Electrochemical detection of C-reactive protein using Copper nanoparticles and hybridization chain reaction amplifying signal

Zhang, Wenjuan; Wang, Junchun; Zhang, Yuzhong

doi:10.1016/j.ab.2017.09.017

Cited by 44 publications

(11 citation statements)

References 23 publications

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“…Nanoparticle genotoxicity in plants could be caused by direct NPs intercalation in DNA strand or indirect by releasing free ions which can cause DNA damage (Kruszewski et al 2011). For example, Cu NPs (Zhang et al 2017) or Au NPs (Baetsen-Young et al 2018) can intercalate into the DNA strand. It is not yet known whether Fe 3 O 4 NPs are able to intercalating between DNS strands, but we suppose that released Fe 2+ or Fe 3+ ions (Mahdavi et al 2013) could directly affect DNA.…”

Section: Rapd Analysismentioning

confidence: 99%

Impact of iron oxide nanoparticles on yellow medick (Medicago falcata L.) plants

Kokina

Plaksenkova

Jermaļonoka

et al. 2020

Journal of Plant Interactions

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A large number of studies have explored the effects of various nanoparticles (NPs) on different economically important plant species. In this study, yellow medick plants were grown for five weeks using hydroponics with the addition of Fe 3 O 4 NPs at 1, 2 and 4 mg/L. Plant morphology, chlorophyll a, genotoxicity and expression of miR159c, one of the most important plant miRNA that is involved in plant response to fungal infections, were investigated. The results indicated that Fe 3 O 4 NPs significantly increased plant root length (9%-32%), chlorophyll a fluorescence (1.94-2.8fold), miRNA expression (0.31-0.42-fold), induced genotoxicity and reduced genome stability (12.5%-13.3%), compared to those of the control. The study demonstrated that Fe 3 O 4 NPs simultaneously induce genome instability in yellow medick and increase expression of miR159c. Therefore, Fe 3 O 4 NPs can be used to increase plant resistance to fungal diseases, such as powdery mildew.

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Section: Rapd Analysismentioning

confidence: 99%

Impact of iron oxide nanoparticles on yellow medick (Medicago falcata L.) plants

Kokina

Plaksenkova

Jermaļonoka

et al. 2020

Journal of Plant Interactions

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“…Enhancing the detection sensitivity of ELISA microplates is essential for increasing the capture antibody-coated surface area or providing a homogeneous orientation of the capture antibodies. Microplates with high amounts of surface area, such as gold nanoarray-based microplates 30–34 or polystyrene bead-based microplates 35–37 , provide a greater amount coating area for the capture antibodies, thus enhancing their detection sensitivity. However, the orientation of capture antibodies coated on such microplates is heterogeneous due to the random interaction of the polystyrene surface with any portion of the capture antibodies, and this heterogenous orientation impairs the binding of the capture antibodies and antigens 38 .…”

Section: Discussionmentioning

confidence: 99%

Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates

Chen

Hsieh

et al. 2018

Sci Rep

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The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells on microplates to improve the coating amount and displayed orientation of capture antibodies. One or eight repeated fragment crystallisable (Fc) binding domains of protein G are stably expressed on the surface of BALB/c 3T3 cells (termed 1pG cells or 8pG cells), which then act as highly antibody-trapping microparticles. The 8pG cells showed higher antibody-trapping ability than the 1pG cells did. The antibody-coating amount of the 8pG cell-based microplates was 1.5–23 times and 1.2–6.8 times higher than that of traditional polystyrene-based and commercial protein G-based microplates, respectively. The 8pG cell-based microplates were then applied to an anti-IFN-α sandwich ELISA and an anti-CTLA4 competitive ELISA, respectively, and dramatically enhanced their detection sensitivity. Importantly, direct coating unpurified capture antibody produced by mammalian cells did not impair the antigen-capturing function of 8pG cell-based microplates. The 8pG cell-based microplates exhibited a significant improvement in antibody-coating amount and preserved the homogeneous orientation of capture antibodies, making them a potential replacement for traditional microplates in various formats of ELISAs.

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“…Electrochemical mechanisms have been developed for other potential MDD biomarker such as CRP. Two sandwich-type electrochemical immunoassays have been developed for serum CRP detection [ 57 , 58 ]. A CRP-selective sandwich immunosensor was fabricated using copper nanoparticles as a signaling molecule coupled with a hybridization chain reaction to amplify the resultant signal [ 58 ].…”

Section: Suggested Electrochemical Point-of-care Approachesmentioning

confidence: 99%

“…Two sandwich-type electrochemical immunoassays have been developed for serum CRP detection [ 57 , 58 ]. A CRP-selective sandwich immunosensor was fabricated using copper nanoparticles as a signaling molecule coupled with a hybridization chain reaction to amplify the resultant signal [ 58 ]. The copper-based sensor achieved proof-of-concept testing for sensitive and selective detection of CRP in the presence of interfering compounds (AFP, CEA, L-Cys, lysine, uric acid) [ 58 ].…”

Section: Suggested Electrochemical Point-of-care Approachesmentioning

confidence: 99%

The emergence of psychoanalytical electrochemistry: the translation of MDD biomarker discovery to diagnosis with electrochemical sensing

et al. 2022

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The disease burden and healthcare costs of psychiatric diseases along with the pursuit to understand their underlying biochemical mechanisms have led to psychiatric biomarker investigations. Current advances in evaluating candidate biomarkers for psychiatric diseases, such as major depressive disorder (MDD), focus on determining a specific biomarker signature or profile. The origins of candidate biomarkers are heterogenous, ranging from genomics, proteomics, and metabolomics, while incorporating associations with clinical characterization. Prior to clinical use, candidate biomarkers must be validated by large multi-site clinical studies, which can be used to determine the ideal MDD biomarker signature. Therefore, identifying valid biomarkers has been challenging, suggesting the need for alternative approaches. Following validation studies, new technology must be employed to transition from biomarker discovery to diagnostic biomolecular profiling. Current technologies used in discovery and validation, such as mass spectroscopy, are currently limited to clinical research due to the cost or complexity of equipment, sample preparation, or measurement analysis. Thus, other technologies such as electrochemical detection must be considered for point-of-care (POC) testing with the needed characteristics for physicians’ offices. This review evaluates the advantages of using electrochemical sensing as a primary diagnostic platform due to its rapidity, accuracy, low cost, biomolecular detection diversity, multiplexed capacity, and instrument flexibility. We evaluate the capabilities of electrochemical methods in evaluating current candidate MDD biomarkers, individually and through multiplexed sensing, for promising applications in detecting MDD biosignatures in the POC setting.

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Electrochemical detection of C-reactive protein using Copper nanoparticles and hybridization chain reaction amplifying signal

Cited by 44 publications

References 23 publications

Impact of iron oxide nanoparticles on yellow medick (Medicago falcata L.) plants

Impact of iron oxide nanoparticles on yellow medick (Medicago falcata L.) plants

Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates

The emergence of psychoanalytical electrochemistry: the translation of MDD biomarker discovery to diagnosis with electrochemical sensing

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