Electrochemical deposition to construct a nature inspired multilayer chitosan/layered double hydroxides hybrid gel for stimuli responsive release of protein

Zhao, Pengkun; Liu, Youyu; Xiao, Ling; Deng, Hongbing; Du, Yumin; Shi, Xiaowen

doi:10.1039/c5tb01056j

Cited by 46 publications

(24 citation statements)

References 40 publications

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“…Numerous investigations have shown that coating of alginate microspheres with chitosan layer is and effective way of an active agent controlled release as a desirable characteristic for all delivery systems (Li et al, 2014;Lucinda-Silva, Salgado, & Evangelista, 2010;Wang & Zhao, 2013). Layering technology has been often utilized for producing novel materials with tailorable properties, such as, for example, multilayered chitosan/layered double hydroxides hybrid hydrogel as a promising controlled delivery system (Zhao et al, 2015(Zhao et al, , 2017.…”

Section: Introductionmentioning

confidence: 99%

Influence of surface morphology and structure of alginate microparticles on the bioactive agents release behavior

Jurić

Šegota

Vinceković

2019

Carbohydrate Polymers

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The structure-property relationship in alginate microparticles (microspheres and microcapsules prepared with or without Trichoderma viride spores (Tv) was investigated. Surface morphology, structure and release behavior from alginate microparticles strongly depend on calcium concentration and presence of Tv and chitosan layer. All microparticles exhibited a granular surface structure with substructures consisting of abundant smaller particles. In vitro active agents release study revealed that the increase in calcium cation concentration reduced the release rate of Tv (˜84% for microspheres;˜57% for microcapsules) and calcium cations (˜20% for microspheres;˜23% for microcapsules). The average decrease in k values due to chitosan layer addition is 41% for Tv and 93% for calcium ions, respectively. The underlying Tv release mechanism from microspheres is anomalous transport kinetics, whereas from microcapsules is controlled by Type II transport. The differences in microparticle surface properties did not affect the mechanism controlling calcium ions release detected as diffusion through microparticles.

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Section: Introductionmentioning

confidence: 99%

Influence of surface morphology and structure of alginate microparticles on the bioactive agents release behavior

Jurić

Šegota

Vinceković

2019

Carbohydrate Polymers

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“…It is therefore expected that a daidzein-loaded microsphere coating system will possess the potential to stimulate bone related cells for enhanced osseointegration. Taking advantage of the controllable drug release performance from PHBV microspheres 18 and the simplicity of the electrophoretic deposition (EPD) technique to produce polymer coatings on metallic substrates, 19,20 PHBV microspheres were able to be stabilized into a polymer coating, and a minimized and prolonged drug release rate could be expected. Combination of PHBV microspheres and biopolymers was investigated by EPD at first.…”

Section: Introductionmentioning

confidence: 99%

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Chen

Yao

et al. 2016

J. Mater. Chem. B

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The development of drug delivery coating systems for local and long-term drug release is gaining increasing interest especially to functionalize bioinert implants with osseointegration and antibacterial properties. In this study, a biodegradable drug delivery coating platform consisting of drug-loaded PHBV microspheres embedded in an alginate-PVA matrix was fabricated by a one-step electrophoretic deposition (EPD) process. Layer by layer (LbL) deposition was exploited to generate chitosan-alginate multilayers on the EPD-produced coating to enlarge the diffusional barrier around the microspheres for controlled drug release. Daidzein, selected as a model drug due to its anti-osteoporosis properties, was pre-encapsulated in PHBV microspheres. The parameters for microsphere fabrication were optimized by an orthogonal design approach. The loading efficiency of daidzein in both the microspheres and in the deposited coatings was adjusted by varying the processing parameters during microsphere fabrication and the EPD process. The degradation of the deposited multilayers was investigated in PBS for up to 14 days. The degradation rate, surface roughness and wettability, as well as adhesion strength of the coatings during degradation were evaluated by applying a range of techniques. A controlled and sustained daidzein release was detected from both free microspheres and microsphere-containing coatings. Finally cytotoxicity and stimulatory effects of daidzein or daidzein-loaded coatings, on both MC3T3-E1 and RAW264.7 cell lines, were studied to validate the potential of the developed coatings for orthopedic applications.

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“…Firstly, insulin was loaded on LDH according to the method of Zhao et al [23]. A chitosan solution that comprised 8 mg of chitosan, 4 mL of an aqueous solution of acetic acid (1% v/v) and NaOH at pH 6.5 was prepared.…”

Section: Preparation Of Novel Ldh-ins-csnpsmentioning

confidence: 99%

Characterization and Efficacy of A Novel Formula of Insulin Chitosan Layered Duple Hydroxide-Nanohybrid for Oral Administration

El-Shahawy¹,

Abdel-Moneim²,

Eldin³

et al. 2020

Nano BioMed ENG

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The development of an oral insulin therapy remains an ultimate goal to both enhance ease of use, and to provide therapeutic advantages rooted in its direct delivery to the portal vein and liver. The current study aimed to develop a novel formula for insulin oral administration against experimental diabetes in rats. Entrapped insulin (INS) between chitosan nanoparticles (CSNPs) and layered duple hydroxide (LDH) (LDH-INS-CSNPs) was chemically prepared, and characterized by X-ray diffraction, Fourier transformation infrared, high-resolution transmission electron microscope, field emission scanning electron microscope and Zeta potential measurements. The insulin release study was conducted in vitro, while the oral hypoglycemic effect of LDH-INS-CSNPs was investigated in vivo in diabetic rats. The prepared formulas revealed a variation in the spectra of characterization methods. The insulin entrapment between LDH and chitosan avoided the burst release of insulin and acid denaturation in the stomach and enzymatic degradation throughout the gut. Consequently, the blood glucose level of LDH-INS-CSNPs formula exhibited a marked hypoglycemic effect. The present work showed that the LDH-INS-CSNPs formula had a protective effect against enzymatic degradation, reduced insulin initial burst release, and enhanced bioavailability through the oral administration route. Interestingly, the presented formula could be an oral antidiabetic agent alternative to injectable insulin.

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Electrochemical deposition to construct a nature inspired multilayer chitosan/layered double hydroxides hybrid gel for stimuli responsive release of protein

Cited by 46 publications

References 40 publications

Influence of surface morphology and structure of alginate microparticles on the bioactive agents release behavior

Influence of surface morphology and structure of alginate microparticles on the bioactive agents release behavior

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Characterization and Efficacy of A Novel Formula of Insulin Chitosan Layered Duple Hydroxide-Nanohybrid for Oral Administration

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