Electrochemical detection of 8-hydroxy-2′-deoxyguanosine as a biomarker for oxidative DNA damage in HEK293 cells exposed to 3-chloro-1,2-propanediol

Abstract

Detection of 8-hydroxy-2′-deoxyguanosine generated via ROS-mediated oxidative DNA damage in HEK293 cells through a fabricated electrochemical sensor for evaluation of the cytotoxicity of 3-MCPD.

Long-term <i>in vitro</i> effects of exposing the human HK-2 proximal tubule cell line to 3-monochloropropane-1,2-diol

“…Taken together, the data presented in this study are in agreement with the short-term (up to 48 hour) exposure studies performed in vitro with other cell lines (Buhrke et al, 2018;Liu et al, 2012;Ozcagli et al, 2016;Senyildiz et al, 2017;Sun et al, 2015) and further support the notion that 3-MCPD may be a mild toxin towards human proximal tubule cells. Moreover, no adverse kidney (or other) events among humans have been attributed to 3-MCPD-associated food consumption or occupational exposure ("3-CHLORO-1,2-PROPANEDIOL (JEC-FA Food Additives Series 48)," 2001) and a thorough toxicological evaluation in a non-human primate model showed hematological problems instead of any renal effects (Kirton et al, 1970).…”

“…A plethora of in vivo studies done mostly in rat models have demonstrated the potential for 3-MCPD to induce a variety of renal and other effects in acute and chronic settings ("3-CHLORO-1,2-PROPANEDIOL (JECFA Food Additives Series 48)," 2001; Cho et al, 2008b;Huang et al, 2018;Onami et al, 2014). In vitro renal studies, however, have only been done in rat NRK-52E and human HEK or TK cell models to confirm the short-term effects of 24 or 48 hour exposures (Buhrke et al, 2018;Liu et al, 2012;Ozcagli et al, 2016;Senyildiz et al, 2017;Sun et al, 2015). Since the kidneys are among the list of organs targeted by 3-MCPD, we sought to investigate both the short-and long-term effects of directly exposing this compound to human proximal tubule cells in vitro.…”

“…They can also offer helpful insights into their mechanisms of toxicity over different periods of time. Despite these advantages, there is a lack of in vitro studies on the effects of 3-MCPD on proximal tubule cells beyond 48 hr (Buhrke et al, 2018;Liu et al, 2012;Ozcagli et al, 2016;Senyildiz et al, 2017;Sun et al, 2015). As such, we assessed the effects of treating HK-2 cells with varying concentrations of free 3-MCPD for up to 16 days to create an unprecedentedly stringent model of direct and continuous exposure.…”

Point of care diagnostics for carbonyl/oxidative stress biomarkers

“…subjects, respectively, thereby requiring sensitive methodologies for their assay [241,243]. The most commonly used analytical techniques for the detection of 8-OHdG in urine are high performance liquid chromatography-electrochemical detection (HPLC-ECD) [244], HPLC tandem mass spectrometry (HPLC MS/MS) [245], capillary electrophoresis with electrochemical detection (CE-ECD) [242,246], electrochemical methods including cyclic voltammetry (CV) [247][248][249], differential pulse voltammetry [250] and linear sweep voltammetry (LSV) [251]. Ultraviolet spectroscopy [252,253],…”

“…Electrochemical methodologies such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) for quantitative analysis of 8-OHdG have claimed subnanomolar detection sensitivities [247,250]. Similarly, spectroscopic methods such as ultraviolet-visible spectroscopy (UV-Vis) and fluorescence spectroscopy also have been explored for the detection of 8-OHdG [252][253][254] with subnanomolar and picomolar sensitivities.…”