Electrochemical detection of human epidermal growth factor receptor 2 using an aptamer on cobalt phthalocyanines – Cerium oxide nanoparticle conjugate

Centane, Sixolile; Mgidlana, Sithi; Openda, Yolande Ikala; Nyokong, Tebello

doi:10.1016/j.bioelechem.2022.108146

Cited by 12 publications

(4 citation statements)

References 40 publications

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“…In addition to polymers, dendrimers, and SAMs, we can highlight the use of metalorganic frameworks (MOFs) [132][133][134][135], as well as metal complexes of porphyrins [136] and phthalocyanines [137,138].…”

Section: Metal Complexes Of Porphyrins Phthalocyanines and Metal-orga...mentioning

confidence: 99%

“…Centane et al [137] developed a biosensor for human epidermal growth factor receptor 2 (HER2) and studied the use of cobalt tetraphenylacetic acid phthalocyanine and cobalt tetraphenylpropionic acid phthalocyanine, which, after activation in DCC/NHS, were used as a platform to immobilize the HB5 aptamer. Also, to increase the specific area of the sensor, phthalocyanines were associated with nanoparticles based on cerium oxide.…”

Section: Metal Complexes Of Porphyrins Phthalocyanines and Metal-orga...mentioning

confidence: 99%

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Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Section: Metal Complexes Of Porphyrins Phthalocyanines and Metal-orga...mentioning

confidence: 99%

Section: Metal Complexes Of Porphyrins Phthalocyanines and Metal-orga...mentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

View full text Add to dashboard Cite

show abstract

“…Similarly, a non-enzymatic glucose sensor was developed using a straw sheaf-like CONPs-modified electrode, which showed a high sensitivity of 669 µA mM −1 cm 2 with a LOD of 0.31 µM [ 84 ]. Centane and colleagues developed an electrochemical aptasensor by immobilizing the aptamer on cobalt phthalocyanines—cerium oxide (CeO) NP conjugate for the sensing of human epidermal growth factor receptor 2 (HER) [ 85 ]. Here, the aptamer HB5 was covalently linked to the modified electrode using DCC/NHS coupling via amide bond formation between the free amine groups of HB5 and free carboxylic acid groups of the conjugate.…”

Section: Various Nanomaterial-modified Electrodesmentioning

confidence: 99%

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Theyagarajan

Kim

2023

Biosensors

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Electrochemical biosensors are superior technologies that are used to detect or sense biologically and environmentally significant analytes in a laboratory environment, or even in the form of portable handheld or wearable electronics. Recently, imprinted and implantable biosensors are emerging as point-of-care devices, which monitor the target analytes in a continuous environment and alert the intended users to anomalies. The stability and performance of the developed biosensor depend on the nature and properties of the electrode material or the platform on which the biosensor is constructed. Therefore, the biosensor platform plays an integral role in the effectiveness of the developed biosensor. Enormous effort has been dedicated to the rational design of the electrode material and to fabrication strategies for improving the performance of developed biosensors. Every year, in the search for multifarious electrode materials, thousands of new biosensor platforms are reported. Moreover, in order to construct an effectual biosensor, the researcher should familiarize themself with the sensible strategies behind electrode fabrication. Thus, we intend to shed light on various strategies and methodologies utilized in the design and fabrication of electrochemical biosensors that facilitate sensitive and selective detection of significant analytes. Furthermore, this review highlights the advantages of various electrode materials and the correlation between immobilized biomolecules and modified surfaces.

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“…These elements have unique properties such as control structures, excellent physical and chemical properties, and a unique unpaired electronic configuration of 4f orbitals, making them exciting candidates for developing new electrochemical sensors [ 21 , 22 ]. REEs act as catalysts but also contribute to high conductivity and favorable optical and thermal properties [ 23 , 24 ]. Functional nanomaterials based on cerium, Ce (the most abundant REE), are characterized by high catalytic effects, thermal and chemical stability, semiconductor capabilities, large-scale oxygen storage potential, and eco-friendly properties [ 8 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Triangle-Shaped Cerium Tungstate Nanoparticles Used to Modify Carbon Paste Electrode for Sensitive Hydroquinone Detection in Water Samples

Stanković,

Đurđić,

Ognjanović

et al. 2024

Sensors

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In this study, we propose an eco-friendly method for synthesizing cerium tungstate nanoparticles using hydrothermal techniques. We used scanning, transmission electron microscopy, and X-ray diffraction to analyze the morphology of the synthesized nanoparticles. The results showed that the synthesized nanoparticles were uniform and highly crystalline, with a particle size of about 50 nm. The electrocatalytic properties of the nanoparticles were then investigated using cyclic voltammetry and electrochemical impedance spectroscopy. We further used the synthesized nanoparticles to develop an electrochemical sensor based on a carbon paste electrode that can detect hydroquinone. By optimizing the differential pulse voltammetric method, a wide linearity range of 0.4 to 45 µM and a low detection limit of 0.06 µM were obtained. The developed sensor also expressed excellent repeatability (RSD up to 3.8%) and reproducibility (RSD below 5%). Interferences had an insignificant impact on the determination of analytes, making it possible to use this method for monitoring hydroquinone concentrations in tap water. This study introduces a new approach to the chemistry of materials and the environment and demonstrates that a careful selection of components can lead to new horizons in analytical chemistry.

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Electrochemical detection of human epidermal growth factor receptor 2 using an aptamer on cobalt phthalocyanines – Cerium oxide nanoparticle conjugate

Cited by 12 publications

References 40 publications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Triangle-Shaped Cerium Tungstate Nanoparticles Used to Modify Carbon Paste Electrode for Sensitive Hydroquinone Detection in Water Samples

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