Electrochemical detection of avian influenza virus H5N1 gene sequence using a DNA aptamer immobilized onto a hybrid nanomaterial-modified electrode

Biosensors for waterborne viruses: Detection and removal

“…This electrode provided a porous structure with a large effective surface area, highly electrocatalytic activities and electronic conductivity. The researchers achieved the detection limit of 4.3×10 −13 M and confirmed that the new hybrid nanomaterial (MWNT/PPNWs/GNPs) and the DNA aptamer could be used to fabricate an electrochemical biosensor for virus detection [121]. Lee 10,000 pfu.mL -1 , with a detection limit of 50 pfu.mL -1 .…”

DNA probe modified with 3-iron bis(dicarbollide) for electrochemical determination of DNA sequence of Avian Influenza Virus H5N1

“…To our knowledge, all reported genosensors targeting the nucleotide sequence of H5N1 Avian Influenza Virus (AIV) were based on electrochemical detection employing either electrochemical impedance spectroscopy or cyclic voltammetry with exogenous redox markers present in supporting electrolyte (Kukol et al, 2008;Liu et al, 2011;Malecka et al 2013). …”

Electrochemical nanomaterial-based nucleic acid aptasensors

“…A sensitive electrochemical method for detection of avian influenza virus (AIV) H5N1 gene sequence using a DNA aptamer immobilized on to a CNT hybrid nanomaterial-modified electrode was developed in the work discussed in Ref. [103]. To enhance selectivity and sensitivity, the modified electrode was assembled with CNT, polypyrrole nanowires (PPNWs), and gold nanoparticles (GNPs).…”