Electrochemical detection of DNA hybridization using methylene blue and electro-deposited zirconia thin films on gold electrodes

Zhu, Ning; Zhang, Aiping; Wang, Qingjiang; He, Pingang; Fang, Yuzhi

doi:10.1016/j.aca.2004.01.017

Cited by 134 publications

(70 citation statements)

References 32 publications

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“…The biosensor was seven orders of magnitude wider in a linear range, and the sensitivity of the biosensor was higher over those in our previous work [21,32] and other biosensors (Table 1). [33][34][35][36][37][38] Figure 7 (A) DPVs of reduction peak of MB at probe-modified electrode (a) and hybridization with different concentrations of the target sequence:…”

Section: Analytical Performancementioning

confidence: 99%

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

2012

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In this work, a sensitive electrochemical DNA biosensor for the detection of sequence-specific target DNA was reported. Firstly, CuO nanospindles (CuO NS) were immobilized on the surface of a glassy carbon electrode (GCE). Subsequently, gold nanoparticles (Au NPs) were introduced to the surface of CuO NS by the electrochemical deposition mode. Probe DNA with SH (HS-DNA) at the 5'-phosphate end was covalently immobilized on the surface of the Au NPs through Au-S bond. Scanning electron microscopy (SEM) was used to elucidate the morphology of the assembled film, and electrochemical impedance spectroscopy technique (EIS) was used to investigate the DNA sensor assembly process. Hybridization detection of DNA was performed with differential pulse voltammetry (DPV) and the methylene blue (MB) was hybridization indicator. Under the optimal conditions, the decline of reduction peak current of MB (ΔI) was linear with the logarithm of the concentration of complementary DNA from 1.0× 10 -13 to 1.0×10 -6 mol•L -1 with a detection limit of 3.5×10 -14 mol•L -1 (S/N＝3). In addition, this DNA biosensor has good selectivity, and even can distinguish single-mismatched target DNA.

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Section: Analytical Performancementioning

confidence: 99%

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

2012

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“…Electrochemically deposited ZrO 2 film onto gold surface has been used to immobilize ssDNA specific to Mycobacterium tuberculosis by utilizing affinity between the oxygen atom of phosphate group and zirconium to fabricate DNA biosensor [15,16]. The electrochemically deposited nanoZrO 2 film and ZrO 2 /carbon paste electrode have also been recently used for development of DNA biosensors [17].…”

Section: Introductionmentioning

confidence: 99%

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

et al. 2011

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O1 gene based 24-mer single stranded deoxyribonucleic acid probe (ssDNA) has been immobilized onto sol-gel derived nanostructured zirconium oxide (NanoZrO 2 ) film fabricated onto indium-tin-oxide (ITO) coated glass plate to detect Vibrio cholerae. The X-ray diffraction and Atomic Force Microscopy techniques have been used to characterize the nanostructured ZrO 2 (particle size of~30-40 nm) and the ssDNA/ZrO 2 bioelectrode. The hybridization of ssDNA/ZrO 2 bioelectrode with the complementary and genomic DNA has been investigated using differential pulse voltammetry. The results of electrochemical studies suggest that electro-active and cationic NanoZrO 2 provides an effective surface to bind with the phosphate group of DNA resulting in enhanced electron transport. The ssDNA/NanoZrO 2 bioelectrode shows a detection range from 1 10 À8 to 10 nM of complementary DNA of V. cholerae within 60 s of hybridization time at 25 8C using methylene blue as an electroactive indicator. This O1 gene based metal oxide (ZrO 2 ) sensor exhibits sensitivity for ssDNA/NanoZrO 2 /ITO bioelectrode as 0.48 mA/nM cm À2 for complementary DNA and 2.34 mA/nM cm À2 for genomic DNA with regression coefficients (R) of 0.991 and 0.995, respectively. This DNA bioelectrode is stable for about 15 weeks when stored at 4 8C.

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“…[1][2][3][4][5] Different forms of electrochemical DNA biosensors have been developed relying on the sequence-specific hybridization of nucleic acids and such hybridization events could be detected directly 6,7 or by DNA intercalator. [8][9][10][11][12] Amongst, the later fashion using an intercalator as redox label or indicator for DNA hybridization attracts great interest. In a typical assay, the intercalator interacts with the DNA duplex but not with a single strand of DNA.…”

Section: Introductionmentioning

confidence: 99%

Nucleic Acid Biosensor for Detection of Human Immunodeficiency Virus Using Aquabis(1,10‐phenanthroline)copper(II) Perchlorate as Electrochemical Indicator

Niu¹,

Wang

2008

Chin. J. Chem.

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Electrochemical detection of DNA hybridization using methylene blue and electro-deposited zirconia thin films on gold electrodes

Cited by 134 publications

References 32 publications

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

Nucleic Acid Biosensor for Detection of Human Immunodeficiency Virus Using Aquabis(1,10‐phenanthroline)copper(II) Perchlorate as Electrochemical Indicator

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