCs is of utmost importance although it is often difficult because CyanoHABs may generate multiple MC variants, and their low concentration in water. To effectively manage and control these toxins and prevent their health risks, sensitive, fast, and reliable methods capable of detecting MCs are required. This paper aims to review the three main analytical methods used to detect MCs ranging from biological (mouse bioassay), biochemical (protein phosphatase inhibition assay and enzyme linked immunosorbent assay), and chemical (high performance liquid chromatography, liquid chromatography-mass spectrometry, high performance capillary electrophoresis, and gas chromatography), as well as the newly emerging biosensor methods. In addition, the current state of these methods regarding their novel development and usage, as well as merits and limitations are presented. Finally, this paper also provides recommendations and future research directions towards method application and improvement.

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Section: Biosensor Methods To Detect Microcystinsmentioning

confidence: 99%

Section: Biosensor Methods To Detect Microcystinsmentioning

confidence: 99%

A Mini-Review on Detection Methods of Microcystins

Massey

Jia

et al. 2020

Toxins

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“…3D graphene-based biosensor MC-LR Abs 0.05 20 0.05 [110] Electrochemical biosensor Calf thymus DNA 0.004 0.512 0.0014 [111] Electrochemical immunoassay sensor Alkyne end-terminated Ab 0.01 200 0.004 [112] Electrochemical immunosensor Antigen of MC-LR 0.005 50 0.004 [113] Photoelectrochemical immunoassay sensor Ab of MC-LR 0.005 500 0.001 [114] Electrochemical immunosensor Ab of MC-LR 0.01 20 0.005 [115] Electrochemical impedance biosensor MC-LR aptamer 0.049 99.5 0.017 [116] Photoelectrochemical aptasensor MC-LR aptamer NA NA 1.691 × 10 −5 [ 117] Colorimetric sensor MC-LR aptamer 0.4 7462.5 0.368 [118] Fluorescence aptasensor MC-LR aptamer 0.01 50 0.002 [119] Fluorescence sensor MC-LR aptamer 0.398 1194 0.137 [119] Colorimetric sensor MC-LR aptamer NA NA 0.497 [120] Photoelectrochemical aptasensor Single-stranded DNA aptamer of MC-LR 0.01 5 5 × 10 −9 [ 122] Electrochemical aptasensor Thiolated MC-LR aptamers 5 × 10 −6 3 × 10 −2 2 × 10 −6 [ 123] Abbreviations: 3D, three-dimensional; Ab, antibody; MC, microcystin; NA, not available; LOD, limit of detection (the minimal amount of toxin whose presence or absence can be detected); LOQ, limit of quantification (upper and lower limits of the method are extremes of the linear range of the method); SPR, surface plasmon resonance; SWNT, single wall nanotubes.…”

Section: Protein Phosphate Inhibition Assaymentioning

confidence: 99%

“…A detection limit of 0.05 µg/L was achieved with a linear correlation over a range of 0.05‐20 µg/L MC‐LR. [ 110 ] Zhang et al [ 111 ] have developed an electrochemical biosensor using the effect of MC‐LR on DNA. They have immobilized calf thymus DNA (ctDNA) on a gold electrode.…”

Section: Approaches For Detection Of Mcsmentioning

confidence: 99%

Recent developments in the methods of quantitative analysis of microcystins

Kumar

Rautela

Kesari

et al. 2020

J Biochem & Molecular Tox

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Cyanotoxins are produced by the toxic cyanobacterial species present in algal blooms formed in water bodies due to nutrient over-enrichment by human influences and natural environmental conditions. Extensive studies are available on the most widely encountered cyanotoxins, microcystins (MCs) in fresh and brackish water bodies. MC contaminated water poses severe risks to human health, environmental sustainability, and aquatic life. Therefore, commonly occurring MCs should be monitored. Occasionally, detection and quantification of these toxins are difficult due to the unavailability of pure standards. Enzymatic, immunological assays, and analytical techniques like protein phosphatase inhibition assay, enzymelinked immunosorbent assay, high-performance liquid chromatography, liquid chromatography-mass spectrometry, and biosensors are used for their detection and quantification. There is no single method for the detection of all the different types of MCs; therefore, various techniques are often combined to yield reliable results. Biosensor development offered a problem-solving approach in the detection of MCs due to their high accuracy, sensitivity, rapid response, and portability. In this review, an endeavor has been made to uncover emerging techniques used for the detection and quantification of the MCs.

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“…In this case, the use of biosensors greatly simplifies the task of such control. A high number of recent publications and reviews reports applications of different biosensors for detection of toxins and cyanotoxins in water using various biorecognition elements and transducers supports [19][20][21][22][23][24][25][26]. Nevertheless, the performance of these biosensors is focused on the detection of cyanotoxins and not the cyanobacteria cells.…”

Section: Introductionmentioning

confidence: 99%

3D Impedimetric Biosensor for Cyanobacteria Detection in Natural Water Sources

et al. 2021

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The excessive growth of cyanobacteria in freshwater sources produces the development of toxic blooms mainly due to the production of cyanotoxins. Here, a novel impedimetric biosensor based on a three-dimensional interdigitated electrode array (3D-IDEA) for detection of cyanobacteria cells is reported. The 3D-IDEA sensor surface biofunctionalization was performed by means of the layer-by-layer method using polyethyleneimine (PEI) as the anchoring layer and concanavalin A (Con A) as the bioreceptor to lipopolysaccharides of cyanobacteria cells. The developed PEI-Con A 3D-IDEA sensors show a linear response (R2 = 0.992) of the impedance changes (RS) versus the logarithm of cyanobacteria concentrations in the range of 102–105 cells·mL−1 with the detection limit of 100 cells·mL−1. Moreover, to prevent the interference from components that may be present in real water samples and minimize a possible sample matrix effect, a filtration methodology to recover cyanobacterial cells was developed. The proposed methodology allows 91.2% bacteria recovery, permitting to obtain results similar to controlled assays. The developed system can be used in aquatic environments to detect cyanobacteria and consequently to prevent the formation of blooms and the production of cyanotoxins. Con A can bind to most polysaccharides and so react with other types of bacteria. However, currently, on the market, it is not possible to find specific biorecognition elements for cyanobacteria. Taking into consideration the specificity of samples to be analyzed (natural water resources), it is difficult to expect high concentration of other bacteria. In this sense, the developed methodology may be used as an alarm system to select samples for more thorough and precise laboratory analysis.

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Electrochemical detection of microcystin-LR based on its deleterious effect on DNA

Cited by 21 publications

References 36 publications

A Mini-Review on Detection Methods of Microcystins

A Mini-Review on Detection Methods of Microcystins

Recent developments in the methods of quantitative analysis of microcystins

3D Impedimetric Biosensor for Cyanobacteria Detection in Natural Water Sources

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