Electrochemical Detection Coupled with High-Performance Liquid Chromatography in Pharmaceutical and Biomedical Analysis: A Mini Review

Wang, Chengyin; Xu, Jianyun; Zhou, Guiyou; Qu, Qishu; Yang, Gongjun; Hu, Xiaoyun

doi:10.2174/138620707782152362

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…The interest in the method resides also in the fact that the ECD can be easily miniaturised and used in fully integrated chip-based systems. HPLC-ECD is commonly used in many pharmaceutical, clinical, and biochemical laboratories [10], [44]. Because antioxidants are electrochemically active, HPLC-ECD is well suited for their accurate quantification.…”

Section: Hplc-ecd (Electrochemical Detection)mentioning

confidence: 99%

“…Some degree of correlation between the electrochemical profiles of 17 flavonoids and 3 cinnamic acid derivatives and 3 different antioxidant assays was established [52]. HPLC-ECD also has been used in many applications related to the analysis of drugs in body fluids [44], [10]. Artemisinin, for example, was selectively detected and quantified in plasma using a glassy carbon electrode by reductive ECD [52].…”

Section: Hplc-ecd (Electrochemical Detection)mentioning

confidence: 99%

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HPLC in Natural Product Analysis: The Detection Issue

2009

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High-performance liquid chromatography (HPLC) is a very powerful and versatile chromatographic technique for the separation of natural products (NPs) in complex matrices, such as crude extracts for selective detection and quantification or general profiling. The method is widespread and has been adapted to the analysis of a broad range of NPs generally without the need for complex sample preparation. The choice of the appropriate detection method in HPLC is crucial because of the diversity of NPs and the fact that there is no single technique for their efficient detection. In this review both qualitative and quantitative applications of HPLC with UV, DAD, FD, ECD, RID, FID, CL, ESLD, CAD, MS, MS-MS, and NMR are covered to provide a general, rather than an exhaustive, overview. The potential and limitations as well as some new trends in HPLC hyphenation are discusse

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Section: Hplc-ecd (Electrochemical Detection)mentioning

confidence: 99%

Section: Hplc-ecd (Electrochemical Detection)mentioning

confidence: 99%

HPLC in Natural Product Analysis: The Detection Issue

2009

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“…The authors would like to highlight that, after 15 years from a mini review related to electrochemical detection coupled with high-performance liquid chromatography in pharmaceutical and biomedical analysis, the problems related to the HPLC-ED coupling are almost the same (e.g., first among all, the ED performance in presence of the chromatographic gradient elution) [117]. The authors agree with the paper that coulometric array detectors are increasing their role, and are being used more due to their compatibility with HPLC system and their decreasing prices.…”

Section: Discussionmentioning

confidence: 99%

Review of the Analytical Methods Based on HPLC-Electrochemical Detection Coupling for the Evaluation of Organic Compounds of Nutritional and Environmental Interest

et al. 2022

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This review would like to show the state of the art regarding the coupling of High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection (ED). Since a universal detector for HPLC is not available, the electrochemical detection methods, thanks to their versatility and specificity, are competitive with respect to the detectors currently used. The papers present in literature on HPLC-ED technique are analyzed and discussed: for example, they regard the development of analytical determinations of resveratrol, rosmarinic acid, aromatic heterocyclic amines and glyphosate in food matrices such as meat, aromatic plants, vegetables, fruit and tomato juices. These papers show that electrochemical sensors used as detectors for HPLC can offer better sensitivity values than other detectors. Furthermore, the use of specific working potentials allows avoid matrix interferences to be avoided by almost exclusively determining the analytes of interest. It should be underlined that HPLC-ED methods have a selectivity that allows for limitation of the sample preparation and clean-up procedures to a minimum, making them quick and easy to apply. In addition, these methods offer advantages such as the possibility of direct analysis, that derivatization is often not necessary, the cost-effectiveness of the instrumentation and the possibility of regenerating the electrodes which allows numerous analyses in succession.

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“…More advanced applications of PED for carbohydrates have surfaced, including the "fingerprinting" of bioproducts such as tobacco and peptones, as well as the analysis of bacterial polysaccharides. In addition, in vitro microdialysis for carbohydrate systems has allowed for enzyme characterization and the monitoring of carbohydrate-based bioprocesses [42], significantly extending the list of applications in pharmaceutical and biomedical fields [72]. Carbohydrate/alditol-focused applications (38%) tend to show the greatest growth, especially for their determination in bioprocesses, followed by aminosugars/aminoglycosides (24%) and amines/amino acids (15%), see Table 1.…”

Section: Recent Applications Of Pedmentioning

confidence: 99%